| 压电体声波生物传感器检测食品中的结核杆菌 |
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| 引用本文： | 袁河清,李萍,刘有胜,周长胜,曹明.压电体声波生物传感器检测食品中的结核杆菌[J].食品科学,2009,30(2):201-203. |
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| 作者姓名： | 袁河清 李萍 刘有胜 周长胜 曹明 |
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| 作者单位： | 1. 长沙环境保护职业技术学院
2. 湖南大学环境科学与工程学院 |
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| 摘 要： | 本实验使用一个压电体声波生物传感器对模拟样品中的结核杆菌减毒菌株H37Ra 进行快速检测，在池系数为0.513cm 和培养基稀释倍数为10 倍时，传感器具有较高的灵敏度，并得到了细胞浓度与频率之间的线性方程；使用2% NaOH 处理样品，能去除杂菌的干扰，但会使检测时间延长。
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| 关 键 词： | 结核杆菌 压电体声波生物传感器 食品检测 |
| 收稿时间： | 2007-11-22 |
Detection of Mycobacterium tuberculos in Food Using Piezoelectric Bulk Acoustic Wave Biosensor |
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| YUAN He-qing,LI Ping,LIU You-sheng,ZHOU Chang-sheng,CAO Ming.Detection of Mycobacterium tuberculos in Food Using Piezoelectric Bulk Acoustic Wave Biosensor[J].Food Science,2009,30(2):201-203. |
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| Authors: | YUAN He-qing LI Ping LIU You-sheng ZHOU Chang-sheng CAO Ming |
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| Affiliation: | (1. Changsha Environmental Protection College, Changsha 410004, China；
2. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China) |
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| Abstract: | Mycobacterium tuberculosis is the pathogenic microorganism of tuberculosis, and it could be infected by contaminated food. A piezoelectric bulk acoustic wave biosensor was used for rapid detection of M. tuberculos attenuated strain H37Ra in food samples. With the pool coefficient of 0.513 cm and the 10-fold diluted culture medium, it was found that the biosensor had a relatively high detection sensitivity, and a linear equation between cell concentrations and wave frequency was also constructed. It was also found that a pre-treatment with 2% NaOH solution could avoid the interference of mixed bacteria but prolong the detection period. |
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| Keywords: | Mycobacterium tuberculos piezoelectric bulk acoustic wave biosensor food detection |
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