Mutation of Conserved Residues Increases in Vitro Activity of the Formylglycine‐Generating Enzyme |
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Authors: | Matthias Knop Roxana Lemnaru Prof. Dr. Florian P. Seebeck |
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Affiliation: | Department of Chemistry, University of Basel, Basel, Switzerland |
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Abstract: | The formylglycine‐generating enzyme (FGE) recognizes proteins with a specific cysteine‐containing six‐amino‐acid motif and converts this cysteine residue into formylglycine. The resulting aldehyde function provides a unique handle for selective protein labeling. We have identified two mutations in FGE from Thermomonospora curvata that increase this catalytic efficiency more than 40‐fold. The resulting activity and stability, as well as its ease of recombinant production, make this FGE variant a practical reagent for in vitro protein engineering. |
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Keywords: | copper formylglycine mutagenesis posttranslational modification protein engineering |
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