共查询到20条相似文献,搜索用时 968 毫秒
1.
黄山野菊花中绿原酸含量的测定 总被引:8,自引:0,他引:8
采用紫外分光光度法对产于皖南黄山山脉的黄山野菊花中的绿原酸含量进行了测定,本法准确、精密度高、重现性好,绿原酸浓度线性范围5~20 mg/L(r=0.999 6);平均回收率为102.0%,RSD为0.83。该法可作为黄山野菊花及其制剂质量控制的—种方法。 相似文献
2.
3.
4.
采用高效液相色谱法对玩具中5种邻苯二甲酸酯类物质进行同时分离和检测,对样品的提取、净化以及检测的色谱条件进行筛选和优化.以shim-pack VP-ODS为分离柱;流动相为V(甲醇):V(水)=90:10,紫外检测波长为230nm.该方法所测得的5种邻苯二甲酸酯类物质在0.5-1000mg/L内具有良好的线性,检出限达0.34-0.96ug/L.加标回收率在93.00%-105.23%.该方法适合各种玩具中邻苯二甲酸酯类物质的分析. 相似文献
5.
6.
7.
8.
杜仲叶绿原酸提取物对胰脂肪酶活性抑制的研究 总被引:1,自引:0,他引:1
通过对紫外分光光度计的运用来建立一种快速高效,低成本的方法来研究杜仲叶中绿原酸提取物对胰脂肪酶活性的影响。该实验中并未选用大型仪器来研究,而是选用常见的紫外分光光度计,实验过程中本法能达到良好的线性关系,其R=0.9991,能够达到一般实验要求。实验结果得出杜仲叶中绿原酸提取物抑制脂肪酶的IC50值为0.00126 mg/m L,介于奥利司他与标准品之间,且高于标准品。 相似文献
9.
10.
11.
建立了反相高效液相色谱法测定金银忍冬叶、花和果实中的绿原酸含量的测定方法。利用超声波辅助提取金银忍冬叶、花和果实中的绿原酸,采用采用反相高效液相色谱法测定金银忍冬叶、花和果实中的绿原酸含量。结果表明:在流动相为甲醇(A)∶1.0%的冰醋酸溶液(B)=27∶73,色谱柱为Alltima C18Column(250 mm×4.6 mm,5μm),流动相流速为1.0 mL/min,检测波长为327 nm,柱温为25℃的实验条件下测定金银忍冬叶、花和果实中的绿原酸含量分别为:(31.64±2.21)mg/g,(17.45±1.05)mg/g,(5.14±1.39)mg/g,且宁夏金银忍冬植株不同部位中绿原酸类化合物的含量分布为:叶花果实。回收率为99.74%,RSD为0.23%。所建立的方法稳定,操作简单,结果可靠。 相似文献
12.
Iness Jabri-Karoui Brahim Marzouk 《Journal of the American Oil Chemists' Society》2014,91(8):1367-1375
This study was designed to examine physicochemical composition, antioxidant activities and heat stability of corn oil enriched with bitter orange peel. Volatile compounds composition of corn oil flavored with Citrus aurantium peel was investigated. Flavored oil total aroma content (2.6 mg/mg oil) was mainly represented by monoterpene hydrocarbons and limonene was the major one (2.49 mg/mg oil). Flavored oil methanolic extract was characterized by total phenol content of 1.22 mg GAE/kg. Chlorogenic, ferulic and p-coumaric acids were the major phenolic components of the flavored oil extract (34.33, 30.24 and 19.39 %, respectively). It was also characterized by a higher chlorophylls and carotenoids contents than the refined one. Antioxidant activities of methanolic extracts of both samples were determined using four assays: DPPH, reducing power, β-carotene bleaching and metal chelating tests. In β-carotene bleaching and DPPH radical scavenging assays, flavored oil methanolic extract showed higher activities than the control. It was characterized by a total antioxidant activity of 4.08 mg GAE/kg and an EC50 value of 3.14 mg/mg oil. Its concentration providing 50 % inhibition (IC50) was 0.53 mg/mg oil in the DPPH test and 4.08 mg/mg oil in the β-carotene bleaching test. However, refined corn extract showed significantly lower antioxidant activities (p < 0.05). Results of the oxidative stability index showed bitter orange peel effectiveness against thermal oxidation based on the increased induction time observed in flavored oil (5.95). 相似文献
13.
