Development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) on rice milling components and by-products: Effects of diet and temperature

Development of Tribolium castaneum (Herbst), the red flour beetle, was assessed on different rice components and their various by-products (i.e., diets) commonly found in rice mills, in two separate experiments. In the first experiment, eggs did not develop through to the adult stage on rough rice hulls, paddy rice dust, and milled rice dust, while eggs developed to the adult stage to some extent on rice flour, milled whole kernels, brown rice, milled broken kernels, and bran. For the diets where development occurred, the lowest percentage was on brown rice, and adults that emerged on brown rice had smaller elytra compared to adults emerging on the other diets. In the second experiment, 1–2-day old neonates were exposed on the diets listed above and held at 22, 27, 32, and 37 °C. At all temperatures, development to the pupal and adult stages was slowest on rice flour. At 22 °C, development to those stages took about twice as long compared to development at 27 °C. As temperature increased developmental times were reduced. Even though neonates developed slowest on rice flour adult emergence rates were not affected. Predictive models were used to estimate potential population development on the diets. At 22 and 27 °C, adult size as measured by elytra length was greatest when they developed on bran, while body weight was generally lowest for adults that developed on brown rice. Results show that T. castaneum can utilize rice components and by-products produced during the rice milling process, although not all components or by-products were optimal for development, and emphasis should be placed on cleaning and sanitation to remove food sources to limit infestations. Mill managers can use these results to show the importance of sanitation, and potentially improve overall pest management programs inside the mill.     

Impact of heat-moisture treatment applied to brown rice flour on the quality and digestibility characteristics of Korean rice cake

Effect of heat-moisture treatment (HMT) of brown rice flour on the quality and digestibility of Korean rice cake (jeolpyeon) was investigated. Brown rice flour with 20% moisture content was heat-moisture treated at 100–110 °C for 1–2 h, and subsequently cooled to 4 °C for 1–2 h. The lightness of HMT rice cake decreased as HMT temperature and time increased, whereas redness and yellowness increased. The hardness, springiness, and cohesiveness of HMT rice cake were much lower than those of brown rice cake. HMT of rice cake caused an increase in the resistant starch content and a decrease in the rapidly digestible starch content. In sensory evaluation, hardness of HMT rice cake was substantially lower than that of rice cake made with untreated brown rice due to retardation of retrogradation. However, the overall acceptability of HMT rice cake was lower than that of white rice cake or brown rice cake.     

Efficacy of methoprene for multi-year protection of stored wheat,brown rice,rough rice and corn

Hard red winter wheat, brown rice, rough rice, and corn were treated with the insect growth regulator (IGR) methoprene at rates of 1.25 and 2.5 ppm, held for 24 months at ambient conditions in buckets on the floor of a grain bin, and sampled every two months. Bioassays were done by exposing 10 mixed-sex adults of Rhyzopertha dominica (F.), the lesser grain borer, and Tribolium castaneum (Herbst), the red flour beetle, on wheat, R. dominica and Sitotroga cerealella (Oliver), the Angoumois grain moth, on brown rice and rough rice, and T. castaneum and S. cerealella on corn. Sample size for all commodities was about 80 g, and these samples were held for 3 months at 27°C-60% r.h. Both rates of the IGR completely suppressed adult progeny development of R. dominica with little resulting feeding damage, sample weight loss, or insect damaged kernels (IDK). Some adult progeny production of S. cerealella and resulting IDK occurred at both rates on rough rice, brown rice, and corn, but was far less than in untreated controls. There was little adult progeny production but some feeding damage caused by larval T. castaneum in the treated wheat and corn but again far less than in untreated control. Allowing continual exposure of parental adults on grains treated with an IGR, rather than exposing those parental adults for a short time period, may give more accurate evaluations of residual efficacy. Results show that methoprene used as a grain protectant will give residual control of stored product beetles for 24 months, but complete control of S. cerealella may require inclusion of a contact insecticide.     

