Development of a Tunable LED-Based Colorimetric Source

A novel, spectrally tunable light-source utilizing light emitting diodes (LEDs) for radiometric, photometric, and colorimetric applications is described. The tunable source can simulate standard sources and can be used as a transfer source to propagate photometric and colorimetric scales from calibrated reference instruments to test artifacts with minimal increase in uncertainty. In this prototype source, 40 LEDs with 10 different spectral distributions were mounted onto an integrating sphere. A voltage-to-current control circuit was designed and implemented, enabling independent control of the current sent to each set of four LEDs. The LEDs have been characterized for stability and dependence on drive current. The prototype source demonstrates the feasibility of development of a spectrally tunable LED source using LEDs with up to 40 different spectral distributions. Simulations demonstrate that such a source would be able to approximate standard light-source distributions over the visible spectral range—from 380 nm to 780 nm—with deviations on the order of 2 %. The tunable LED source can also simulate spectral distributions of special sources such as discharge lamps and display monitors. With this tunable source, a test instrument can be rapidly calibrated against a variety of different source distributions tailored to the anticipated uses of the artifact. Target uncertainties for the calibration of test artifacts are less than 2 % in luminance and 0.002 in chromaticity for any source distribution.     

Fundamental characteristics of a synthesized light source for optical coherence tomography

We describe the fundamental characteristics of a synthesized light source (SLS) consisting of two low-coherence light sources to enhance the spatial resolution for optical coherence tomography (OCT). The axial resolution of OCT is given by half the coherence length of the light source. We fabricated a SLS with a coherence length of 2.3 microm and a side-lobe intensity of 45% with an intensity ratio of LED1:LED2 = 1:0.5 by combining two light sources, LED1, with a central wavelength of 691 nm and a spectral bandwidth of 99 nm, and LED2, with a central wavelength of 882 nm and a spectral bandwidth of 76 nm. The coherence length of 2.3 microm was 56% of the shorter coherence length in the two LEDs, which indicates that the axial resolution is 1.2 microm. The lateral resolution was measured at less than 4.4 microm by use of the phase-shift method and with a test pattern as a sample. The measured rough surfaces of a coin are illustrated and discussed.     

Applicability of light-emitting diodes as light sources for active differential optical absorption spectroscopy measurements

We present what is to our knowledge the first use of light-emitting diodes (LEDs) as light sources for long-path differential optical absorption spectroscopy (LP-DOAS) measurements of trace gases in the open atmosphere. Modern LEDs represent a potentially advantageous alternative to thermal light sources, in particular to xenon arc lamps, which are the most common active DOAS light sources. The radiative properties of a variety of LEDs were characterized, and parameters such as spectral shape, spectral range, spectral stability, and ways in which they can be influenced by environmental factors were analyzed. The spectra of several LEDs were found to contain Fabry-Perot etalon-induced spectral structures that interfered with the DOAS evaluation, in particular when a constant temperature was not maintained. It was shown that LEDs can be used successfully as light sources in active DOAS experiments that measure NO2 and NO3 near 450 and 630 nm, respectively. Average detection limits of 0.3 parts in 10(9) and 16 parts in 10(12) respectively, were obtained by use of a 6 km light path in the open atmosphere.     

Ligand Enabling Visible Wavelength Excitation of Europium(III) for Fluoroimmunoassays in Aqueous Micellar Solutions

Fluorescent reporters based on lanthanide ions, such as europium chelates, enable highly sensitive detection in immunoassays and other ligand binding assays. Unfortunately they normally require UV-excitation produced by a xenon flash or nitrogen laser light source. In order to use modern solid state excitation sources such as light emitting diodes (LEDs), these reporters need to be excited at wavelengths longer than 365 nm, where high-powered ultraviolet LEDs are available. A novel ligand, 9-ethyl-3,6-bis(5',5',5',4',4'-pentafluoro-1',3'-dioxopentyl)carbazole (bdc), was synthesized to efficiently excite europium(III) at wavelengths up to 450 nm in micellar solutions, and its performance was compared to a commercially available DELFIA enhancement solution. The detection limit of Eu(III) with the bdc-ligand using 365 nm excitation was determined to be 63 fM, which is 3 times lower than with the DELFIA solution. The bdc-ligand enabled sensitive detection of europium(III) ions in solution using 365 nm excitation and displayed similar sensitivity and functionality as commercially available DELFIA enhancement solution. Therefore, this novel enhancement solution might be a feasible alternative in producing time-resolved fluorescence under LED-excitation.     

