STUDIES ON PRESERVATION OF SUGARCANE JUICE

ABSTRACT

The variety CoP 92226 was selected for preparing sugarcane juice beverage on the basis of yield and sensory attributes from eight promising varieties of sugarcane. Sugarcane juice beverage samples were prepared by pasteurizing the sugarcane juice at 70°C for 10 minutes and adding citric acid (40 mg/100 ml), ascorbic acid (40 mg/100 ml) and potassium metabisulphite (150 ppm). Samples of sugarcane juice beverage were stored at room (30±5°C) and refrigeration (4±2°C) temperature in pre-sterilized glass bottles and analyzed for physico-chemical, microbiological and sensory attributes at every 15 days interval for 90 days. The pH, total soluble solids and total sugars decreased, whereas, titratable acidity and reducing sugars increased significantly (P<0.01) during storage. An appreciable increase in total plate counts and yeast and mold counts were observed, however, no coliforms, were detected in sugarcane juice beverage during storage. The changes in different attributes were significantly (P<0.01) higher at room temperature as compared to refrigeration temperature. The sugarcane juice beverage having citric acid and potassium metabisulphite showed minimum changes in sensory qualities during storage, both at room and refrigeration temperature. An acceptable quality beverage of sugarcane juice with satisfactory storage stability for 90 days at room as well as refrigeration temperature could be prepared.     

Compositional changes during guava fruit ripening

Compositional changes of fruit pulp and peel during ripening of white- and pink-fleshed guava fruits were studied. The white and pink guava fruits exhibited a typical climacteric pattern of respiration. Fruit tissue firmness decreased progressively, in a similar manner, in both guava fruit types. Total soluble solids (TSS) and total sugars increased in pulp and peel of both guava types with decrease in flesh firmness. More increase in total sugars was observed after the climacteric peak of respiration. Reducing sugars and titratable acidity increased up to the full-ripe stage and then decreased. Ascorbic acid and phenolic compounds decreased continuously during ripening of the two types. The peel showed higher values of ascorbic acid, total protein and phenolic compounds than the plup. The white-fleshed guavas had higher levels of TSS, total sugars, reducing sugars. titratable acidity, phenolic compounds and ascorbic acid content then the pink-fleshed fruits.     

Physicochemical Characterization of Pure Persimmon Juice: Nutritional Quality and Food Acceptability

Technological process for production of non‐astringent persimmon (Diospyros kaki Thunb. cv. “Rojo Brillante”) juice was described. The degree of fruit ripening expressed as color index (CI) varied between 12.37 and 16.33. Persimmon juice was characterized by determining physicochemical quality parameters as yield, total soluble solids (TSS), pH, titratable acidity (TA), organic acids, and main sugars. A thermal treatment of 90 ºC for 10 s was effective in controlling naturally occurring microorganisms for at least 105 d of storage without significantly affecting production of soluble brown pigments (BPs) and 5‐hydroxymethyl furfural (5‐HMF), total phenolic compounds (TPC), antioxidant capacity and acceptability of juice by panelists. Storage time affected all and each of the above parameters, reducing BPs, TPC and antioxidant capacity but increasing 5‐HMF content. Refrigerated storage enhanced the acceptability of the juices. This information may be used by the juice industry as a starting point for production of pure persimmon juices.     

Degradation of quality attributes of sweetened Julie and Ogbomoso mango juices during storage

Changes in the quality attributes of sweetened Julie and Ogbomoso mango juices were investigated. Sweetened mango juices were processed, bottled and stored at 25 °C for 12 weeks. The titratable acidity, pH, total solids, ash, soluble solids and ascorbic acid contents were evaluated immediately after processing and subsequently at 2-week intervals. The L*, a* and b* (HunterLab) parameters were determined to evaluate colour changes due to non-enzymatic browning.Generally, the quality attributes of the sweetened mango juices decreased during storage. The titratable acidity, pH, total solids, ash, soluble solids and ascorbic acid contents decreased during storage. After 12 weeks of storage, the percentage ascorbic acid loss of sweetened Julie and Ogbomoso mango juices was 16.25 and 16.67%, respectively. Browning index, chroma and b* values increased during storage. Colour changes in sweetened mango juices were mainly due to b* value and chroma.     