Microwave radiation for control of Tribolium confusum in wheat and flour

The susceptibility of Tribolium confusum Jacquelin du Val to microwave energy was determined by irradiating vials of infested wheat or flour near the aperture of an optimum gain horn coupled through a waveguide to a magnetron operating at 8·5 GHz and 30 W average output at a pulse repetition frequency of 4000 Hz. Mortality was a function of exposure time and wheat moisture content. Adult mortality was higher in insulated samples of wheat than in non-insulated samples. Susceptivility of stages to radiation at 60°C was: eggs > pupae > adults > larvae. Survival of pupae near the surface of wheat indicated that heat was distributed non-uniformly. Mortality was higher in wheat than in white flour. Adults from 1·5 to 7 months old were equally susceptible to radiation.     

Production of high-fructose rice syrup and high-protein rice flour from broken rice

The objective of this study was to develop a process for the production of both high-fructose rice syrup and high-protein rice flour from broken rice. The rice flour was obtained from broken rice by using either a dry or wet milling method. The glucose produced from the slurry of various raw materials by treating with α-amylase and glucoamylase was compared. Results indicated that cassava and corn starch were better raw materials than rice flour. However, the filtered residue of liquefied rice slurry could be recovered as high-protein rice flour. The particle size of rice flour had a small effect on the glucose yield. The orthogonal-array table (L₂₇) method of experimental design was employed to determine optimum conditions for liquefaction. The glucose yield based on starch was 90.8±3.6% under the following optimum conditions α-amylase, 0.12%; rice flour, 20%; temperature, 96°C; time, 90 min. The filtrate from liquefied rice slurry was saccharified at 60°C with three different concentrations of glucoamylase. The higher the enzyme concentration, the shorter the time required to reach the maximum yield. After saccharification, the glucose solution was decolourised, desalted and concentrated to 40% d.s. and then isomerised to fructose at 60°C under continuous operation by using immobilised glucose isomerase packed in a column. The isomerised syrup was then purified and concentrated to 71% d.s. The final high-fructose rice syrup contained 50% glucose, 42% fructose and 3% maltose. After liquefaction, the rice slurry was centrifuged and the precipitate was dried by either spray or drum drying. The composition of these two high-protein rice flours was almost the same and the protein content was about three times as high as the raw material. There were significant differences in surface structure of rice flour and high-protein rice flours, as observed by the scanning electron microscope.     

Development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) on sorghum milling fractions

A series of tests was conducted to determine if Tribolium castaneum (Herbst), the red flour beetle, could survive on six milled sorghum fractions: Bran, Coarse Grits, Fine Grits, Flour, Red Dogs, and Shorts. In the first test, parental adults were exposed on the fractions, removed, and then the fractions were held for six-seven weeks at 27 °C. Late instar larvae and progeny adults were present in all fractions. In the second test, at least 80% of single neonates (1-2-day-old larvae) held on 1 g of a fraction at 27 °C for seven weeks were able to complete development to the adult stage. In the final test, individual neonates were held on 1 g of a fraction at 37 °C, 32 °C, 27 °C, or 22 °C. Time to adult emergence at each temperature ranged from 17 to 23 days, 21–27 days, 28–50 days, and 67–113 days, respectively, depending on the specific fraction. Logistic functions were compared for mean developmental times for each temperature-fraction combination. The six fractions were also analyzed for ash, fat, fiber, moisture, protein, and starch content. The fractions varied with respect to these chemical constituents; fat and moisture content were negatively correlated with development in some comparisons, though overall there was no correlation between these chemical components and neonate development on the fractions. Temperature had an obvious effect on neonate development, which has implications for assessing risk of pest infestations inside sorghum mills during warmer months of the calendar year. In addition, T. castaneum will reproduce and develop on all of the sorghum fractions included in this study, which are commonly generated during the sorghum milling process. Sanitation and removal of residual materials such as the measured fractions could also help with overall pest management of T. castaneum.     

Studies on the isolation of diapause and non-diapause strains of Trogoderma granarium everts (Coleoptera,Dermestidae)

By continuous mass selection and inbreeding, populations of Trogoderma granarium larvae that showed nearly 100 per cent incidence of diapause or 100 per cent freedom from diapause when reared in isolated or crowded conditions on wheat flour at 30°C, were obtained. The progress of selection showed that diapause is a polygenic character. The density-dependent diapause was manifested even in populations in which the density-independent diapause was totally eliminated by selection. In standard experimental populations most larvae enter diapause at 30°C and pupate promptly at 35°C when reared in isolation on a suboptimal diet viz. wetted-dried wheat flour. In contrast, the selected non-diapause population neither entered diapause at 30°C nor pupated at 35°C when fed on this diet; instead, at both the temperatures most larvae either died in the first stadium or survived for long periods with little gain in weight.     

