Irreversible Protein Labeling by Paal–Knorr Conjugation |
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| Authors: | Ramesh Dasari Dr James J La?Clair Prof Alexander Kornienko |
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| Affiliation: | 1. Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, USA;2. Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, USA;3. Xenobe Research Institute, San Diego, CA, USA |
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| Abstract: | The application of new chemical reactions in a biological context has advanced bioconjugation methods for both fundamental research and commercial arenas. Recent adaptations of reactions such as Huisgen 1,3‐dipolar or Diels–Alder cycloadditions have enabled the labeling of specific residues in biomolecules by the attachment of molecules carrying azides, alkynes, or strained alkenes. Although these are fundamental tools, there is a need for the discovery of reactions that can label native proteins. We report herein the adaptation of the Paal–Knorr reaction to label lysine residues in proteins via pyrrole linkages. |
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| Keywords: | biocompatible reactions bioconjugation click chemistry Paal– Knorr reaction protein labeling |
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