白玉银桦组培快繁技术研究

[目的]研究白玉银桦(Grevillea baileyana)的组培快繁技术,以提高繁殖速率和生根率,建立一套高效的再生体系.[方法]以白玉银桦半木质化枝条为外植体,对适宜的丛芽诱导、增殖、生根的培养基进行了系统研究.[结果]最适启动培养基为:MS+6-BA 1.0mg/L+NAA0.1 mg/L+蔗糖30 g/L+琼脂粉6 g/L;最佳增殖培养基为:MS+6-BA1.0 mg/L+KT 0.2 mg/L+NAA 0.3 mg/L+蔗糖30g/L+琼脂粉6 g/L,增殖系数达3.6以上;适宜的生根培养基为:1/2MS+NAA 0.3 mg/L+蔗糖20 g/L+琼脂粉6 g/L,生根率达到90%以上,生根苗平均根数3.0条.[结论]该研究为解决目前白玉银桦种苗的供需矛盾,开辟了一条切实有效的方法和途径,具有重要的理论价值和现实意义.     

速生优良杉木组培继代及生根培养研究

[目的]研究培养基、6-BA、IBA、NAA等4个因子对杉木(Cunninghamia lanceolata)组培苗芽增殖的影响和蔗糖、IBA、NAA、ABT6<'#>等4个因子对组培苗生根培养的影响,寻求最适杉木组培苗芽增殖和生根的培养基配方,为杉木组培快繁提供一些有益的参考.[方法]应用正交设计法研究培养基、6-BA、IBA、NAA等4个因子对杉木组培苗芽增殖的影响和蔗糖、IBA、NAA、ABT6<'#>等4个因子对组培苗生根培养的影响.[结果]最佳的继代增殖培养基是1/3MS+蔗糖30 g/L+6-BA 0.7 mg/L+IBA 0.5 mg/L,最适于生根的培养基是1/2MS+蔗糖30 g/L+IBA 1.2 mg/L+NAA 0.4 mg/L.[结论]该研究为杉木组织培养中选择合适的培养基提供了科学依据.     

铁皮石斛原球茎增殖培养基配方的优化

[目的]获得铁皮石斛(Dendrobium candidum Wall.ex Lindl.)原球茎组织培养的最佳培养基配方.[方法]用正交试验筛选出6-BA、NAA、KT 最佳的浓度配比,并考察各因素对铁皮石斛原球茎增殖率的影响.[结果]铁皮石斛原球茎增殖的最佳培养基配方为1/2MS+2.0 ms/L 6-BA+0.5 ms/L NAA+1.0 ms/L KT.[结论]该研究为工业化生产铁皮石斛原球茎及其有效成分,推进铁皮石斛工厂化育苗奠定了基础.     

黑刺李的组织培养与快速繁殖

[目的]为黑刺李品种改良和工厂化繁育提供理论依据.[方法]以黑刺李幼嫩叶片为外植体,MS为基本培养基,分别对其进行启动、增殖及生根培养,建立黑刺李离体培养再生体系.[结果]外植体在启动培养基MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+2,4-D 0.5 mg/L上培养15 d后边缘膨大,30 d 后形成愈伤组织,60 d 后形成丛生芽;带丛生芽的愈伤组织块在增殖培养基MS+6-BA 0.3 mg/L+NAA 0.1 mg/L上培养28 d 后陆续分化出3～5个丛芽;将增殖培养获得的2 cm以上的无根小苗接种到生根培养基1/2MS+IBA 0.4 mg/L+蔗糖 7.0 g/L上培养15 d后开始生根,25 d后生根率达75%;将试管苗移栽到基质(草炭∶蛭石∶珍珠岩=1∶1∶2)上养护,30 d 后成活率达80%以上.[结论]该研究建立的黑刺李离体培养再生体系稳定性好、增殖速度快.     

绿巨人组织培养及快速繁殖技术

[目的]研究绿巨人的组织培养方法, 建立其快速繁殖技术体系,为发展其产业化生产提供技术支撑.[方法]以绿巨人嫩枝顶芽和腋芽为外植体, 选择不同培养基和激素配比,进行诱导培养、继代培养、温室生根锻炼和大田移栽试验,初步建立绿巨人组织培养快速繁殖体系.[结果]适宜的愈伤组织诱导培养基为:MS+2.0mg/L6-BA+0.05 mg/L NAA+0.25 mg/L2,4-D+蔗糖30%;继代增殖培养基为:MS+5.0mg/L 6-BA+0.02 mg/L NAA+蔗糖30%;生根培养基为:MS+0.5 mg/L.IBA 在建立的快繁体系下辅以合适的外界条件,绿巨人炼苗移栽的成活率在95%以上.[结论]研究为绿巨人组织培养及快速繁殖提供了技术支撑.     

