Synthetic Biomarker Design by Using Analyte‐Responsive Acetaminophen |
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Authors: | Dr. Tatsuya Nishihara Dr. Joe Inoue Dr. Sho Tabata Dr. Shinnosuke Murakami Dr. Takamasa Ishikawa Dr. Natsumi Saito Dr. Shinji Fukuda Prof. Dr. Masaru Tomita Prof. Dr. Tomoyoshi Soga |
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Affiliation: | 1. Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan;2. National Institute of Technology, Tsuruoka College, Tsuruoka, Yamagata, Japan |
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Abstract: | The use of synthetic biomarkers is an emerging technique to improve disease diagnosis. Here, we report a novel design strategy that uses analyte‐responsive acetaminophen (APAP) to expand the catalogue of analytes available for synthetic biomarker development. As proof‐of‐concept, we designed hydrogen peroxide (H2O2)‐responsive APAP (HR‐APAP) and succeeded in H2O2 detection with cellular and animal experiments. In fact, for blood samples following HR‐APAP injection, we demonstrated that the plasma concentration ratio [APAP+APAP conjugates]/[HR‐APAP] accurately reflects in vivo differences in H2O2 levels. We anticipate that our practical methodology will be broadly useful for the preparation of various synthetic biomarkers. |
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Keywords: | acetaminophen hydrogen peroxide liquid chromatography mass spectrometry synthetic biomarker |
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