| 钟韵梅,丛梅梅,牟善婷,王若菲,马 杰.QuEChERS-气相色谱-质谱联用法快速检测植物源性食品中24种农药残留[J].食品安全质量检测学报,2019,10(10):3132-3137 |
| QuEChERS-气相色谱-质谱联用法快速检测植物源性食品中24种农药残留 |
| Determination of 24 kinds of pesticide residues in plant-derived foods by QuEChERS-gas chromatography-mass spectrometry |
| 投稿时间：2019-01-03 修订日期：2019-05-06 |
| DOI： |
| 中文关键词: QuEChERS 气相色谱-质谱联用法 农药残留 植物源性食品 |
| 英文关键词:QuEChERS gas chromatography-mass spectrometry pesticide residue plant-derived foods |
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| 摘要点击次数: 834 |
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| 中文摘要: |
| 目的 建立QuEChERS-气相色谱-质谱联用法(gas chromatography-mass spectrometry, GC/MS) 测定植物源性食品中有机磷、有机氯、拟除虫菊酯类等24种农药残留的检测技术。方法 植物源性食品中多组分农药残留通过乙腈与QuEChERS试剂包提取、净化、浓缩, 经气相色谱质谱联用仪分析测定, 选择离子模式下监测, 外标法定量。结果 在0.010~1.0 mg/L范围内, 24种农药的质量浓度与对应的峰面积间呈良好的线性关系, 相对系数均大于0.994。分别取蘑菇、山药、苹果为空白基质样品, 以0.1 mg/kg浓度进行加标实验, 24种农药的平均回收率在71.4%~108.7%之间, 相对标准偏差(relative standard deviation, RSD)在2.03%~11.78%(n=6)之间。方法的检出限2~5 μg/kg, 定量限为6~15 μg/kg。结论 该方法简单、快速、准确、灵敏度高, 可用于植物性样品中多种农药残留的同时检测分析。 |
| 英文摘要: |
| Objective To establish a method for determination of 24 pesticide residues such as organophosphorus, organochlorine and pyrethroids in plant-derived foods by QuEChERS-gas chromatography-mass spectrometry (GC/MS). Methods Multi-component pesticide residues in plant-derived foods were extracted, purified and concentrated by acetonitrile and QuEChERS reagents. The samples were analyzed by gas chromatography-mass spectrometry, monitored under selected ion mode and quantified by external standard method. Results In the range of 0.010~1.0 mg/L, the mass concentration of 24 pesticides showed a good linear relationship with the corresponding peak area, and the relative coefficients were all greater than 0.994. Samples of mushroom, yam and apple were taken as blank substrate respectively, and the standard experiment was carried out at the concentration of 0.1 mg/kg. The average recovery of 24 pesticides ranged from 71.4% to 108.7%, and the relative standard deviations were between 2.03% and 11.78% (n=6). The limit of detection of method were 2-5 μg/kg and the limit of quantitation were 6-15 μg/kg. Conclusion This method is simple, rapid, accurate and sensitive, which can be used for simultaneous detection and analysis of various pesticide residues in plant samples. |
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