绿巨人的生根培养研究

[目的]建立绿巨人快速生根体系.[方法]以绿巨人无菌苗为外植体,对绿巨人生根最适培养条件进行研究.[结果]绿巨人最适生根条件为1/2MS+0.5 mg/L NAA+8.0 mg/L AC.此条件下,20 d和30 d生根率分别达到88.2%和100.0%.[结论]适当浓度的活性炭和蔗糖利于绿巨人快速生根.     

春石斛和铁皮石斛试管苗壮苗生根条件的优化

以离体保存5 a的春石斛和铁皮石斛原球茎为材料,采用正交试验对其试管苗壮苗生根的条件进行优化,从鲜重、假鳞茎、颜色、株高和根系生长等方面对植株进行综合评定.结果表明:适宜春石斛壮苗生根的培养基为1/2MS+20 g.L-1白糖+50 g·L-1香蕉匀浆物;适宜铁皮石斛壮苗生根的培养基为1/2MS+10 g·L-1白糖.     

邢台野生酸枣组织培养研究

[目的]建立邢台野生酸枣组织培养快繁体系,为野生酸枣的规模化开发种植奠定基础.[方法]以邢台野生酸枣当年生枝条为外植体进行组培快繁研究、并探讨合适的培养基种类.[结果]邢台野生酸枣组织培养外植体最佳消毒方法是浓度70%乙醇消毒30s,0.1%升汞消毒10min.其最佳培养基组合为:诱导培养基1/2MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;增殖培养基MS+6-BA 1.0或1.5 mg/L+NAA0.2 mg/L;生根培养基1/2MS+6-BA0.5 mgL.[结论]初步研究出邢台野生酸枣组织培养方法,并筛选出合适的诱导、增殖及生根培养基.     

红掌工厂化育苗关键技术研究

[目的]研究红掌工厂化育苗的关键技术.[方法]以红掌盆花和切花品种的叶片、叶柄、花苞片和花苞柱头为外植体,进行组培养快速繁殖技术研究,探讨外植体的选择和消毒条件以及不同培养基对红掌愈伤组织的诱导、增殖和生根影响.[结果]叶柄为最佳外植体,愈伤诱导率最高,达78.3%;外植体采用0.1%HgCl<,2>消毒8 min的效果最好;粉冠军和MIDORI的最佳愈伤组织诱导培养基分别为1/2MS+6-BA 1.50 mg/L+2,4-D0.50 mg/L+NAA0.10 mg/L和1/2 MS+6-BA 1.50mg/L+KT 1.00 mg/L+2,4-D0.10 mg/L;最佳愈伤组织继代培养基分别为MS+6-BA 1.00 mg/L+NAA 0.50 mg/L+2,4-D0.30 mg/L和MS+6-BA 0.50 mg/L+KT0.50 mg/L+NAA0.10mg/L,添加10%的椰子汁或香蕉浆有利于增殖和丛芽生长,增殖率最高达2.83倍;最佳生根培养基为1/2 MS+NAA 0.10 mg/L+IAA0.10mg/L,生根率达100%.[结论]该结果为红掌工厂化育苗体系的建立奠定了基础,对确保红掌产业的发展具有重要意义.     

短梗胡枝子的组织培养研究

[目的]研究短梗胡枝子(Lespedeza cyrtobotrya)的组织培养.[方法]以短梗胡枝子种子为材料,对其组织培养进行了系统研究.[结果]2.1%次氯酸钠灭菌8 min,发芽率较高,且污染率为0;在初代培养中,基础培养基为Ms,6-BA对分化系数影响达到显著水平,适宜的增殖培养基为MS+1.00 mg/L 6-BA+0.10 mg/L NAA+0.01 mg/L2,4-D,分化系数可达6.69;在继代培养中,适宜的培养基为MS+1.00 mg/L 6-BA+0.05 mg/L 2,4-D;在生根培养中,IBA浓度为0.50 mg/L时,生根率和平均生根数都最佳.[结论]为进一步开展胡枝子基因工程遗传改良提供了理论依据.     

转炉炉壳热应力分析

根据实际转炉建立实体模型。以有限元为手段,考虑了炉衬和炉壳材料的物性参数随温度变化的特点和炉衬与炉壳之间膨胀间隙,计算了转炉炉壳在温度载荷和炉衬膨胀压力同时作用下的热应力。所得结果可为转炉炉壳设计提供依据。     

The effect of amount of possession of the trait of hostility on accuracy of perception of hostility in others.

