牛as1乳酪蛋白基因上游调控序列的克隆及序列分析

应用ＰＣＲ技术从国内小牛胸腺基因组ＤＮＡ中克隆了１．０ｋｂ牛ａｓ１酪蛋白基因的上游调控序列，并进行了序列分析，发现有少量的缺失或突变，其ＴＡＴＡ框和ＣＡＡＴ框均未发生变异，表明已成功克隆了其启动子序列，这为乳腺定位表达的研究奠定了基础。     

应用荧光原位杂交技术定位水稻的端粒相关序列和BAC克隆

用ＰＣＲ扩增得到的水稻端粒相关序列（ＴＡＳ）Ｔａｓ３和一个ＢＡＣ克隆（ＢＡＣ）为探针，与间期核和中期细胞染色体进行荧光原位杂交（ＦＩＳＨ），确定了在染色体上的位置。     

一个编码XPA2同源蛋白的人视网膜cDNA的克隆

用同源筛选法从人视网膜ＣＤＮＡ分子库中克隆到一个与ＸＡＰＣＤＮＡ有高度同泊的ＣＤＮＡ序列,长１３８６ｂｐ,其第一１７－１１７１ｎｔ为一个完整的开放阅读框（ＲＯＦ）,编码３８４个氨基酸,其Ｃ端较ＸＡＰ２长５５个氨基酸,３’ＵＴＲ长２１８ｂｐ１３５５－１３６０ｎｔ为加尾信号序列ＡＡＴＡＡＡ,１３７７－１３８６ｎｔ为ＰｏｌｙＡ序列。由该ＣＤＮＡＯＲＦ推导的蛋白质被命名为ＡＩＰＨ,它在ＧｅｎＢａｎｋ的登录     

人类神经元蛋白P17.3全长cDNA的克隆及表达特征分析

从人胎脑ｃＤＮＡ文库分离到一个长５９５ｂｐ的ｃＤＮＡ，它含一个编码１５３个氨基酸的完整开放阅读框，５′ＵＴＲ长１８ｂｐ，３′ＵＴＲ长１１８ｂｐ，在３′ＵＴＲ中，含有非典加尾信号序列“ＡＡＴＴＡＡＡ”和长１５ｂｐ的ｐｏｌｙＡ序列，由阅读框推导编码蛋白质的分子量为１７．３ＫＤ，等电点为４．８９，由于它与小鼠神经元蛋白ＮＰ１５．６呈高度同源，尤其是二者的推导氨基酸序列一致性高达８１．２％，故将这一新ｃＤ     

不同序列结构的芳香共聚酰胺性能研究

对利用４，４＾’－二氨基二苯醚人微言轻第三单体与对苯二甲酰氯、对苯二胺共缩聚得到的不同序列结构的共聚酰胺的性能进行了研究，通过退偏振光法研究了共聚酰胺在浓硫酸中的临界浓度和相图，用ＴＧＡ研究了共聚酰胺的热稳定性，通过降解规律研究了共聚酰胺在浓硫酸中的稳定性，此外，还研究了共聚酰胺的溶解性。结果表明，不同序列结构的共聚酰胺在性能上差异很大，当共聚酰胺分子中ＰＰＴＡ链段序列长度较长时其液晶性能、热稳定     

