Direct Intracellular Delivery of Cell‐Impermeable Probes of Protein Glycosylation by Using Nanostraws |
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| Authors: | Dr Alexander M Xu Derek S Wang Dr Peyton Shieh Yuhong Cao Prof Nicholas A Melosh |
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| Affiliation: | 1. Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA;2. Present address: Chemistry and Chemical Engineering Division, California Institute of Technology, Pasadena, CA, USA;3. Department of Chemistry, Stanford University, Stanford, CA, USA |
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| Abstract: | Bioorthogonal chemistry is an effective tool for elucidating metabolic pathways and measuring cellular activity, yet its use is currently limited by the difficulty of getting probes past the cell membrane and into the cytoplasm, especially if more complex probes are desired. Here we present a simple and minimally perturbative technique to deliver functional probes of glycosylation into cells by using a nanostructured “nanostraw” delivery system. Nanostraws provide direct intracellular access to cells through fluid conduits that remain small enough to minimize cell perturbation. First, we demonstrate that our platform can deliver an unmodified azidosugar, N‐azidoacetylmannosamine, into cells with similar effectiveness to a chemical modification strategy (peracetylation). We then show that the nanostraw platform enables direct delivery of an azidosugar modified with a charged uridine diphosphate group (UDP) that prevents intracellular penetration, thereby bypassing multiple enzymatic processing steps. By effectively removing the requirement for cell permeability from the probe, the nanostraws expand the toolbox of bioorthogonal probes that can be used to study biological processes on a single, easy‐to‐use platform. |
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| Keywords: | click chemistry drug delivery fluorescent probes glycosylation nanotubes |
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