Some theories of personality hold that an individual's perception of others is influenced by his own personality characteristics; more specifically, that a trait of low social value will be projected more into others, and that insight into the possession of these characteristics reduces this distortion. These 2 hypotheses were tested by having a group of college students rate each other (including themselves) on degree of friendliness or hostility. Agreement with group rating about self yielded the insight score. In general, the hypotheses were supported; the "friendly" Ss were most accurate in their perception of others, and insight was positively associated with accurate perception. From Psyc Abstracts 36:04:4HL16M. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of rates of infection of two methods of emergency ventricular drainage

The rates of infection of two methods of external ventricular drainage in use at Atkinson Morley's Hospital--namely, (a) percutaneous drainage with Rickham reservoirs and (b) tunnelled ventriculostomies--were compared in this retrospective review. Percutaneous drainage of CSF with Rickham reservoirs was associated with a 27% rate of infection as identified by positive microbiological cultures; tunnelled ventriculostomy catheters had a 10% infection rate. The difference in the infection rate between the two methods was statistically significant (P < 0.015). Other variables examined, including the age and sex of the patients and the reasons for ventricular drainage, were not associated with an increased rate of infection. Most infections from either method were caused by a coagulase negative staphylococcus. The average duration of ventricular drainage before identification of positive cultures was 5.7 days for Rickham reservoirs and 6.0 days for ventriculostomies.     

浅析《野草》的版画特征

鲁迅先生一生热爱美术作品,对版画中木刻兴趣尤甚.<野草>作为其"写心之作",亦呈现出一些与木刻版画一致的审美特征:一是具有木刻版画的色彩特征,多对比、多冷色,有阴郁的美感;二是具有木刻版画的力度特征,环境描写、人物刻画处处刀锋毕现,遒劲有力,有一种"力之美".     

四辊破碎机车刀架的改造

针对四辊破碎机车刀架车削中存在的几个问题,详细介绍了电动车刀架的结构和工作原理,建立主要设计参数的数学表达式,并进行适当的分析。改造后,这种电动车刀架运行平稳、可靠、方便、省力,取得了预期的效果。     

焦炭热性能指标意义浅析

通过对焦炭在高炉内的历程及其变化规律的剖析,阐述焦炭热性能指标的意义,形象地把焦炭在高炉内的损耗分为三部分(M1,M2,M3)。指出M2对焦比及焦炭价格有一定的指导作用。     

Results of treatment of cancer of the cardia

Cancer of the cardia is traditionally discussed with cardiac cancer of the lower portion of the esophagus and upper gastric cancer invading the esophagus, and the specific characteristics of cancer of the cardia have never been clearly defined. We reviewed the outcome of 172 patients with adenocarcinoma of the cardia who had undergone radical surgery between 1949 and 1994 in the Division of Gastrointestinal Surgery of the Cancer Institute Hospital. The centers of the tumors were located within 2 cm above and below the boundary between the esophagus and the stomach, and their longitudinal diameter was less than 8 cm. We divided the patients into an early period (1949-1979; n = 79) and a late period (1980-1994; n = 93), and focused on the historical transition. There were no differences in patient gender or histology between the two periods. However, the late period was associated with fewer cases of esophageal invasion and shorter longitudinal diameter, as the age of the population advanced. The number of advanced cancers such as the localized and infiltrative type had decreased, and early cancer and early clinical stage had become more common, but despite the fact that the number of early cancers had increased, extended dissection, such as thoracic and paraaortic lymph node dissection, was performed more frequently. By clinical stage, the long-term outcome markedly improved in Stage I patient in the late period, and tended to improve in Stage II and III patients. This appears to have been attributable to the prevention of micrometastasis by extended dissection, although the number of early cancers is another major potential cause. There were no differences in the outcome of Stage IV patients between the two periods, and further advances in multimodality therapy must be awaited. The range of resection is basically proximal gastrectomy, and if there are adequate indications, the prognosis is favorable. In view of the status of lymphatic flow and lymph node metastasis, and long-term results, lateroaortic lymph node dissection is important. Since the number of early cancer patients has been increasing, if intraabdominal recurrence is prevented, intrathoracic lymph node dissection will contribute greatly to the outcome of such patients.     

Comparison of the results of surgical and conservative treatment of fractures of the diaphysis of the tibia

In this paper is treated the fundamental problematic of the fracture of the diaphysis of the tibia and the therapeutic results after the conservative and operative treatment are confronted. Among 518 patients with 433 open fractures and 35 with closed fractures of the diaphysis of the tibia, successful treatment of more 2/3 was obtained in the period from 1968 to 1972. Following our casuistics and literature, whose results are similar, we find out that the average total treatment of the osteosynthesis of the fracture of the diaphysis was shortened for 2,5 months depending upon the type and the localisation of the fracture. Apolied methods of the operative and conservative and conservative treatment in relation to the velocity of the medical cure as well as to the extreme anatomical-functional results are analised. We noticed that osteosynthesis, made by the intermedullary nail and the extension of the canals or by a small AO table, by the achieved stability makes conditions of immediate motions and an early rehabilitation. Because of that it gives the best functional results.