Synergistic impact of sonication and high hydrostatic pressure on microbial and enzymatic inactivation of apple juice

The combination of innovative, non-thermal technologies for the production of safe and quality fruit juices is a recent trend in food processing. The purpose of this study was to evaluate the effects of combined treatment of ultrasound (US) and high hydrostatic pressure (HHP) on enzymes (polyphenolase, peroxidase and pectinmethylesterase), microorganisms (total plate counts, yeasts and molds) and phenolic compounds (total phenols, flavonoids and flavonols) of apple juice. Moreover, its effects on ascorbic acid, antioxidant capacity and DPPH free radical scavenging activity, color values, pH, soluble solids and titratable acidity were investigated. Fresh apple juice was treated with US (25 kHz and 70% amplitude) at 20 °C for 60 min with subsequent HHP treatment at 250, 350 and 450 MPa for 10 min at room temperature. The results revealed that the combined US-HHP450 treatment caused highest inactivation of enzymes with complete inactivation of total plate counts, yeasts and molds. It also significantly improved the phenolic compounds, ascorbic acid, antioxidant capacity, DPPH free radical scavenging activity and color values. The present results suggest that the combination of US and HHP can act as a potential hurdle to produce safe and high quality apple juice with reduced enzymes and microbial activity and improved nutrition.     

Plum Juice Quality Affected by Enzyme Treatment and Fining

Juice from six plum (Primus domestica L.) cultivars, Au Red, Abundance, Pobeda, Shiro, Peach Plum and Early Golden, was extracted using 0.2% Clarex® L pectinase. The juice was processed as high temperature-short time (HTST)-unfined juice, fined juice or HTST-fined juice, and analyzed for yield, clarity, pectin content, soluble solids, pH, titratable acidity, color, sugars, total anthocyanins and total phenolics. Pectinase treatment resulted in 41–214% increase in juice yields, with highest increase for Au Red. Enzyme-extracted juice averaged 54% lower pectin than controls, and fining further reduced pectin. Enzyme treatment also improved juice soluble solids and color. A consumer type sensory panel indicated juice from Abundance, Pobeda and Peach Plum had best flavor and acceptability.     

Time and Temperature Effect on Stability of Moroccan Processed Orange Juice during Storage

The loss of quality of processed pasteurized orange juice stored at 4°C, 22.5°C, 35°C, and 45°C for up to 14 wks was evaluated. The results showed that parameters such as pH, total solids, titratable acidity, formal index, and total sugars did not significantly change during storage at all temperatures. However, a major change was observed for ascorbic acid content, reducing sugars and furfural production, except for storage temperature at 4°C. Ascorbic acid degradation, sucrose hydrolysis, and furfural build-up followed pseudo-zero order reaction kinetics. The minimal change of formol number and total sugars suggested that nonenzymatic browning was mainly due to ascorbic acid degradation. Fufurfal formation during storage was found to be much higher than that reported in the literature.     

COLOR QUALITY OF FROZEN STRAWBERRIES: EFFECT OF ANTHOCYANIN, pH, TOTAL ACIDITY AND ASCORBIC ACID VARIABILITY

SUMMARY— 40 lots of strawberries, consisting of 13 different selections and 5 varieties, were packed as frozen, sliced, sugared (4:1) fruit. Total anthocyanin content, the relative amounts of pelargonidin-3-glucoside and cyanidin-3-glucoside, ascorbic acid, pH, total acidity, soluble solids and Gardner L, a, b. determinations were made. After 9 months of storage, panel evaluation of the color quality and Gardner L, a and b determinations were taken. Results of correlation analyses indicated that pH was the only objective measurement having a high correlation with color quality. Neither ascorbic acid, total acidity nor soluble solids had significant correlations with color quality. From regression equation values, it was calculated that berries should have a pH of 3.51 or lower to have acceptable color after freezing. Anthocyanin content should be in the approximate range 450–700 μg/g to have acceptable color quality.     

Identification of Bioactive Compounds From <Emphasis Type="Italic">Vitis labrusca</Emphasis> L. Variety Concord Grape Juice Treated With Commercial Enzymes: Improved Yield and Quality Parameters