Influence of temperature and artificially-created physical barriers on the efficacy of synergized pyrethrin aerosol

The dispersal of aerosol insecticides within a flour mill may be hampered by barriers created from machinery and other equipment that block dispersion. Additionally, seasonal temperature variations may influence aerosol dispersion and subsequent effectiveness against stored product insects. The influence of barriers and temperatures on the efficacy of synergized pyrethrin aerosol against adults and pupae of the confused flour beetle, Tribolium confusum (Jacquelin du Val), was evaluated in experimental sheds. Insects were exposed to the aerosol at target temperatures of 22, 27, and 32 °C. Wooden boxes 1 m in length, 20 cm in width, and 5, 10, or 20 cm in height were used for creating different open and concealed areas for exposing insects. Results showed that exposing adults of T. confusum in open areas produced more moribund adults (those knocked down and unable to move except for reflex twitching or unable to respond when touched with a probe) at all temperatures, while survival increased with increasing distance from the front to the back of the boxes. Given the decreased survival, it would appear that more aerosol dispersed under the box height of 20 cm compared to 5 and 10 cm box heights because survival was generally less under the 20 cm box compared to the other two. Less than 2% of the pupae exposed in the open emerged as adults, but as the distance increased from the front to the back of the box adult emergence from these exposed pupae increased. Synergized pyrethrin aerosol can provide good control of T. confusum pupae and adults and may not be affected by seasonal temperature variations, but aerosol dispersion into obstructed or concealed areas may affect control.     

Development and validation of a model for predicting survival of young larvae of Tribolium castaneum exposed to elevated temperatures during heat treatment of grain-processing facilities

Heat treatment involves raising the ambient temperature of grain-processing facilities to 50–60 °C for at least 24 h to manage stored-product insects. The young larvae (first instars) of the red flour beetle, Tribolium castaneum (Herbst), are the most heat tolerant stage at temperatures of 50–60 °C when compared to eggs, old larvae, pupae, and adults. A thermal death kinetic model was developed to predict survival of T. castaneum young larvae exposed to six constant elevated temperatures between 42 and 60 °C. The model was based on two non-linear relationships: 1) logarithmic decrease in survival of larvae as a function of time, and 2) logarithmic decrease in larval survival as a function of temperature. The model was validated with 12 time-dependent temperature datasets collected during actual heat treatments of pilot-scale flour and feed mills and a commercial grain-processing facility. The observed survival of larvae in plastic boxes/vials with flour was used to validate model predictions. The heating rate to reach 50 °C from the ambient temperature, among the 12 datasets ranged from 0.9 to 7.8 °C/h. The mean absolute deviation between observed and predicted larval survival for 10 of the 12 datasets ranged from 2.1 to 11.4%; for the other two datasets it was 16.2 and 18.3%. These results show that the thermal death kinetic model can be used to predict survival of young larvae of T. castaneum based on time-dependent temperature profile obtained at any given location during heat treatment of grain-processing facilities.     

富铬发芽糙米粉性质分析及其在馒头中的应用研究

目的 探究富铬发芽对糙米粉性质的影响及其应用特性研究。方法 通过单因素实验明确发芽糙米中有机铬最佳富集工艺, 对普通糙米粉、普通发芽糙米粉、富铬发芽糙米粉的热力学性质、结晶结构、短程有序结构、水解氨基酸总含量及表面微观结构进行比较。将富铬发芽糙米粉应用于馒头制作, 并对糙米馒头的质构分析、感官评分进行研究。结果 糙米在浸泡时间12 h、发芽温度30℃、三氯化铬溶液质量浓度为80 mg/L时, 有机铬含量达到最高0.266mg/kg, 此时有机铬转化率为50.7%。富铬发芽糙米粉与普通糙米粉相比, 糊化焓值升高14.49%。水解氨基酸总含量升高3.37%。在小麦粉中添加20%比例富铬发芽糙米粉使馒头的硬度升高52.30%, 胶黏性升高30.64%, 咀嚼性升高32.21%, 弹性降低32.30%。此时糙米馒头的感官评分最高, 为87.7分, 最易被人们接受。结论 富铬发芽过程并未改变糙米粉的理化性质, 20%糙米粉添加比例接受度最高, 这可为富铬发芽糙米粉的应用提供参考依据。     

