An Efficient Glycoblotting‐Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein |
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Authors: | Dr. Takayuki Furukawa Dr. Hiroshi Hinou Dr. Seiji Takeda Prof. Hitoshi Chiba Prof. Shin‐Ichiro Nishimura Prof. Shu‐Ping Hui |
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Affiliation: | 1. Graduate School of Health Science, Hokkaido University, Kita-ku, Sapporo, Japan;2. Graduate School of Life Science, Hokkaido University, Kita-ku, Sapporo, Japan |
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Abstract: | Although widely occurring lipid oxidation, which is triggered by reactive oxygen species (ROS), produces a variety of oxidized lipids, practical methods to efficiently analyze oxidized lipids remain elusive. Herein, it is shown that the glycoblotting platform can be used to analyze oxidized lipids. Analysis is based on the principle that lipid aldehydes, one of the oxidized lipid species, can be captured selectively, enriched, and detected. Moreover, 3‐methyl‐1‐p‐tolyltriazene (MTT) methylates phosphoric and carboxylic acids, and this MTT‐mediated methylation is, in combination with conventional tandem mass spectrometry (MS/MS) analysis, an effective method for the structural analysis of oxidized lipids. By using three classes of standards, liposomes, and a lipoprotein, it is demonstrated that glycoblotting represents a powerful approach for focused lipidomics, even in complex macromolecules. |
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Keywords: | analytical methods glycoblotting lipids lipoproteins oxidation |
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