一个编码XAP2同源蛋白的人视网膜cDNA的克隆

用同源筛选法从人视网膜ｃＤＮＡ分子库中克隆到一个与ＸＡＰ２ｃＤＮＡ有高度同源的ｃＤＮＡ序列,长１３８６ｂｐ,其第１７—１１７１ｎｔ为一个完整的开放阅读框（ＯＲＦ）,编码３８４个氨基酸,其Ｃ端较ＸＡＰ２长５５个氨基酸;３ＵＴＲ长２１８ｂｐ,１３５５—１３６０ｎｔ为加尾信号序列ＡＡＴＡＡＡ,１３７７—１３８６ｎｔ为ＰｏｌｙＡ序列。由该ｃＤＮＡＯＲＦ推导的蛋白质被命名为ＡＩＰＨ,它在ＧｅｎＢａｎｋ的登录号为：ＡＦ０３８４３７。它与ＸＡＰ２的氨基酸一致率为４２％,相似率为６１．７％。计算机分析结果显示：推导的ＡＩＰＨ蛋白与乙肝病毒Ｘ蛋白（ＨＢＸ）有三个较高同源的区域。其中两个对应于ＨＢＸ的反式转录激活功能结构域。ＡＩＰＨｃＤＮＡ与人体１６种组织ｍＲＮＡ的Ｎｏｒｔｈｅｒｎ杂交结果显示：这个来源于视网膜的转录本也可在骨骼肌和心脏中见到,杂交带长度约１．９Ｋｂ;此外在骨骼肌和心脏及其它１４种组织中还可见到另一个长约５．８Ｋｂ的杂交带     

大熊猫朊病毒基因克隆与序列分析

根据已报道的哺乳动物朊病毒基因序列设计引物对,采用PCR方法扩增了大熊猫的朊病毒基因,将其克隆到T-Easy载体,序列测定及分析表明所克隆的大熊猫PRNP基因(GeneBank收录号为AF327449)片段为795bp,编码264个氨基酸的前体蛋白,推测其分子量约28．5ku。与已报道的牛(GeneBank收录号为AF455119)、绵羊(GeneBank收录号为AF367623)的相应序列作比较分析,核苷酸序列同源性分别为99％和83％,其编码的氨基酸同源性均为100％。在所克隆的大熊猫PRNP基因中未发现与朊病毒敏感性连锁的氨基酸多态性位点。     

鸡痘病毒282E4株基因组末端非必需区的序列测定与分析

将鸡痘病毒（ｆｏｗｌｐｏｘ ｖｉｒｕｓ，ＦＰＶ）２８２Ｅ４株７．３ｋｂ的ＢａｍＨＩ片段经亚克隆后，获得ｐＵＦａ，ｐＫＳＦｂ，ｐＵＦｃ和ｐＵＦｄ四个重组策粒。采用ＡＢＩ ３７７ＤＮＡ测序仪荧光标记法对上述质粒进行了序列测定，并应用ＤＮＡ ｓｉｓ Ｖ７．０同ＧｅｎＢａｎｋ中已知的ＦＰＶ序列和痘苗病毒的Ｃｏｐｅｎｈａｇｅｎ株的全序列进行同源性比较。     

AR（P）序列部分和分布的随机加权逼近

对于自回归系数未知的ＡＲ（Ｐ）序列，本文构造了适当的随机加权统计量，以其条件分布逼近ＡＲ（Ｐ）序列的部分和分布，在相当一般的条件下，证明了其逼近精度可达ｏ（１／√ｎ）。其中ｎ为样本容量。     

天山根瘤菌模式菌株全序列测定及其系统发育

对天山根瘤菌群的模式株Ａ－ＩＢＳ１６ＳｒＤＮＡ的全序列进行了测定，以确定它在系统发育中的地位。实验中设计了６个引物，通过聚合酶链反应扩增天山根瘤菌模式菌株Ａ－ＩＢＳ的１６ＳｒＤＮＡ。应用ＤＮＡ定向测序技术，获得了菌株Ａ－ＩＢＳ的１６ＳｒＤＮＡ全序列，将所得序列与根瘤菌已知种的模式菌株１６ＳｒＤＮＡ全序列一起进行聚类分析，得到了系统发育树状图，在树状图中，菌株Ａ－ＩＢＳ所代表的根瘤菌群与华癸根瘤菌群     

Use of Batch Methods of Verification of Measurement Channels at Nuclear Power Plants

Measurement Techniques - The need for the development of additional regulatory documents for the purpose of increasing the economic efficiency in the operation of nuclear power plants and reducing...     