Parboiling improved oxidative stability of milled white rice during one-year storage

Parboiling was investigated to improve the long-term oxidative stability of milled white rice beyond its current application to paddy brown rice. White rice was steamed at 105°C for 20 min, 120°C for 5 min, or 120°C for 20 min without soaking in water, followed by drying at 70°C for 3 h. During oneyear storage at 25 and 4°C, acid value as an index of oxidative deterioration of rice remained relatively constant in parboiled rice samples, while significant increases were observed in raw rice with higher acid values at 25°C compared with 4°C. This indicates that the parboiling improved the oxidative stability of white rice over the long period of time even at room temperature, therefore it can be an efficient alternative to retard the oxidative deterioration of white rice.     

Freezing and Thawing Conditions Affect the Gel Stability of Different Varieties of Rice Flour

The freeze‐thaw stabilities of three different rice flour gels (amylose rice flour with 28% amylose, Jasmine rice flour with 18% amylose and waxy rice flour with 5% amylose) were studied by first freezing at –18 °C for 22 h and subsequent thawing in a water bath at 30 °C, 60 °C and 90 °C, or by boiling in a microwave oven. The freeze‐thaw stability was determined for five cycles. Starch gels thawed at higher temperature exhibited a lower syneresis value (percent of water separation) than those thawed at lower temperature. Amylose rice flour gels gave the highest syneresis values (especially at the first cycle). The Jasmine rice flour gels gave a higher syneresis value than the waxy rice flour gel. Except for freezing by storage at –18 °C and thawing at 30 °C, there was no separation of water at any cycle when waxy rice flour gel was thawed at any temperature, irrespectively of the freezing methods used. Cryogenic Quick Freezing (CQF) followed by storage at –18 °C and then thawing (by boiling or by incubation at any other temperatures) gave lower syneresis values than all comparable samples frozen by storage at –18 °C. The order of syneresis values for the three types of rice flour was waxy rice flour < Jasmine rice flour < amylose rice flour. The syneresis values and the appearance of starch gels, which had gone through the freeze‐ thaw process, suggested that the order of freeze‐thaw stability of gels for the three types of rice flour was waxy > Jasmine > amylose rice flour.     

Age-related susceptibility of Tribolium castaneum (Herbst) to synthetic C18 juvenile hormone

Larvae of ten different age groups and pupae of four were kept in flour containing 30 ppm synthetic C₁₈ juvenile hormone (JH-1) for four days. Newly emerged adults were similarly kept in flour containing 100 ppm JH-1 for two weeks. Newly laid eggs (0–24 hr old) were placed for eight days on filter papers impregnated with 6·4 μg/cm² synthetic JH-1. All insects were kept at 25°C and 70% r.h. Larvae exposed when they were 25 days old or older and pupae exposed during the first seven days of the pupal period suffered high mortality and a large number of deformed adults were produced. All other larval and pupal stages were apparently unaffected by the hormone. Treated eggs hatched normally and newly emerged adults survived the treatments and produced normal numbers of offspring. The potential value of juvenile hormones as control agents for Tribolium castaneum is discussed.     

Paddy rice stored under hermetic conditions: The effect of relative humidity,temperature and storage time in suppressing Sitophilus zeamais and impact on rice quality

