EFFECT OF DIETARY VITAMIN E AND SURFACE APPLICATION OF OLEORESIN ROSEMARY ON IRON/ASCORBATE-STIMULATED LIPID OXIDATION IN MUSCLE TISSUE AND MICROSOMES FROM RAINBOW TROUT (Oncorhynchus mykiss)

The effect of dietary α-tocopheryl acetate and surface application of oleoresin rosemary on iron/ascorbate-induced lipid oxidation in rainbow trout ( Oncorhynchus mykiss ) muscle and microsomes was investigated. The 2-thiobarbituric acid-reactive substances (TBARS) values of muscle and microsomes isolated from fish fed the higher concentration of α-tocopherol (500 mg/kg feed) were smaller than those of fish fed the lower concentration (100 mg/kg feed). The protective effect of oleoresin rosemary on lipid oxidation in fish muscle was also observed. Analysis of variance revealed a significant (p < 0.01) interaction between dietary treatments and incubation time. Dietary α-tocopherol supplementation significantly increased the α-tocopherol concentration of muscle microsomal membranes, thereby increasing the oxidative stability of the membrane lipids.     

EFFECT OF THERMAL PROCESSING ON THE PHENOLIC ASSOCIATED HEALTH-RELEVANT FUNCTIONALITY OF SELECTED LEGUME SPROUTS AND SEEDLINGS

The effect of thermal processing on modification of phenolics-linked functionality of the sprout and seedling extracts of select legumes was investigated. Health-relevant functionality for type 2 diabetes-related α-amylase and α-glucosidase inhibition, hypertension-related angiotensin-converting enzyme 1 (ACE 1) inhibition and ulcer-related Helicobacter pylori inhibition was investigated. Thermal processing via autoclaving resulted in tissue browning, leading to higher total phenolic content and free radical scavenging-linked antioxidant activity. Thermal processing also improved the type 2 diabetes-related functionality. Fenugreek had the highest α-amylase and α-glucosidase inhibitory activity, followed by soybean, fava bean and mung bean. The H. pylori inhibitory activity of all extracts improved substantially after thermal processing with direct correlation to phenolic content and related antioxidant activity. The ACE 1 inhibitory activity and levo-dihydroxy phenylalanine content of most extracts declined with thermal processing.

PRACTICAL APPLICATIONS

Thermal processing by autoclaving improved the total phenolic content and antioxidant activity in fava bean, mung bean, fenugreek, and soybean sprouts and seedlings. It improved diabetes-related α-amylase and α-glucosidase inhibitory activities and ulcer-related Helicobacter pylori inhibitory activity. It reduced the cognitive function-related levo-dihydroxy phenylalanine content and hypertension-related angiotensin-converting enzyme 1 inhibitory activity. Therefore, diet designs for chronic disease management will have to consider thermal processing-linked modification of bioactive ingredient profiles.     

An enzymic method for differentiating thawed and fresh fish fillets

The specific activities of the lysosomal enzymes α-glucosidase and β-N-acetylglucosaminidase were determined in press juices and extracts from fresh and frozen/thawed fillets of cod, saithe, red fish and haddock. For each of the two enzymes it was found that the press juice/extract activity ratio increased following the freezing/thawing process (six to nine times for αglucosidase and three to five times for β-N-acetylglucosaminidase).     

Lipid Peroxidation and Chemical Changes in Catfish (lctalurus puncfatus) Muscle Microsomes during Frozen Storage

An NADH-dependent lipid peroxidation system previously identified in channel catfish muscle microsomes functioned at ?10° in isolated microsomes. Below that, activity was inhibited. The amount of peroxidase activity remaining after thawing previously frozen isolated microsomes or intact catfish fillets was inversely related to storage temperature. Microsome yield and lipid content were lower in the fillets stored at - 10° than -40°. Most microsomal fatty acids, especially PUFAs, gradually decreased in the fish fillets stored at -10°. Prolonged storage of catfish fillets would require storage below -20°. Any thawing would result in reactivation of the peroxidase system.     

