Improved resolution of31P nuclear magnetic resonance spectra of phospholipids from brain |
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| Authors: | G. Capuani T. Aureli A. Miccheli M. E. Di Cocco M. T. Ramacci M. Delfini |
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| Affiliation: | (1) Institute for Research on Senescence, Sigma Tau, 00040 Pomezia (Rome), Italy;(2) Department of Chemistry, University of Rome “La Sapienza”, Rome, Italy |
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| Abstract: | A method is described wherein the resolution of31P nuclear magnetic resonance spectra of the lipophilic fraction from a Bligh-Dyer extract of rat brain can be enhanced. The lipids are dispersed as micelles in aqueous solution with sodium deoxycholate, and spectral resolution is further optimized by adjusting the pH and by removing ions from the solution. The application of the method to the study of aging in rat brain serves as an example. |
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