In this work, the effects of eight different enzymatic preparations were compared in terms of the extraction of bioactive compounds as well as the yield and quality of grape juice from Vitis labrusca L. variety Concord. Juices were prepared by enzymatic treatment and the used preparations were characterized through five enzymatic activities: total pectinase (PE), polygalacturonase (PG), pectinlyase (PL), pectin methyl esterase (PME), and cellulase (CE). Quality parameters such as color, total soluble solids (°Brix), reducing sugars, titratable acidity, pH, and antioxidant capacity were determined for all samples. Phenolic compounds and anthocyanins were identified and quantified by HPLC-DAD-MS. Juice yield was improved by preparations with high activities of PG, PME, and PL, up to 9 % higher than the control, when used Pectinex Ultra Clear. For the extraction of bioactive compounds, the highest values were found for preparations with high CE activity. Regarding the phenolic compounds, 15 compounds were identified. The content of caftaric acid and total anthocyanins was improved up to 300 and 60 %, respectively, using Lallzyme Beta. It was possible to understand the role of each enzyme in the extraction of yield, quality parameters, and bioactive compounds. Pectinex Ultra Clear was the preparation that provided the highest yield and Lallzyme Beta the highest bioactive compounds concentration. For a good quality juice and high yields for the industry, it is important to have a good balance of pectinases and cellulases.     

A comparative study of composition and postharvest performance of organically and conventionally grown kiwifruits

Postharvest performance of organic and conventional ‘Hayward’ kiwifruits grown on the same farm in Marysville, California, and harvested at the same maturity stage were compared in this study. Quality parameters monitored included morphological (shape index) and physical (peel characteristics) attributes of the initial samples. Maturity indices (CO₂ and C₂H₄ production, firmness, color, soluble solids content and acidity) and content of compounds associated with flavor and nutritional quality (minerals, sugars and organic acids, ascorbic acid, total phenolics, and antioxidant activity) were determined at 0, 35, 72, 90 and 120 days of storage at 0°C, and after 1 week of shelf‐life simulation at 20°C, after each storage duration. Organically and conventionally grown kiwifruits had similar soluble solids content at harvest, but conventional kiwifruits had a higher firmness and L* value, and a lower hue angle and chromaticity, resulting in a lighter green color when compared with the organic kiwifruits. These differences were maintained for all the storage durations, with the soluble solids content increasing more in conventionally grown kiwifruits. The two production systems resulted in different morphological attributes since organic kiwifruits exhibited a larger total and columella area, smaller flesh area, more spherical shape, and thicker skin compared to conventional kiwifruits. All the main mineral constituents were more concentrated in organic kiwifruits, which also had higher levels of ascorbic acid and total phenol content, resulting in a higher antioxidant activity. Sugars and organic acids composition was not affected by the production system. Copyright © 2007 Society of Chemical Industry     

Effect of nitric oxide on pericarp browning of harvested longan fruit in relation to phenolic metabolism

The effects of nitric oxide (NO) on enzymatic browning of harvested longan fruit in relation to phenolic metabolisms were investigated. Fruits were dipped for 5 min in 1 mM sodium nitroprusside (SNP), a nitric oxide donor, then packed in 0.03 mm thick polyethylene bags, and finally stored for 6 days at 28 °C. Changes in pericarp browning and pulp breakdown were evaluated, while total phenol content, activities of phenolic-associated enzymes, polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonia lyase (PAL), and concentrations of total soluble solids, titratable acidity and ascorbic acid were measured. SNP treatment delayed pericarp browning, inhibited activities of PPO, POD and PAL and maintained a high total phenol content of longan fruit during storage. Furthermore, NO showed a significant inhibition of the in vitro activities of PPO and POD, indicating that the beneficial effect of NO was direct. Moreover, application of NO resulted in a lower pulp breakdown and maintained relatively high levels of total soluble solids and ascorbic acid.     

蟠桃品种用于加工鲜榨汁的适宜性评价

为研究不同蟠桃品种的鲜榨汁加工适宜性,本实验对北京平谷地区的7 个蟠桃品种的理化与营养指标（pH值、总酚含量、抗坏血酸含量、蛋白质含量、可滴定酸含量、可溶性固形物含量（soluble solids content,SSC）、固酸比、果胶含量）和加工品质指标（单果质量、L*、a*、b*值、褐变度、出汁率、黏度）进行了测定,并从色泽、气味和风味三方面进行感官评价。通过相关性分析、主成分分析和聚类分析法筛选出了SSC、出汁率、果胶含量、a*值和蛋白质含量5 个核心品质评价指标。通过层次分析法得出：SSC和出汁率的权重值为0.32,果胶的权重值为0.18,a*值和蛋白质的权重值分别为0.11和0.07。对7 个蟠桃品种的鲜榨汁品质综合评价表明：瑞蟠21的鲜榨汁品质最好,其次为瑞蟠20、瑞蟠4和瑞蟠19,而巨蟠、瑞蟠2和瑞蟠3的鲜榨汁品质较差。     