Effects of 50% Ethanol Extract from Rosemary (Rosmarinus officinalis) on α-Glucosidase Inhibitory Activity and the Elevation of Plasma Glucose Level in Rats, and Its Active Compound

ABSTRACT:  Rosemary ( Rosmarinus officinalis ) extract with 50% ethanol remarkably inhibited rat intestinal α-glucosidase (sucrase) activity when compared with 31 different herbs and spices (aqueous and 50% ethanol aqueous extracts). Rosemary-distilled extract obtained from 50% ethanol extract by evaporation inhibited α-glucosidase activity in the reaction with both maltose and sucrose. Maltose or sucrose was orally administered, with or without rosemary-distilled extract, to mice at a dose of 20 mg/mouse. A postprandial elevation in plasma glucose levels 30 min after administration of maltose or sucrose plus the distilled extract was significantly suppressed compared with glucose levels in mice that did not receive the distilled extract. A 0.01% aqueous solution of rosemary-distilled extract supplied as drinking water to streptozotocin (STZ)-induced diabetic mice significantly suppressed an increase in plasma glucose levels 4 d after injection of STZ. It was also shown that a 0.01% aqueous solution of the distilled extract inhibited α-glucosidase (maltase and sucrase) in the small intestine of STZ-induced diabetic mice. An active compound with IC₅₀ values of 290 μg/mL (maltase inhibitory activity) and 150 μg/mL (sucrase inhibitory activity) was isolated and identified to be 5,7,3',4'-tetrahydroxy flavone (luteolin). These results suggested that rosemary extract might be a beneficial food material in the prevention of diabetes and obesity.     

Antioxidant activity and α-glucosidase inhibitory potential of onion (<Emphasis Type="Italic">Allium cepa</Emphasis> L.) extracts

In the current study, we evaluated total phenolics, phenolic profile, and oxygen radical absorbance capacity (ORAC) of onion (Allium cepa L.). The inhibitory activity of onion extracts against porcine pancreatic α-amylase and rat intestinal α-glucosidase was also investigated. Ethyl alcohol extract of onion skin had the highest α-glucosidase inhibitory activity, ORAC value and total phenolic content, followed by water extract of skin, ethyl alcohol extract of pulp, and water extract of pulp. The α-glucosidase inhibitory activity of the extracts was compared to selected specific phenolics detected in the extracts using high performance liquid chromatography (HPLC). Quercetin, a major phenolic compound in onion extract had high α-glucosidase inhibitiory activity. The α-glucosidase inhibitory activity of the onion extracts correlated to the phenolic content and antioxidant activity of the extracts. These results suggest that onion which has high quercetin content has the potential to contribute as a dietary supplement for controlling hyperglycemia and oxidative stress-linked diabetes complications.     

New extraction method suitable for immunoblotting analysis of fish allergens

Immunoblotting is a simple method to analyze allergens in biological samples. In previous immunoblotting studies on fish allergens, however, collagen, an important allergen next to parvalbumin (major fish allergen), has not been detected in fish muscle extracts probably due to its unique chemical properties. This study was aimed to develop an extraction method suitable for immunoblotting analysis of fish allergens including collagen as well as parvalbumin. When various extracts from the Japanese eel white muscle were analyzed by SDS–PAGE, heating of the muscle homogenate at 80 °C for 20 min was found to be the most effective method to extract collagen as well as parvalbumin. The same extraction method was also effective for the other five species of fish analyzed (rainbow trout, Japanese horse mackerel, crimson sea bream, Pacific mackerel, and Japanese flounder). Furthermore, parvalbumin and/or collagen were successfully identified as allergens in the six species of fish by immunoblotting using the heated extracts prepared by the method described above. It can be concluded that the extraction method (heating of the muscle homogenate at 80 °C for 20 min) developed in this study is useful not only for analyzing fish allergens by immunoblotting but also for preparing antigens for diagnosis of fish allergy by RAST (radioallergosorbent test).     

Dystrophin Cleavage and Sarcolemma Detachment are Early Post Mortem Changes on Bass (Dicentrarchus labrax) White Muscle

Using specific dystrophin antibodies directed against a conserved C-terminal sequence, we demonstrated that dystrophin of fish white muscle was quickly degraded by 50% within 24h and by 100% within 2 days, in parallel with titin cleavage and alpha-actinin release from Z-disks. These changes were accompanied by sarcolemma detachment from the myofibers in costameres (the structures containing dystrophin) and Z-disks weakening. For muscle stored during 2 to 6 mo before thawing, total dystrophin disappearance was observed at 4°C in <8h. Dystrophin may serve as a marker for stored fish to evaluate post mortem changes or detect a thawing-freezing process.     

