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为区分动脉粥样硬化斑块和血管壁,给激光血管成形术的实时监控提供依据,研究了动脉粥样硬化斑块和血管壁的激光诱导荧光光谱(LIFS)和拉曼光谱.日本雄性大耳白兔15只,以高脂饲料喂养3个月,取主动脉弓作纵行切开,分别采用380 nm紫外激光和532 nm绿激光作为激发光源诱导动脉粥样硬化斑块和血管肇的荧光光谱;采用532 nm绿激光作为激发光源诱导动脉粥样硬化斑块和血管壁的拉曼光谱.结果表明,380 nm紫外激光诱导的动脉粥样硬化斑块和血管壁的荧光光谱在416 nm处均有明显特征峰,但前者的强度显著高于后者;532 nm绿激光诱导的动脉粥样硬化斑块和血管壁的荧光光谱在800 nm处均有明显特征峰,但强度无明显差别;动脉粥样硬化斑块的拉曼光谱在3000 nm和3300 nm处存在明显的波峰和波谷,而血管壁的拉曼光谱曲线较光滑,无明显特征峰.说明380 nm紫外激光诱导的荧光光谱和拉曼光谱均有可能有效区分动脉粥样硬化斑块和血管壁,而532 nm绿激光诱导的荧光光谱不能有效区分动脉粥样硬化斑块和血管壁. 相似文献
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Turner spectroscopic system which achieved a spectral resolution of less than 1 nm in the range from 380 nm to 780 nm. Then, the calibration light source’s spectrum matching model is established and the digital micromirror device (DMD)’s surface illuminance distribution law is simulated and analyzed. Finally, the MOR error of the calibrated light source is inverted. The results show that the simulation spectrum error of the 2 700 K absolute color temperature is below ±7.4% in the wavelength range from 380 nm to 780 nm, and the MOR error meets the requirements of the International Civil Aviation Organization in 40—2 000 m of MOR. 相似文献
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在18至300 K的温度范围内,对聚乙炔的一种单取代衍生物的荧光特性进行了研究.在室温时,这种聚乙炔的单取代衍生物的薄膜能够发出强的绿色荧光,其荧光光谱的主要荧光峰位于510 nm,而它的两个次要荧光峰分别位于440 nm和380 nm.位于510、440 nm的两个荧光峰分别是该高分子材料所形成的激发缔合物的主要和次要发光峰,而位于380 nm的荧光峰是单条高分子链的发光峰.当温度从300 K降到18 K的过程中,原荧光光谱发生中的激发缔合物的主要发光峰从510 nm逐渐红移到570 nm,而其激发缔合物的次要发光峰逐渐消失;与此同时,该高分子材料的380 nm的荧光峰逐渐与主荧光峰分开.这些光谱方面的变化可用该高分子在低温下所发生的结构上的变化来解释. 相似文献
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<正> LP2983是一种微功耗、输出低电压(≤1.2V)线性稳压器(输出固定电压有0.9、1.0V及1.2V三种),输出电流可达150mA。该器件主要特点:输出电压精度可达±1%;能接受高的峰值电流(峰值输出电流典型值250mA,短路电流典型值400mA);低功耗(在150mA输出时典型地电流I_(GND)=825μA;空载时I_(GND)=65μA);输入电压范围宽(2.2~16V);有过热、过流保护电路;工作结温范围-40~+125℃; 小尺寸SOT-23封装;有关闭控制,关闭状态时,耗电<2μA。 LP2983有A级及标准级两种输出精度标准(A级的初始精度为±1%,标准级初始精度为±1.5%)。A级精度用后缀AIM5,标准级用后缀IM5。另外,三种不同输出电压分别用后缀-0.9、-1.0及-1.2表示0.9、1.0V及1.2V。 相似文献
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分别用声光晶体对氩离子激光515nm谱线作幅度和频率调制,实验研究了碘吸收的差拍饱和吸收信号。用频率调制方法得到斜率极陡的一阶微商饱和吸收信号,并将它作为鉴频曲线把氩离子激光频率稳到~(127)I_2分子的P(13)43-0的a_3线上。通过与一稳定到相同精细分量上的稳频氩离子激光器拍频,绐出激光频率稳定性优于±4.7×10~(-12)。 相似文献
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具有几十余种化学成分的丹参粉是典型的多组分物质,应用传统二维荧光光谱难以对其作定量分析。为了研究多组分共存的丹参粉水溶液的荧光特征,利用三维同步荧光光谱法结合平行因子分析法(PARAFAC)、分半信度法(split half method)、残差平方和(sum of squared error),解析光谱重叠严重的丹参粉水溶液荧光光谱。经上述方法分析确定丹参粉水溶液的平行因子模型组分数为4,其相应组分荧光特征为:第1组分荧光特征λEx=470~480 nm,λEm=520~530 nm;第2组分荧光特征λEx=400~410 nm,λEm=490~500 nm;第3组分荧光特征λEx=370~380 nm,λEm=460~470 nm;第4组分荧光特征λEx=560~570 nm,λEm=620~630 nm。其中第2组分的荧光特征可能是丹酚酸B的荧光特征。实验结果表明,在多组分共存且有较多干扰物质时,三维同步荧光结合平行因子算法可以简化光谱分析,较好地表征丹参的整体荧光特性。本研究为多组分共存的中药材分析提供了建议与帮助。 相似文献
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Bachtel AD Gray RA Stohlman JM Bourgeois EB Pollard AE Rogers JM 《IEEE transactions on bio-medical engineering》2011,58(7):2120-2126