EFFECT OF OXYGEN CONCENTRATION ON THE BIOCHEMICAL AND CHEMICAL CHANGES OF STORED LONGAN FRUIT

ABSTRACT

Longan fruits were stored for 6 days in atmosphere of 5, 21 (air) or 60% O₂ (balance N₂) at 28C and 90–95% relative humidity to examine effects of low and high O₂ concentration on enzymatic browning and quality attributes of the fruit. Changes in pericarp browning, pulp breakdown, disease development, total phenol content, activities of phenol metabolism‐associated enzymes, relative leakage rate, α,α‐diphenyl‐β‐picrylhydrazy (DPPH) radical scavenging activity, and contents of total soluble solids, titratable acidity and ascorbic acid were evaluated. Storage of fruit in a 5% O₂ atmosphere markedly delayed pericarp browning in association with maintenance of high total phenolic content and reduced activities of polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonia lyase. Moreover, the fruit stored in a 5% O₂ atmosphere exhibited a lower relative leakage rate and higher DPPH radical scavenging activity than fruit stored in air. This presumably was beneficial in maintaining compartmentation of enzymes and substrates, and thus, reducing pericarp browning. Pulp breakdown and disease development were also reduced by exposure to a 5% oxygenatmosphere. On the contrary, exposure of longan fruit to a 60% O₂ atmosphere accelerated pericarp browning, pulp breakdown and decay development. PPO and POD activities and relative leakage rate were similar for control and 60% O₂‐treated fruit after 4 and 6 days of storage. Furthermore, treatment with 60% O₂ significantly decreased the phenolic content and DPPH scavenging activity of fruit. In addition, exposure to 5 or 60% O₂ resulted in a higher level of total soluble solids, but a lower level of ascorbic acid of longan fruit flesh. In conclusion, exposure to a 5% O₂ atmosphere showed great potential to reduce pericarp browning and extend shelf life of longan fruit.

PRACTICAL APPLICATIONS

Pericarp browning and pulp breakdown are the major causes of deterioration in postharvest longan. Conventional controlled atmosphere with low O₂ and high CO₂ is effective in maintaining quality and extending shelf life of fruits and vegetables, including inhibition of tissue browning. In this study, 5%‐controlled atmosphere reduced significantly pericarp browning, pulp breakdown and rot development. It could potentially be useful as a postharvest technology of longan fruit for reducing or replacing the use of chemicals such as SO₂ and fungicides, but it requires further investigation.     

COMPOSITIONAL ANALYSIS OF FOUR RED RASPBERRY CULTIVARS GROWN IN MAINE

The compositional characteristics (total soluble solids, pH, titratable acidity, moisture, pectin, reducing sugars, nonreducing sugars and total sugars) of four raspberry cultivars (‘Boyne’, ‘Festival’, ‘Killarney’ and ‘Prestige’) grown in Maine were evaluated during three consecutive years. Significant differences were found among these cultivars. In different growing seasons there were also significant effects on these characteristics in the same cultivar.     

Novel approaches to reduce brown pigment formation and color changes in thermal pasteurized tomato juice

The effects of two heat processes mild-temperature pasteurization (MTP: 75 °C, 23 s) and high-temperature pasteurization (HTP: 92 °C, 5 s) on undesirable brown color development and some quality attributes (pH, soluble solids content, and color parameters) of pasteurized tomato juice were evaluated and compared during 94 days of storage at 5 °C. Although both heat treatments combined with refrigeration prevented microbial growth for 64 days, the shelf life regarding acceptable microbial load of HTP-treated sample was higher than 94 days. Both processes produced lesser brown color formation in tomato juices than the commonly adopted thermal treatments although the MTP processing provides better color preservation than the HTP one. No effect of heat treatments on soluble solids content was observed while the HTP processing slightly increased the acidity of pasteurized tomato juice. The good relationship obtained between brown color and total color difference (TCD) suggests that TCD can be used to evaluate the visual color deterioration and browning in thermally processed tomato juice throughout refrigerated storage. Results presented in this study showed that the combination of a mild heat treatment plus refrigeration may help to ensure the microbiological safety and the nutritional value of pasteurized tomato juices.     