EFFECT OF DIETARY COMPONENTS AND SURFACE APPLICATION OF OLEORESIN ROSEMARY ON LIPID STABILITY OF RAINBOW TROUT (Oncorhynchus mykiss) MUSCLE DURING REFRIGERATED AND FROZEN STORAGE

The effect of dietary supplementation with α-tocopherol and surface application of oleoresin rosemary on the lipid stability of rainbow trout ( Oncorhynchus mykiss ) muscle during refrigerated storage (4C) and frozen storage (−20C) for 8 days and 6 months, respectively, was investigated. A significant (p < 0.05) reduction in α-tocopherol concentrations in muscle tissue during frozen storage was observed. The 2-thiobarbituric acid-reactive substances (TBARS) values of muscle samples, with or without surface application of oleoresin rosemary, increased during storage for all dietary groups. A significant (P < 0.05) interaction between dietary treatments and the time of storage was observed. Muscle tissue from fish receiving a dietary supplement of 500 mg/kg diet had the lowest TBARS values. The oxidative stability of the lipids was further improved by applying oleoresin rosemary to the surface of the fish fillets.     

The effect of thawing methods on the quality of eels (Anguilla anguilla)

Physical (colour), chemical (pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid values (TBA)) and microbiological (total aerobic mesophylic bacteria, salmonella, coliform, yeast and mould counts) analyses were carried out on thawed European eel (Anguilla anguilla). Different thawing treatments were used (in a refrigerator, in water, in air at ambient temperature and in a microwave oven). The results obtained were compared statistically with those of fresh fish. pH, TBA and a^∗ values of thawed samples usually decreased significantly (P < 0.05) when compared to the fresh control. Salmonella was not detected in any of the samples. Coliform and mould counts of fresh control and thawed samples were <1 CFU/g. Total aerobic mesophylic bacteria count of all thawed fish decreased. However, the yeast count of the refrigerator–thawed samples increased. The lowest total aerobic mesophylic bacteria and yeast counts were determined in water–thawed samples. Water thawing is therefore suitable for frozen eel.     

冷冻鱼糜蛋白在冻藏中的物理化学变化及其影响因素

较详细论述了冷冻鱼糜蛋白在冻藏过程中因变性而发生的一些重要的物理化学变化，包括肌原纤维蛋白的盐溶性，ATP酶活性，活性巯基，总巯基及二硫键的含量，表面疏水性，粘度等的变化，并在此基础上论述了鱼的鲜度，pH值，漂洗条件，冻结及解冻条件等对这些变化的影响，从分子水平分析了鱼糜蛋白的冷冻变性机理。     

Radiofrequency Thawing of Frozen Minced Fish Based on the Dielectric Response Mechanism

To preserve the quality of frozen food products, the main goal of suitable thawing is to cause the least amount of damage with the shortest possible thawing time. A radiofrequency (RF) system has potential for use in achieving these goals for industrial thawing operations. This study investigated the characteristics of RF thawing, and sought to determine the optimal conditions for RF thawing by clarifying the temperature distributions in blocks of frozen minced fish. The dielectric properties (DPs) of frozen minced fish and the penetration depths (d_p) of thawing were measured under differing methods and conditions. The temperatures measured by the probes were plotted in Surfer and verified by COMSOL simulation. A sharp increase in DPs (ε′ and ε″) was observed at a frequency of 27.12 MHz (from −3 °C to 0 °C), and a striking d_p was found from −15 °C to −5 °C, Electromagnetic waves at this frequency could penetrate our samples easily, and the uniformity of heating was improved at both ranges of temperature. For the size of frozen minced fish blocks that are commonly used in the industry (25 × 15 × 5 cm), the most suitable electrode gap for thawing was found to be 16 cm. Unlike in the results obtained with other thawing methods, the cooked gel properties after RF thawing were unaffected, which proved that the RF system is optimal for use in industrial production.     

The effect of storage on ice and various freezing treatments on enzyme leakage in muscle tissue of rainbow trout (Oncorhynchus mykiss)

In order to study the biochemical changes in fish muscle during ice storage and freezing-thawing processes, the activities of certain marker enzymes in the cell interstitial fluid from muscle tissue of rainbow trout (Oncorhynchus mykiss) were measured. The enzymes analysed were: lysosomal α-glucosidase (E.C. 3.2.1.20), β-N-glucosaminidase (E.C. 3.2.1.30) and acid phosphatase (E.C. 3.1.3.2). The activity in centrifuged tissue fluid (CTF) was compared with the activity in total homogenate. When ice storage was varied between 3 and 14 days, it did not affect enzyme leakage into the CTF significantly. However, there was a distinct difference between fresh fish and fish iced even for only 1 day, which gave increased leakage of marker enzymes. When the ice-stored samples were subject to a freezing-thawing cycle they showed a marked increase in enzyme activity in the press juice. When the freezing process was varied so as to achieve different freezing rates, the slowest freezing rate caused the highest enzyme leakage.     

