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Copper is a Cofactor of the Formylglycine‐Generating Enzyme |
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| Authors: | Matthias Knop Thanh Quy Dang Prof?Dr Gunnar Jeschke Prof?Dr Florian P Seebeck |
| Affiliation: | 1. Department of Chemistry, University of Basel, Basel, Switzerland;2. Laboratory of Physical Chemistry, ETH Zürich, Zürich, Switzerland |
| Abstract: | Formylglycine‐generating enzyme (FGE) is an O2‐utilizing oxidase that converts specific cysteine residues of client proteins to formylglycine. We show that CuI is an integral cofactor of this enzyme and binds with high affinity (KD=of 10?17 m ) to a pair of active‐site cysteines. These findings establish FGE as a novel type of copper enzyme. |
| Keywords: | copper EPR formylglycine oxidases oxygen activation protein engineering |