Optimized birefringence changes during isolated nerve activation

Single trial, birefringence signals associated with action potentials from isolated lobster nerves were optimized with high-intensity light-emitting diodes (LEDs) and glass polarizers. The narrow spectral output of the LEDs allowed us to select specific wavelengths, increasing the effectiveness of the polarizers and minimizing the stray light in the system. The LEDs produced intensity profiles equivalent to narrowband filtered 100-W halogen light, and birefringence signals were comparable or superior in size and clarity to halogen lamp recordings. The results support a direct correlation between signal size and polarizer extinction coefficient. Increasing the sensitivity of birefringence detection through the use of LED light sources could ameliorate noninvasive brain imaging techniques that employ fast optical consequences associated with action potential propagation.     

Differential spectral responsivity measurement of photovoltaic detectors with a light-emitting-diode-based integrating sphere source

We present an experimental realization of differential spectral responsivity measurement by using a light-emitting diode (LED)-based integrating sphere source. The spectral irradiance responsivity is measured by a Lambertian-like radiation field with a diameter of 40 mm at the peak wavelengths of the 35 selectable LEDs covering a range from 280 to 1550 nm. The systematic errors and uncertainties due to lock-in detection, spatial irradiance distribution, and reflection from the test detector are experimentally corrected or considered. In addition, we implemented a numerical procedure to correct the error due to the broad spectral bandwidth of the LEDs. The overall uncertainty of the DSR measurement is evaluated to be 2.2% (k = 2) for Si detectors. To demonstrate its application, we present the measurement results of two Si photovoltaic detectors at different bias irradiance levels up to 120 mW/cm(2).     

Temperature estimation of high-power light emitting diode street lamp by a multi-chip analytical solution

Light emitting diodes (LEDs) are now widely used in many fields including traffic lights, vehicle backlights and liquid crystal display (LCD) displays because of their long life, good illumination efficiency and low energy consumption. At present, LEDs are increasingly replacing the traditional lighting and are being used in general illumination such as the street lamp. For the high-power LED street lamps, good light extraction is the most important thing, but low junction temperature of the LED modules is also critical for achieving a long lifetime and a high optical efficiency. Actually, there have been many reports about early failures of street lamps, called dead lamps that have been regarded as a barrier in the public and administration acceptance of LED street lamps. Therefore temperature estimation is always a crucial issue for LED product development. A multi-chip spreading thermal resistance model was applied to estimate the temperature distribution of LED street lamp. The experiment was first done to obtain temperatures of several locations in a prototype LED street lamp. Then the multi-chip spreading resistance model was established to calculate the full temperature distribution. Comparison between the model calculation and experimental measurement showed a good agreement, which demonstrates that the present model can be used in engineering design to estimate the temperature distribution of high-power LED street lamps.     

Development of a very fast spectral response measurement system for analysis of hydrogenated amorphous silicon solar cells and modules

An important requirement for a very fast spectral response measurement system is the simultaneous illumination of the solar cell at multiple well defined wavelengths. Nowadays this can be done by means of light emitting diodes (LEDs) available for a multitude of wavelengths. For the purpose to measure the spectral response (SR) of amorphous silicon solar cells a detailed characterization of LEDs emitting in the wavelength range from 300 nm to 800 nm was performed. In the here developed equipment the LED illumination is modulated in the frequency range from 100 Hz to 200 Hz and the current generated by each LED is analyzed by a Fast Fourier Transform (FFT) to determine the current component corresponding to each wavelength. The equipment provides a signal to noise ratio of 2–4 orders of magnitude for individual wavelengths resulting in a precise measurement of the SR over the whole wavelength range. The difference of the short circuit current determined from the SR is less than 1% in comparison to a conventional system with monochromator.     

Ultraviolet light emitting diodes and hydrogen peroxide in the photodegradation of aqueous phenol

The novel system of ultraviolet (UV) light emitting diodes (LED) and hydrogen peroxide (H(2)O(2)) was studied for the degradation of phenol as a model organic pollutant in water. The effect of different viewing angles (15 and 120 degrees ), wavelengths (255, 265 and 280 nm) and phenol and H(2)O(2) concentrations were investigated in four photolytic batch reactors. Phenol degradation was observed to be most efficient with UV LEDs emitting at wavelength 280 nm, presumably due to the highest optical power. However, quantum yield for 280 nm reactor was only 0.23 compared to 0.33 of 255 nm reactor. Quantum yields for the rest of the reactors were 0.24 (265 nm, 120 degrees ) and 0.22 (265 nm, 15 degrees ). UV LEDs in combination with hydrogen peroxide are promising in wastewater treatment in degrading organic compounds, though development of both LEDs and reactor design is needed.     