The aim of this study was to analyse the effect of relative humidity in suppressing Sitophilus zeamais, in paddy rice stored under hermetic conditions, during four and seven months, at different average temperatures, as well as the impact on rice quality.Hermetic bags, GrainPro^® SuperGrainbag^® Farm™, were used to store two rice varieties under three different relative humidities: 67%, 75% and 85% RH, and average temperatures of 14 °C, 17 °C and 24 °C, both monitored by Hobo^® Data loggers, with the probe placed inside the bags. CheckpointII Portable O₂ and CO₂ Gas Analyzer was used to assess gas contents on the top and bottom of each bag. At the end of the trials, paddy samples were collected to estimate water activity (a_w). The rheology behaviour of rice pastes prepared with race flour obtained from the different treatments was also evaluated, using a controlled stress rheometer.The results showed that the response of the stored-product insects changes with environmental conditions, O₂ and CO₂ contents. Other parameters were considered; a_w increased with relative humidity and temperature, but decreased with storage time. The relative humidity played an important role, together with the increase of temperature, in suppressing insect populations. A modified atmosphere was naturally produced inside the hermetic bag, under 85% RH, with low O₂ and high CO₂ contents, at different average temperatures, 14 °C and 17 °C. These results demonstrated that S. zeamais can survive, but has no progeny. Under the same conditions, but at the higher average temperature of 24 °C, S. zeamais attained 100% mortality before producing progeny.The increase on respiration rate, registered by CO₂ increase and O₂ decrease, for higher RH values, reduced the viscoelastic functions and changed the starch gelatinization point of Indica and Japonica rice.The results obtained showed that storing paddy hermetically, at low relative humidity, did not change atmospheric content and maintained the viscoelastic functions of the rice pastes.     

Cold hardiness of immature and adult stages of the Mediterranean flour moth,Ephestia kuehniella

The cold hardiness profile of immature and adult stages of the Mediterranean flour moth, Ephestia kuehniella was investigated in the laboratory. Supercooling point (SCP) of early instars, late instars, pupae and adults of E. kuehniella was determined using a circulating bath with a cooling rate of 1 °C/min. Mean SCP of pupae was significantly lower (−23.3 °C) than that for early and late instars (−16.1 and −19.5 °C, respectively), but did not differ significantly from that for adults (−21.6 °C). Moreover, mortality at sub-zero temperature was estimated by cooling eggs, early instars, late instars, pupae and adults to −5, −7.5, −10 and −12.5 °C for 30, 60, 90 and 120 min. Main effects of temperature, exposure time and developmental stage on mortality proved to be significant. Two-way interactions as well as the three-way interaction between all tested factors also proved to be significant in most cases. Generally, pupae and adults were the most cold-tolerant, followed in decreasing order by late instars, early instars and eggs. However, when exposure temperature declined to −12.5 °C, no significant differences were observed between the developmental stages in any exposure, suggesting that temperatures as low as −12.5 °C are equally detrimental to all developmental stages. Complete mortality was observed only when early instars, late instars and adults were exposed to −12.5 °C for 120 min. In all tested temperature regimes mean lethal time (LTime₅₀) of pupae was higher compared to the other developmental stages. Similarly, in all exposure times mean lethal temperature (LTemp₅₀) of pupae was lower in relation to the other stages. Non-freezing injury above the SCP was well documented for all stages of E. kuehniella indicating a pre-freeze mortality. The potential of using low temperatures to control E. kuehniella is discussed.     

Feasibility of detection of infested rice using an electronic nose

Electronic nose (E-nose) technology has a potential to be used for detecting insects in stored rice. It may provide a new method to detect the insect activity by providing smell prints for the headspace volatiles in rice mass. The objective of this research was to study the feasibility of using E-nose to detect infested rice during the storage process. An E-nose instrument (Cyranose 320) consisting of 32 sensors for detecting the volatile components produced in infested rice was used for this study. Storage rice with moisture content of 11.8 ± 0.3% was used for conducting the tests. The rice samples were infested with different numbers of adult insects, including rice weevil (Sitophilus oryzae), lesser grain borer (Rhyzopertha dominica) and red flour beetle (Tribolium castaneum), in containers containing 1 kg of rice. The samples were stored at temperatures of 15 °C and 30 °C for four weeks. Air samples in the containers were taken for measurements every five days. The data were analyzed using the principal component analysis (PCA) method. The results revealed that the E-nose was not able to distinguish the clean rice and infested rice with lesser grain borer. While the E-nose had high response ability to the volatile components produced by rice weevil and red flour beetle after four weeks of storage. Consequently, the E-nose could discriminate the clean and infested rice with above insects after four weeks of storage at temperature of 30 °C. Additionally, under the aforementioned conditions, the E-nose could not distinguish the clean and infested rice during the first, second and third weeks of storage. The classification accuracy of E-nose for clean and infested rice was low at storage temperature of 15 °C for the tested insets. It can be concluded that the E-nose has the capability to detect infested rice after four weeks of storage.     