We developed a new method for ratiometric optical mapping of transmembrane potential (V(m)) in cardiac preparations stained with di-4-ANEPPS. V(m)-dependent shifts of excitation and emission spectra establish two excitation bands (<481 and >481 nm) that produce fluorescence changes of opposite polarity within a single emission band (575-620 nm). The ratio of these positive and negative fluorescence signals (excitation ratiometry) increases V(m) sensitivity and removes artifacts common to both signals. We pulsed blue (450 ± 10 nm) and cyan (505 ± 15 nm) light emitting diodes (LEDs) at 375 Hz in alternating phase synchronized to a camera (750 frames-per-second). Fluorescence was bandpass filtered (585 ± 20 nm). This produced signals with upright (blue) and inverted (cyan) action potentials (APs) interleaved in sequential frames. In four whole swine hearts with motion chemically arrested, fractional fluorescence for blue, cyan, and ratio signals was 1.2 ± 0.3%, 1.2 ± 0.3%, and 2.4 ± 0.6%, respectively. Signal-to-noise ratios were 4.3 ± 1.4, 4.0 ± 1.2, and 5.8 ± 1.9, respectively. After washing out the electromechanical uncoupling agent, we characterized motion artifact by cross-correlating blue, cyan, and ratio signals with a signal with normal AP morphology. Ratiometry improved cross-correlation coefficients from 0.50 ± 0.48 to 0.81 ± 0.25, but did not cancel all motion artifacts. These findings demonstrate the feasibility of pulsed LED excitation ratiometry in myocardium. 相似文献
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Collaert N. Dixit A. Goodwin M. Anil K.G. Rooyackers R. Degroote B. Leunissen L.H.A. Veloso A. Jonckheere R. De Meyer K. Jurczak M. Biesemans S. 《Electron Device Letters, IEEE》2004,25(8):568-570
In this letter, we have fabricated a functional FinFET ring oscillator with a physical gate length of 25 nm and a fin width of 10 nm, the smallest ever reported. We demonstrate that these narrow (W/sub fin/ = 10 nm) and tall (H/sub fin/ = 60 - 80 nm) fins can be reliably etched with controlled profiles and that they are required to keep the short-channel effects under control, resulting in drain-induced barrier leakage characteristics of 45 mV/V at V/sub dd/ = 1 V and L/sub g/ = 25 nm for the nFET. For these ultrathin (10 nm) fins, we have succeeded in properly setting the V/sub T/ at 0.2 V without the use of metal gates. In addition to ring oscillators, we also have obtained excellent pFET FinFET devices at wider fin widths (W/sub fin/ = 65 nm) with I/sub dsat/ = 380 /spl mu/A//spl mu/m at I/sub off/ = 60 nA//spl mu/m and V/sub dd/ = -1.2 V. 相似文献
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Charge recombination in reaction center (RC) of photosystem II(PS II) is regarded as the location of 685 nm delayed fluorescence
(DF). The mechanism of 730 nm component appearing in the DF spectrum for chloroplast was studied by various spectral analysis
methods. Experimental results of the DF spectrum at different chloroplast concentration show that the intensity of peaks at
685nm and 730 nm ascends with the chloroplast concentration increasing when the concentration is relatively low. When the
concentration increases to the level of 7.8μg/ml, a maximum intensity of the peak at 685 nm appears but the intensity of 730
nm peak still increases. The peak at 730 nm finally reaches a maximum intensity at the chloroplast concentration of 31.2 μg/ml
while the intensity of the 685 nm peak has apparently fallen down. The results of absorption spectrum show that the ratios
of A685 to A730 keep almost constant with the increasing of chloroplast concentration. Furthermore, the excitation spectrum
for 730 nm fluorescence shows that the 685nm light has high excitation efficiency. These results indicate that the 730 nm
component of DF spectrum is the fluorescence of chlorophyll in PS I RC excited by 685 nm DF. Meanwhile, this can be further
verified by the invariability of DF spectrum at different delay time (1 second∼9 seconds).