Wavelengths of spectral lines in mercury pencil lamps

The wavelengths of 19 spectral lines in the region 253-579 nm emitted by Hg pencil-type lamps were measured by Fourier-transform spectroscopy. Precise calibration of the spectra was obtained with wavelengths of (198)Hg as external standards. Our recommended values should be useful aswavelength-calibration standards for moderate-resolution spectrometers at an uncertainty level of 0.0001 nm.     

基于误差反馈的LED阵列近场光强检测

发光二极管(LED)在机场、铁路等领域作为信号光源,通常采用多个LED组成阵列的方式。传统方法为拆卸后送入实验室中进行检测,此时无法获得光源在工作现场的状态且使得整个检测周期较长,针对上述问题提出一种基于照度误差的方法。在现场的近场区域内移动照度探头采集照度数据;再对各LED预设光强分布,计算此光强分布下在各测量点形成的照度与利用照度探头实际测量得到的照度的误差并根据此误差来更新设置的光强值,多次迭代至误差足够小;最后将此时计算得到的每个LED的近似光强分布进行叠加用于计算整个LED阵列的光强分布。通过该方法可以在现场快速准确检测灯具光强分布的同时,保障整个检测系统的设计能够便携。通过实验验证,所提方法在不同情况下能够完成光强检测,匹配度达到93%以上。     

Upgradation of a spectral irradiance measurement facility at National Physical Laboratory,India

This report describes the details of a recently upgraded spectral irradiance measurement facility in optical radiation standards at National Physical Laboratory, India. This facility provides the calibration of spectral irradiance in the wavelength range 280 nm – 2500 nm. PTB, Braunschweig, Germany calibrated five numbers of 1000 W quartz halogen lamps, which are used as reference standards for spectral irradiance scale. In addition to providing the details of instruments, the procedure of calibration and evaluation of uncertainties is also described. For checking the fidelity, repeatability and reproducibility of the upgraded system, calibration of one of the PTB calibrated lamps, was done against the other four PTB calibrated lamps. These measurements not only provide confidence on the upgraded system but also verify the retention of the PTB certificate values after a lapse of two years after their calibration.     

Quinine Actinometry as a Method for Calibrating Ultraviolet Radiation Intensity in Light-Stability Testing of Pharmaceuticals

A collaborative study was carried out by seven different laboratories to evaluate quinine actinometry as a universal, standardized method for calibrating UV radiation intensity from light sources used in light-stability testing of pharmaceutical products. Near UV fluorescent lamps, white fluorescent lamps, metal halide lamps and xenon arc lamps were employed as light sources. The increase in absorbance at 400 nm of aqueous quinine solutions was found to be proportional to the integrated UV energy emitted from the light sources. The linearity observed between absorbance and integrated UV energy indictates that quinine actinometry can be used to measure the intensity of UV radiation at wavelengths around 330 nm. The slopes of regression curves of absorbance vs integrated UV energy varied among the lamps used due to differing spectral distributions. Light degradation of nifedipine, a model photosensitive drug, was studied based on quinine actinometry.     

Quinine Actinometry as a Method for Calibrating Ultraviolet Radiation Intensity in Light-Stability Testing of Pharmaceuticals

Abstract

A collaborative study was carried out by seven different laboratories to evaluate quinine actinometry as a universal, standardized method for calibrating UV radiation intensity from light sources used in light-stability testing of pharmaceutical products. Near UV fluorescent lamps, white fluorescent lamps, metal halide lamps and xenon arc lamps were employed as light sources. The increase in absorbance at 400 nm of aqueous quinine solutions was found to be proportional to the integrated UV energy emitted from the light sources. The linearity observed between absorbance and integrated UV energy indictates that quinine actinometry can be used to measure the intensity of UV radiation at wavelengths around 330 nm. The slopes of regression curves of absorbance vs integrated UV energy varied among the lamps used due to differing spectral distributions. Light degradation of nifedipine, a model photosensitive drug, was studied based on quinine actinometry.     