The effects of germination,fermentation, and fermentation additives on the phytate content of rice flour

The phytate contents in nine rice cultivars (Oryza sativa cv.) grown in Korea were investigated and the effects of germination, fermentation, and fermentation additives on the phytate content of rice flour were studied. In nondehulled paddy rice, the phytate content was the highest in Goamibyeo and the lowest in Hanareumbyeo. In milled rice, the phytate content was the highest in Suweon517 and the lowest in Manmibyeo. For Goamibyeo, which had high phytate contents both before and after milling, there was a significant (p<0.05) reduction in the phytate content of paddy rice after germination. The phytate content reduction occurred when germinated paddy flour was fermented at 50°C for 24 h. Paddy rice phytate was completely removed when ammonium sulfate was added to germinated paddy rice flour, which was then allowed to ferment at 50°C for 24 h.     

Antistaling of rice starch in a gel model system and Korean rice cake: the role of wheat flour in retrogradation-retardation technology

This study focused on the mechanisms of retrogradation-retardation technology for applications in garaetteok (steamed and extruded cylinder-shaped rice cake) production. Based on the comparison of conventional and retrogradation-retardation methods, the addition of raw wheat flour was selected as a key process. The rheological properties of gelatinized rice starch pastes after treatment with raw or autoclaved wheat flour and Novamyl L were investigated. Among them, raw wheat flour and Novamyl L resulted in significantly lowered the elastic modulus. The textural properties of garaetteok treated with raw or autoclaved wheat flour were examined by texture profile analysis (TPA) during storage for 3 days at 4°C. The hardness of garaetteok made with raw wheat flour was maintained for up to three days. In contrast, autoclaved wheat flour did not affect elastic modulus or hardness. As a result, raw wheat flour effectively retards starch retrogradation through starch hydrolysis induced by amylase in wheat flour.     

不同温度下充氮低氧对赤拟谷盗各虫态致死效果与时间研究

了解低温对充氮低氧气调致死害虫过程和时间的影响程度,以及氮气气调过程中害虫异常行为反应与种群完全致死时间的关系可为低温充氮(低氧)与成功杀虫应用提供指导。分别在低温18℃、准低温23℃和常温28℃条件下,测定了赤拟谷盗的卵、幼虫、蛹、成虫各个虫态在控制氮气体积分数98%(氧气2%以下)过程中不同时间的死亡率及完全致死时间,以及视频监测下成虫行动异常和击倒反应率与种群完全致死的关系。98%氮气体积分数下,18、23、28℃时赤拟谷盗卵的死亡率-时间回归方程分别为y=3.61+5.47、y=3.92x+11.11和y=5.95x+5.83,完全致死时间分别为28、24、16 d;幼虫的相应回归方程分别为y=4.62x+10.16、y=6.08x+8.33和y=6.66x+21.67,完全致死时间分别为20、16、12 d;蛹的相应回归方程为y=3.69x+3.81、y=4.11+3.89和y=5.87x+13.33,完全致死时间分别为28、24、20 d;成虫的相应回归方程分别为y=6.33x-1.67、y=5.87x+13.33和y=7.29x+15.56,完全致死时间分别为16、16、...     

Life history of Teretriosoma nigrescens Lewis (Coleoptera: Histeridae) and its ability to suppress populations of Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae)

The life history and behaviour of Teretriosoma nigrescens Lewis are typical of a histerid predator, but only 2, instead of the expected 3, larval instars were recorded. At 27°C, 70% r.h. development from oviposition to adult emergence took about 8 weeks. In 24 h at 27°C, 70% r.h., T. nigrescens adults and larvae, respectively, consumed on average up to 1.7 and 3.5 Prostephanus truncatus (Horn) larvae. After 7 weeks, 10 T. nigrescens adults prevented populations of 25, 50, 75 and 100 P. truncatus from increasing, while controls increased at least 10-fold. Grain moisture contents of between 8.5–14% had little effect on predatory efficiency.