This research is supported by the National Natural Science Foundation of China (60378043), and supported by the Opening Foundation
of the Key Laboratory of Opto-Electronic Technology and Intelligent Control (Lanzhou Jiaotong University), Ministry of Education
(K04108) 相似文献
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选用天然黑色海水珍珠、活性超级黑染色黑珍珠、辐照处理黑珍珠和硝酸盐染色黑珍珠,对其进行拉曼光谱测试、紫外可见吸收光谱测试的研究。两项测试表明:拉曼光谱中天然黑色海水珍珠、硝酸银染色和辐照黑珍珠均出现1080cm-1和699cm-1文石的特征拉曼峰,辐照黑珍珠荧光背景较强,染料染色黑珍珠的出峰位置与所使用染料的类型有关;紫外可见吸收光谱中,天然黑色海珍珠出现284nm和357nm两个吸收峰,染料活性超级黑染色珍珠出现380nm吸收峰,应为357nm吸收峰发生红移所致,银盐染色黑珍珠和辐照处理黑珍珠紫外区吸收峰消失。根据拉曼和紫外可见光谱特征,可以快速、无损地鉴定天然黑色珍珠和各种着色黑珍珠。 相似文献
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激光诱发荧光用于结肠肿瘤早期诊断 总被引:3,自引:0,他引:3
激光诱导荧光法(LaserInduced Fluorescence LIF)利用生物组织的自体荧光特性判断组织性质,能实时、无损地提供组织信息,从而区分正常与病变组织。本文采用固体可调谐激光器Cr:LiCAF作为激发光源,在360nm ~410nm 波段范围选择多种波长对结肠癌和结肠正常组织标本进行LIF测试,找出了能诱导结肠正常组织与癌变组织产生最大荧光光谱差异的最佳激发波长,并对31 组正常组织与病变组织标本进行测式,结果表明结肠正常组织与癌变组织自体荧光光谱存在明显差异。 相似文献
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针对仿生微纳导航传感器对380~520nm波长的探测要求,从Lambert定理出发,设计了一种蓝紫光探测器,并对器件的结构和工艺进行了优化分析.利用SOI硅片上薄的单晶硅层,实现了较高的蓝紫光响应度,同时抑制了可见光中的长波和近红外光的响应度.当SOI硅片的器件层厚度为3μm时,450nm波长的响应度为0.348 A/W,900nm波长的响应度为0.054 A/W.数值计算和理论分析表明,设计的器件是一种高性能的蓝紫光敏感光电探测器.Abstract: According to the requirement of detecting the wavelength of 380~520 nm of the bionic micro-nano navigation sensor,a blue/violet sensitive silicon photodetector based on Lambert Law was proposed.The structure and process were optimized and analyzed.By taking advantage of the thin layer of SOI wafer,the detector repressed the responsivity of longer wavelength in the visible and near-infrared range while maintaining a high blue/violet responsivity.When the thickness of the device layer of the SOI wafer was 5 μm,the responsivities of λ= 450 nm and λ= 900 nm were 0.348 A/W and 0.054 A/W respectively.Numerical calculation and theoretical analysis show that the designed device is a high performance photodetector with high blue/violet responsivity. 相似文献
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泡生法生长KYb(WO4)2晶体及其结构与光谱性能 总被引:5,自引:3,他引:5
对顶部籽晶提拉法(TSSG)进行了改进,采用泡生法生长KYb(WO4)2晶体(简称KYbW),以0.05℃/h的速率降温生长,转速5~10 r/min,生长周期10~15 d,降温速率15~20℃/h。研究了KYbW晶体的结构,给出了两种晶胞的选取方法。通过晶体的X射线衍射(XRD)谱,计算出晶体的两种晶格常数分别为a=1.061 nm,b=1.029 nm,c=0.749 nm,β=130.65°;a′=0.807 nm,b′=1.029 nm,c′=0.749 nm,β′=94.34°。测得晶体的吸收光谱,结果表明其吸收带在920~1000 nm,计算出主吸收峰981 nm处的吸收截面为11.12×10-20cm2。测得晶体的荧光光谱,结果表明KYbW晶体在1007 nm和1037 nm附近都有较强的发射峰,主峰1037 nm处的发射线宽(FWHM)达13 nm,因此KYbW晶体可作为可调谐激光增益介质,计算出1037 nm处的受激发射截面积eσm=3.4×10-20cm2。 相似文献
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利用日本岛津RF-5301PC荧光分光光度计,对枸杞子粉末中的维生素B2(VB2)直接进行荧光光谱检测,采集的光谱范围在530~650nm波段。采用MATLAB数值微分和高斯函数拟合方法,得到在激发波长为480nm、峰值波长为563nm时的荧光光谱信息;通过数值积分和线性拟合,得到荧光强度沿波长的积分与VB2含量呈良好的线性关系,其相关系数为0.998 43。实验测定枸杞粉末VB2含量为1.28±0.17μg/g,并阐释了枸杞子粉末荧光光谱红移的原因。研究表明:这种固体粉末荧光光谱检测方法具有快速、直接、简单和环保等优点,对药品成份检测、食品安全监测等领域的应用具有重要的参考价值。 相似文献