阿克苏、连云港和淮安城市居民头发中氢、氧稳定同位素比值差异研究

人类头发中氢、氧稳定同位素比值（δ²H、δ¹⁸O）与其生活的地理位置具有相关性。为考察不同城市常住居民头发中δ²H、δ¹⁸O的差异性，本研究选择了西部的阿克苏和东部沿海的连云港、淮安3个城市常住居民的头发样本为研究对象，利用元素分析仪-稳定同位素比质谱仪（EA-IRMS）进行氢、氧稳定同位素比值检验，采用Bayes算法对检验结果进行判别分析。同时，采用波长扫描光腔衰荡光谱仪对3个城市的居民饮用水进行氢、氧稳定同位素比值检验，分析居民头发与其饮用水中氢、氧稳定同位素比值的相关性。结果表明：阿克苏与连云港、淮安的常住居民头发中氢、氧稳定同位素比值差异显著（P<0.05）；连云港与淮安的差异不显著（P>0.05）。Bayes判别分析结果表明：阿克苏与连云港、淮安的检测结果能够实现较好的区分，而连云港与淮安的区分效果较差；利用δ²H和δ¹⁸O共同作为判别因子的判别准确率高于单独使用δ¹⁸O或δ²H。3个城市常住居民头发与其饮用水中氢、氧稳定同位素比值具有相关性（δ²H：r=0.933；δ¹⁸O：r=0.998）。

Study on the Difference of Hydrogen and Oxygen Stable Isotope Ratios of Aksu,Lianyungang and Huai’an Urban Residents’ Hair

Stable isotope analysis has become one of the main trends in sourcing research. The isotopic ratios of carbon, hydrogen, oxygen, nitrogen, sulfur and other elements measured by isotope ratio mass spectrometry (IRMS) have been widely applied in geology, hydrology, agriculture, medical and environmental science. The stable isotope ratios of hydrogen and oxygen in atmospheric water vary significantly along geographical gradient. The differences in the stable isotope ratios of hydrogen and oxygen in water at different geographical locations can be reflected in human tissues, such as hair, nail, teeth, skeleton and so on, through biological metabolism. So the stable isotope ratios of hydrogen and oxygen in human hair (δ²H,δ¹⁸O) are related to the geographical location of their living cities. In this paper, the differences ofδ²H andδ¹⁸O of urban residents’ hair living in 3 different cities, Aksu in the west and Lianyungang and Huai’an on the eastern coast in China were investigated. 79 hair samples of the residents living in the same city for the past 8 months were collected from the three cities, and 41 samples of their drinking water were also collected. The stable isotope ratios of hydrogen and oxygen of hair samples were determined by elemental analyzer-stable isotope ratio mass spectrometer (EA-IRMS). The data was analyzed by the Bayesian algorithm. Meanwhile, the hydrogen and oxygen stable isotope ratios of the drinking water in the three cities were determined by wavelength-scanning cavity ring-down spectrometer. The correlation of the stable isotope ratio of hydrogen and oxygen between the hair and drinking water was analyzed. The results showed that the stable isotope ratios of hydrogen and oxygen of Aksu, another two cities urban residents’ hair are significantly different (P<0.05), while there is no significant difference between Lianyungang and Huai’an urban residents’ hair (P<0.05). The results of Bayes discriminant analysis also showed thatδ²H andδ¹⁸O of hair between Aksu and Lianyungang, Huai’an residents can be better classified, while another two cities are less effective. The discriminative accuracy of usingδ²H andδ¹⁸O as discriminant factors is higher than that of usingδ¹⁸O orδ²H separately. The stable isotope ratio of hydrogen and oxygen of hair were correlated with their drinking water (δ²H: r=0.933;δ¹⁸O: r=0.998).