The 5′‐AG5CC‐3′ Fragment from the Human CPEB3 Ribozyme Forms an Ultrastable Parallel RNA G‐Quadruplex |
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| Authors: | Katharina Kulikov Dr. Senada Nozinovic Dr. Stephanie Kath‐Schorr |
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| Affiliation: | 1. LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Bonn, Germany;2. Institute for Inorganic Chemistry, University of Bonn, Bonn, Germany |
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| Abstract: | An unusually thermostable G‐quadruplex is formed by a sequence fragment of a naturally occurring ribozyme, the human CPEB3 ribozyme. Strong evidence is provided for the formation of a uniquely stable intermolecular G‐quadruplex structure consisting of five tetrad layers, by using CD spectroscopy, UV melting curves, 2D NMR spectroscopy, and gel shift analysis. The cationic porphyrin TMPyP4 destabilizes the complex. |
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| Keywords: | circular dichroism CPEB3 ribozyme G-quadruplexes RNA RNA structures |
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