Improvement of brightness with Al2O3 passivation layers on the surface of InGaN/GaN-based light-emitting diode chips

In this study, sputtered 50, 70 and 90 nm thick Al₂O₃ thin films were evaluated as a passivation layer in the process of InGaN-based blue as LEDs (Light-Emitting Diodes) in order to improve the brightness of LED lamps. For packaged LED lamps, lamps with Al₂O₃ passivation layer had higher brightness than ones with SiO₂ passivation layer, and LED lamps with 90-nm Al₂O₃ passivation layer were the brightest among four kinds of lamps. Although lamps with Al₂O₃ passivation layer had a bias voltage 0.25 V at 20 mA forward current higher the lamps built with SiO₂ passivation layer, their brightness was improved about 13.6% higher than the conventional LEDs with no change in emitting wavelength.     

Scattering delay time of Mie scatterers determined from steady-state and time-resolved optical spectroscopy

We have determined the scattering delay time of Mie scatterers (r = 255 nm quartz spheres in polyester resin) from a combination of steady-state (integrating-sphere) and time-resolved (frequency-domain) measurements performed in the multiple-scattering regime. The effective transport velocity of light was derived from intensity and phase measurements at four different wavelengths by using the time-integrated microscopic Beer-Lambert law. We could demonstrate a systematic underestimation of the effective transport velocity compared with the phase velocity in the medium. Assuming that this discrepancy was caused entirely by the transient nature of a single-scattering process, the data presented resulted in time delays of between 18 fs (lambda = 678 nm) and 177 fs (lambda = 1,064 nm) per scattering event. For three out of four wavelengths investigated, the measured values are in excellent agreement with values predicted by a theoretical model for the scattering delay time based on Mie theory.     

Metallic colloid wavelength-ratiometric scattering sensors

Gold and silver colloids display strong colors as a result of electron oscillations induced by incident light, which are referred to as the plasmon absorption. This absorption is dependent on colloid-colloid proximity, which has been the basis of absorption assays using colloids. We now describe a new approach to optical sensing using the light scattering properties of colloids. Colloid aggregation was induced by avidin-biotin interactions, which shifted the plasmon absorption to longer wavelengths. We found the spectral shift results in changes in the scattering at different incident wavelengths. By measuring the ratio of scattered intensities at two incident wavelengths, this measurement was made independent of the total colloid concentration. The high scattering efficiency of the colloids resulted in intensities equivalent to fluorescence when normalized by the optical density of the fluorophore and colloid. This approach can be used in a wide variety of assay formats, including those commonly used with fluorescence detection.     

Irradiance of Horizontal Quartz-Halogen Standard Lamps

Spectral irradiance calibrations often require that irradiance standard lamps be oriented differently than the normal calibration orientation used at the National Institute of Standards and Technology and at other standards laboratories. For example, in solar measurements the instruments are generally upward viewing, requiring horizontal working standards for minimization of irradiance calibration uncertainties. To develop a working standard for use in a solar ultraviolet intercomparison, NIST determined the irradiance of quartz-halogen lamps operating in the horizontal position, rather than in the customary vertical position. An experimental technique was developed which relied upon equivalent lamps with independent mounts for each orientation and a spectroradiometer with an integrating sphere whose entrance port could be rotated 90° to view either lamp position. The results presented here are limited to 1000 W quartz-halogen type lamps at ultraviolet wavelengths from 280 nm to 400 nm. Sources of uncertainty arose from the lamps, the spectroradiometer, and the lamp alignment, and increased the uncertainty in the irradiance of horizontal lamps by less than a factor of two from that of vertical NIST standard lamps. The irradiance of horizontal lamps was less than that of vertical lamps by approximately 6 % at long wavelengths (400 nm) to as much as 12 % at the shortest wavelengths (280 nm). Using the Wien radiation law, this corresponds to color temperature differences of 15.7 K and 21.3 K for lamps with clear and frosted envelopes, respectively.     

Uncertainty evaluation for the spectroradiometric measurement of the averaged light-emitting diode intensity

An uncertainty evaluation is presented for the spectroradiometric measurement of the averaged LED intensity (ALI), which is a standardized photometric quantity of LEDs introduced by the Commission Internationale de l'Eclairage. Using a spectral irradiance standard lamp as a calibration source for the spectroradiometer, 12 uncertainty components are sorted out and their propagation formulated with correlations between the components taken into account. The procedure of uncertainty evaluation is demonstrated for four LED samples of different colors; red, green, blue, and white. The relative uncertainties of the ALI of the test samples are determined to be in a range from 4.1% to 5.5% (k=2), but most of their dominant uncertainty components turn out to be systematic and correlated. In conclusion, correlations between the uncertainty components critically affect the overall uncertainty of the LED measurement using a spectroradiometer.