Studies on fish and pork paste gelation by dynamic rheology and circular dichroism

ABSTRACT:  The muscle paste of fish, pork, and their mixtures were prepared to study the gelling characteristics by dynamic rheological measurement. The gelation mechanisms of muscle paste were also investigated by circular dichroism. Gel formation of fish paste occurred in 2 steps of 5 to 35 and 51 to 90 °C respectively, while pork paste mainly in 1 step of 49 to 72 °C. Gel formation was relative to the α-helix unfolding of myosin, which responded the melting temperatures of 40 and 50 °C for fish myosin and 50 and 60 °C for pork myosin, respectively. Alpha-helix unfolding of myosin was beneficial for gel formation. During gel formation, G ' of muscle paste was linearly related to α-helical content of myosin. The interactions of fish and pork proteins at high temperature (>35 °C) could change the gel forming characteristics of muscle paste. Mixed paste exhibited a similar gelation pattern to individual fish paste with 2 visible increases in G '. Addition of pork could suppress the breakdown of fish gel structure at approximately 50 °C. Mixing pork and silver carp in a certain ratio could improve the gel properties of silver carp products.     

Thawing, Refreezing and Frozen Storage Effects on Muscle Functionality and Sensory Attributes of Frozen Cod (Gadus morhua)

Thawing and re-freezing of cod fillet blocks did not necessarily result in greater deterioration (relative to a ‘once frozen’ control) of cooked fish sensory attributes after 9 months frozen storage at –22°C. Thawed and refrozen fish muscle displayed a faster decline in myofibril protein solubility than once frozen controls and had reduced water-holding capacity but analysis of proton spin-spin relaxation times indicated no change in water location. The decline in protein solubility was not caused by complete protein unfolding. Long thawing times (30 hr) before re-freezing and storage resulted in cooked fish with more gray appearance and more stale flavor. Changes in fish muscle functionality did not enable direct inference of sensory attributes.     

Production of Cell Membrane-Bound α- and β-Glucosidase by Lactobacillus acidophilus

Hydrolytic enzymes, viz. α- and β-glucosidase, were produced from indigenous isolate, Lactobacillus acidophilus, isolated from fermented Eleusine coracana. Production of these enzymes was enhanced by optimizing media using one factor at a time followed by response surface methodology. The optimized media resulted in a 2.5- and 2.1-fold increase in α- and β-glucosidase production compared with their production in basal MRS medium. Localization studies indicated 80% of the total activity to be present in the cell membrane-bound fraction. Lack of sufficient release of these enzymes using various physical, chemical, and enzymatic methods confirmed their unique characteristic of being tightly cell membrane bound. Enzyme characterization revealed that both α- and β-glucosidase exhibited optimum catalytic activity at 50 °C and pH 6.0 and 5.0, respectively. K _m and V _max of α-glucosidase were 4.31 mM and 149 μmol min⁻¹ mL⁻¹ for p-nitrophenyl-α-d-glucopyranoside as substrate and 3.8 mM and 120 μmol min⁻¹ mL⁻¹ for β-glucosidase using p-nitrophenyl-β-d-glucopyranoside as the substrate.     

Comparative studies on the effect of the freeze–thawing process on the physicochemical properties and microstructures of black tiger shrimp (Penaeus monodon) and white shrimp (Penaeus vannamei) muscle

The effects of different freeze–thaw cycles (0, 1, 3 and 5) on the physicochemical properties and microstructures of black tiger shrimp (Penaeus monodon) and white shrimp (Penaeus vannamei) muscle were investigated. White shrimp had greater exudate loss and higher α-glucosidase (AG), as well as β-N-acetyl-glucosaminidase (NAG) activities, than did black tiger shrimp, especially when the number of freeze–thaw cycles increased (P < 0.05). The decreases in Ca²⁺-ATPase activity, sulfhydryl group content and protein solubility with concomitant increases in disulfide bond formation and surface hydrophobicity were more pronounced in white shrimp muscle, than in black tiger shrimp muscle, particularly after five cycles of freeze–thawing (P < 0.05). The shear force of both shrimps was decreased after five freeze–thaw cycles (P < 0.05). The microstructure study revealed that the muscle fibers were less attached, with the loss of Z-disks, after subjection to five freeze–thaw cycles. Therefore, the freeze–thawing process caused denaturation of proteins, cell disruption, as well as structural damage of muscle in both shrimps. White shrimp generally underwent physicochemical changes induced by the freeze–thawing process to a greater extent than did black tiger shrimp.     

