Glypican 3 and glypican 4 are juxtaposed in Xq26.1 |
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Authors: | R Huber R Mazzarella CN Chen E Chen M Ireland S Lindsay G Pilia L Crisponi |
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Affiliation: | Department of Gastrointestinal and Liver Diseases, Academic Medical Center, The Netherlands. |
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Abstract: | This paper reports the development of a dual column system for the simultaneous separation of fluorescent short-chain ceramide, 6-[(7-nitrobenz-2-oxa-1,3,-diazol-4-yl[NBD])amino]hexanoyl-sphingo sine and its metabolites, C6-NBD-sphingomyelin and C6-NBD-glucosylceramide, as well as the fluorescent derivatives of choline and serine phosphatides. The method enables the separation of these lipids in a single run on the basis of the polarity of their headgroups and hydrophobicity of their acyl backbone. The fluorescent properties of the NBD-label make it possible to quantitate small amounts of NBD-lipid analogues. The sensitivity of the presented method thus permits the use of small sample volumes and the determination of NBD-lipid analogues secreted into mouse bile directly, without prior extraction or concentration steps. |
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