Understanding and Controlling Certification Risks

Certification is a conformity assessmenttool confirming that products (including services),processes,systems,and personnel meetthe relevant regulatory requirements.A certificationbody is a conformity assessment body engaged inimplementing audits on whether products (includingservices),processes,systems,and personnel meetthe relevant regulatory requirements.Like othereconomic activities,certification is subject to risks.GB/T 27021-2007 Conformity assessmen-Requirementsfor bodies providing audit and certi...     

Determination of Hardness of High-Strength Steels by the Brinell Method. Part 1. Improvement of Measurement Accuracy

Strength of Materials - Based on the experimental studies and the corresponding analysis of regulatory documents governing the hardness measurements by the Brinell method, the methodology for...     

高效液相色谱法测定食品接触材料中三聚氰胺单体迁移量的不确定度评定

依据GB 9690-2009、GB/T 23296.1-2009和GB/T 23296.15-2009,选取含量接近法规限值的代表性样品,探索测定食品接触材料中三聚氰胺单体迁移量的数学模型,对检测过程中引入的不确定度进行了分类和量化,系统全面地评定了各个不确定度分量,得到该方法的相对标准不确定度为0.093。     

Method for Determining the Complete Set of Constants of the Polarized Piezoceramics for a Single Ring-Shaped Sample

Measurement Techniques - Using the ANSYS finite element modeling software package, Russian and foreign methods for determining the constants (modules) of piezoceramics presented in regulatory...     

Integrating process safety with molecular modeling-based risk assessment of chemicals within the REACH regulatory framework: benefits and future challenges

Registration, evaluation and authorization of chemicals (REACH) represents a recent regulatory initiative by the European union commission to protect human health and the environment from potentially hazardous chemicals. Under REACH, all stakeholders must submit (thermo)physical, thermochemical, and toxicological data for certain chemicals. The commission's impact assessment studies estimate that the costs of REACH will be approximately 3-5 billion Euros. The present study advocates the systematic incorporation of computational chemistry and computer-assisted chemical risk assessment methods into REACH to reduce regulatory compliance costs. Currently powerful computer-aided ab initio techniques can be used to generate predictions of key properties of broad classes of chemicals, without resorting to costly experimentation and potentially hazardous testing. These data could be integrated into a centralized IT decision and compliance support system, and stored in a retrievable, easily communicable manner should new regulatory and/or production requirements necessitate the introduction of different uses of chemicals under different conditions. For illustration purposes, ab initio calculations are performed on heterocyclic nitrogen-containing compounds which currently serve as high energy density materials in the chemical industry. Since investigations of these compounds are still in their infancy, stability studies are imperative regarding their safe handling and storage, as well as registration under REACH.     

Dosimetric audits of photon beams in radiation therapy centres in Rio de Janeiro, Brazil

Data related to 11 y of high-energy photon radiotherapy beam dosimetry are presented and analysed. Dosimetric evaluations were carried out using water phantoms and thimble ionisation chambers and are part of the radiation protection regulatory licensing process for medicine facilities of Brazilian government. Measurements were done at reference conditions for a standard absorbed dose of 100 cGy [cGy (=1 rad)]. The absolute per cent deviation between the measured and presumed delivered doses should not exceed the tolerance level of +/-3%. The first dosimetry survey from 1996 to 1998 showed a situation that was an object of concern. Deviations of 22 and 18.7% could be measured, although small deviations were also obtained. After 1998, the improvement in dosimetry quality control by the radiotherapy centres became clear, with most of the deviations situated within the +/-3% range. The decrease in the measured deviations presents the effective success of the Institute of Radiation Protection and Dosimetry audit programme for the improvement in the control of radiotherapy photon beams in Rio de Janeiro. Also, it is possible to recommend to Brazilian regulatory organisation a decrease in the tolerance level for dosimetric deviations in order to achieve a more precise dose delivered to patients in radiotherapy centres.     

紫外分光光度法测定水中化学需氧量的不确定度评定

依据HJ/T 399-2007,选取含量接近法规限值的代表性样品,从基准物质溯源,探索测定水中化学需氧量的数学模型,对检测过程中引入的不确定度进行了分类和量化,系统全面地评定了各个不确定度分量,得到该方法的相对标准不确定度为0.033。