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Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide‐Modified Quantitative PCR |
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| Authors: | Dr Yu Luo Hannah L Bolt Dr Gabriela A Eggimann Prof?Dr Danny F McAuley Dr Ronan McMullan Dr Tanya Curran Dr Mei Zhou Professor Colin A B Jahoda Dr Steven L Cobb Dr Fionnuala T Lundy |
| Affiliation: | 1. Centre for Experimental Medicine, The Wellcome–Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK;2. Durham University, Department of Chemistry, Biophysical Sciences Institute, Durham, UK;3. Regional Virus Laboratory, Kelvin Building, Royal Victoria Hospital, Belfast Health and Social Care Trust, Belfast, UK;4. School of Pharmacy, Queen's University Belfast, Belfast, UK;5. Durham University, School for Biological and Biomedical Sciences, Durham, UK |
| Abstract: | Biofilms containing Candida albicans are responsible for a wide variety of clinical infections. The protective effects of the biofilm matrix, the low metabolic activity of microorganisms within a biofilm and their high mutation rate, significantly enhance the resistance of biofilms to conventional antimicrobial treatments. Peptoids are peptide‐mimics that share many features of host defence antimicrobial peptides but have increased resistance to proteases and therefore have better stability in vivo. The activity of a library of peptoids was tested against monospecies and polymicrobial bacterial/fungal biofilms. Selected peptoids showed significant bactericidal and fungicidal activity against the polymicrobial biofilms. This coupled with low cytotoxicity suggests that peptoids could offer a new option for the treatment of clinically relevant polymicrobial infections. |
| Keywords: | antibacterial antibiotics antiproliferation cross kingdom peptoid quantification |