EFFECT OF SOLUBLE SOLIDS AND TEMPERATURE ON ASCORBIC ACID DEGRADATION IN LEMON JUICE STORED IN GLASS BOTTLES

Lemon juice at concentrations of 9°, 20°, 30°, 40° and 50°Brix was stored at 10°, 20° and 36°C for 16 weeks and sampled regularly for total soluble solids (TSS), pH, titratable acidity and ascorbic acid. No significant differences were found in the first two of these factors as a function of storage time. There was a small but significant decrease in citric acid concentration over 16 weeks. Ascorbic acid loss was greater at higher temperatures; at a constant temperature, the loss was smaller as TSS increased. Ascorbic acid degradation data fitted zero-, first- and second-order models equally well at all five TSS. Rate constants in 9°Brix juice were significantly higher than those for the other four concentrations at all three temperatures. E_a values of 47.8 and 24.1 kJ mol^?1 were calculated for ascorbic acid degradation in 9° and 20°Brix juices. The effect of temperature far outweighed the effect of TSS on ascorbic acid degradation. Over the 16-week storage period, maximum retention of ascorbic acid (95.7%) was obtained in the 50°Brix lemon juice concentrate stored at 10°C.     

QUALITY OF APPLE FRUIT IN RIGID FILM CONTAINERS

The storage quality of‘Delicious’ apples individually packaged in rigid film containers was investigated. Objective quality attributes (internal and external color, firmness, soluble solids and titratable acidity) were measured as a function of storage time with and without packaging in a rigid container. Overall firmness, acidity and soluble solids content decreased over a 70-day storage period. Significant effects of rigid film packaging and storage included: superior skin color, decreased weight loss, slight decrease in soluble solids content and increased firmness loss during ripening when compared to control fruit. Upon examination of the atmosphere surrounding individual fruit in these containers, increased levels of carbon dioxide and ethylene were found. Conversely, packaged fruit contained significantly less internal carbon dioxide than control samples. It was concluded that the atmosphere modified by the rigid film container hastened ripening of‘Delicious’ apples, thus deteriorating eating quality. An adequately ventilated packaged would probably alleviate this condition.     

Preservation of mango juice by freezing and canning

In this work the preservation of mango juice by freezing and canning was compared. The following results were obtained: The loss of ascorbic acid during the preparation of canned mango juice is mainly due to the processing steps other than retorting which had the least harmful effect. The retention of ascorbic acid and carotene after canning and freezing was nearly the same. Both freezing and canning caused no marked changes in the pectin content. The inactivation of the pectin methylesterase was more obvious after canning than after freezing. Reducing sugars slightly increased after freezing and canning, while the acidity and pH showed no appreciable changes. The chemical constituents except the sugars of the commercial samples were lower than those of the experimental samples. During storage, the percentage retention of ascorbic acid and carotene were lower when pasteurization was not included, and in canned samples they were higher in the experimental juice than in the market samples. Pectin content showed no changes in all cases studied. The activity of pectin methylesterase slightly decreased in frozen samples, while it was a small increase in case of canned juices. During storage the microbial count of frozen and canned samples decreased when storage progressed. The addition of water and sugar during manufacturing of commercial samples did not help to obtain a product with good storage stability. There was a significant difference between the characteristics of frozen pasteurized and non pasteurized mango juice. It seems very important to pasteurize mango juice before storage in a frozen form.     

超高压处理对猕猴桃汁品质的影响

本实验主要探讨了热敏性纯猕猴桃汁经100～500 MPa超高压处理后品质的变化情况.考察的指标有果汁色泽、黏度、pH、单宁、总酸、果胶、总VC、蛋白质以及可溶性固形物,并与未经超高压处理的果汁对照品进行了比较.实验结果表明,超高压处理能较好保持果汁原有的品质和风味,但有些现象还需要进一步从理论上探讨.     

QUALITY CHANGES IN APPLE JUICE AS RELATED TO NONTHERMAL PROCESSING

Fresh apple juice was preserved using ultrafiltration and high voltage pulsed electric fields as nonthermal preservation techniques. Changes in quality attributes such as soluble solids, pH, acidity and color were verified for both processes. Changes were tested in terms of membrane pore, transmembrane pressure and recovery percentage for the ultrafiltration treatments, as well as in terms of electric field strength and number of pulses for the pulsed electric fields experiments. Among the quality attributes investigated, soluble solids, pH and acid content did not show significant differences, while color was significantly different for both nonthermal techniques. For the ultrafilteredjuice, a browning trend was observed, whereas for the pulsed electric treated juice an opposite, lightening, tendency was present.