Anti-oxidant activity of added tea catechins on lipid oxidation of raw minced red meat, poultry and fish muscle

The comparative anti-oxidative effects of added tea catechins (TC) and α-tocopherol to raw minced red meat (beef and pork), poultry (chicken, duck and ostrich) and fish (whiting and mackerel) muscle on susceptibility to lipid oxidation were investigated during 10 days of refrigerated (4 °C) display. Fresh meats, poultry and fish, purchased from a local market, were trimmed to remove bones, skin and surface fat and minced through a 4 mm plate. The minced muscle of each species was treated with either the addition of 300 mg TC kg^–1 minced muscle (TC300) or 300 mg α-tocopherol kg^–1 minced muscle (VE300). Minced muscle without any additives served as control (C). Oxidative stability (TBARS) was measured at 3-day intervals. Total lipids, fatty acid composition, total iron and haem iron from minced muscle for each species were also analysed. The susceptibility of untreated minced muscle to lipid oxidation was in the decreasing order: mackerel > beef > duck > ostrich > pork ≥ chicken > whiting. This may be because of the different content of total fat, iron and fatty acid composition between species. The TC300 significantly ( P  < 0.05) reduced lipid oxidation compared with controls for all seven species as shown by lower TBARS values. The anti-oxidant potential of TC was two to fourfold greater than that of α-tocopherol at the same concentration and this potential was species dependent. The VE300 showed limited capacity in inhibiting lipid oxidation for pork, chicken, duck and whiting. The results obtained show that TCs are powerful natural antioxidants when used in minced muscle food.     

Effect of Temperatures on Fish Alkaline Protease, Protein Interaction and Texture Quality

The effect of the activity of fish alkaline protease and protein denaturation on texture changes in mullet muscle during heating were studied. The proteolytic enzyme and the urea denatured hemoglobin substrate were incubated at different pH values and various temperatures. The enzyme activity was optimal at pH 8 and at 65°C. The extent of protein-protein interactions in mullet actomyosin solutions increased with increasing temperature. The rate of protein-protein interactions increased sharply as temperature increased from 35°C to 45°C at pH 6. When mullet muscle was heated at various temperatures ranging from 55°-85°C, the shear force value increased (toughening) during the initial stage of heating and then decreased (softening). The toughening of fish flesh is probably due to muscle protein interactions, and the later tenderizing of fish flesh may be explained by alkaline protease activity (hydrolysis) masking the protein denaturation effect. When fish flesh was cooked at 100°C, no tenderizing effect was observed. This was probably due to loss of enzyme activity and only protein denaturation had significant effect on texture quality.     

THE EFFECTS OF DIETARY α-TOCOPHEROL AND SURFACE APPLICATION OF OLEORESIN ROSEMARY ON LIPID OXIDATION AND CHOLESTEROL OXIDE FORMATION IN COOKED RAINBOW TROUT (Oncorhynchus mykiss) MUSCLE

The inhibitory effect of dietary α-tocopherol supplementation (100 and 500 mg α-tocopheryl acetate/kg diet) and an oleoresin rosemary dip on lipid and cholesterol oxidation in cooked rainbow trout ( Oncorhynchus mykiss ) during storage at 4C for 48 h was investigated. The 2-thiobarbituric acid-reactive substances (TBARS) values of cooked fish muscle increased during storage for all treatments. Dietary α-tocopherol supplementation partially inhibited lipid oxidation. Surface application of oleoresin rosemary further enhanced this protective effect. Cholesterol oxides were formed in all the samples following cooking and storage. The major cholesterol oxidation products (COPS) were identified as 7 β-hydroxycholesterol, α- and β-epoxides, and 7-ketocholesterol. Formation of COPS was reduced by dietary supplementation with α-tocopherol and surface application of oleoresin rosemary. Statistical analysis revealed a highly significant (p < 0.01) inhibitory effect of oleoresin rosemary on COPS formation.