聚氨酯/胶原蛋白复合纳米纤维支架的性能

针对聚氨酯（PU）力学性能较好，亲水性较差，细胞在其表面的黏附较少，不适宜单独作为血管组织工程支架材料的问题，为提高PU 纳米纤维支架的亲水性和生物相容性，通过静电纺丝制备不同比例的复合聚氨酯／胶原蛋白纳米纤维支架，利用扫描电镜、红外光谱仪等观察测试纳米纤维支架材料的形态结构、化学性能等；并在纳米纤维支架表面培养血管平滑肌细胞，通过扫描电镜观察细胞在支架表面的生长情况。结果表明：胶原蛋白的加入使纤维直径从453nm减小到了154nm，平均孔径尺寸从0.64 μｍ 降低到了0.28 μｍ，而且增加了细胞在其表面的黏附和增殖；当聚氨酯和胶原蛋白的质量比为3:1时，纳米纤维支架的拉伸强度最大，生物相容性最好，表面黏附生长的细胞最多。

Performance of composite polyurethane/collagen nanofiber scaffolds

Polyurethanehas excellent mechanical property, however, pure PU is not suitable as vascular tissue engineering scaffold due to its bydrophobicity andless cells were attached on its surface. In this paper, composite polyurethane/collagennanofibrous scaffolds with different ratio for vascular tissue engineering were prepared through electrospinning. Firstly, the morphologies, chemical and mechanical characterizations were carried out using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) respectively. Then cultured smooth muscle cells (SMCs) on nanofibrous scaffolds and evaluated the attachment and proliferation of cells using SEM. The results show that the addition of collagen decreased the diameter of nanofibers from 453 nm to 154 nm, decreased the pore diameter from 0.64μm to 0.28μm, also enhance the proliferation and attachment of cells. When the mass ratio of polyurethane and collagen is3:1, the tensile strength and biocompatibility of composite PU/Coll(3:1) scaffolds were the best, hence, more SMCs were observed on this scaffold.