目的:建立蒲公英中咖啡酸和绿原酸含量的HPLC测定方法。方法:采用Agilent ZorbaxSB-C1(85μm,150 mm×4.6 mm),流动相为甲醇-磷酸盐缓冲液(23:77,V/V),pH=4.0,流速0.8 mL/min,检测波长为323 nm,柱温为室温。结果:在0.00780~0.04160 mg/mL范围内咖啡酸含量与其峰面积呈良好的线性关系,平均加样回收率为98.92%;在0.00848~0.04784 mg/mL范围内绿原酸含量与其峰面积呈良好的线性关系,平均加样回收率为99.13%。结论:采用HPLC测定蒲公英中咖啡酸和绿原酸的含量简便、准确、选择性好,可用于蒲公英中咖啡酸和绿原酸的含量测定。 相似文献
14.
It has been reported that diabetes and Sj?gren's syndrome patients exhibit variations in the amount of salivation and in the lipid components in saliva and salivary glands. We examined whether lipid compositions, especially phospholipid ones in the salivary glands of rats varied with aging. We analyzed phospholipid and fatty acid compositions in the salivary glands of young (5 to 6 weeks), adult (20 weeks), and old (50 weeks) rats and biochemical components in their blood. The aging (adult and old) rats had higher triacylglycerol, total lipid, total cholesterol and glucose contents in the plasma than the young one. The aging ones also had higher total lipid contents in the major salivary glands (parotid, submandibular and sublingual glands). They had higher wet weights of the major salivary glands and epididymal fat pads than the young ones, but had lower ratios of the major salivary glands to body weight. All of them had high phospholipid contents in the parotid and submandibular glands as compared to sublingual gland, but the aging ones had lower percentage of phospholipid contents of all salivary glands. Phosphatidylcholine and phosphatidylethanolamine were predominant among the phospholipids in the major salivary glands, and little difference was observed in phospholipid composition among the three groups. Palmitic and stearic acids (saturated acids), and linoleic, oleic and arachidonic acids (unsaturated acids) were major components of fatty acids of phospholipids in the major salivary glands. The aging ones had higher linoleic and lower arachidonic acid contents in the glands than the young one. In summary, the aging rats had higher total lipid contents than the young ones and had lower phospholipid contents of the major salivary glands. The n-6 fatty acid contents differed between aging and young ones. The results suggest that phospholipids in the major salivary glands change with the development of rat. 相似文献
15.
Gerhard Jahreis Jan Fritsche Hans Steinhart 《European Journal of Lipid Science and Technology》1996,98(11):356-359
During one year's period bulk milk samples were collected monthly from three different types of farms: 1. conventional farming, indoor feeding with silages throughout the year. 2. conventional farming, grazing during summer season, 3. ecological farming, grazing during summer season. The milk protein, fat and ash contents were significantly lower in ecologically kept dairy cows. The highest percentage of saturated fatty acids was found in the indoor group, whereas the trans fatty acid and linolenic acid contents were significantly higher in the ecological group. Dairy products are major sources of conjugated linoleic acid (anticarcinogens). There were enormous differences between the indoor group (0.34%) and the ecological group (0.80%). During the year, the most impressive variations were registered for the cholesterol-raising palmitic acid. The benefits for the consumer: low content of cholesterol-raising fatty acids, high content of cholesterol-lowering fatty acids and high percentage of anticarcinogenic fatty acids, cannot be realized by one system of farm management. 相似文献
16.
全球氨基酸市场供需与预测 总被引:1,自引:0,他引:1
概述了近年来饲料添加剂用氨基酸类的现状和今后发展。着重介绍了氨基酸中主要产品蛋氨酸、赖氨酸和苏氨酸在美国、欧盟和日本的生产和市场发展动向。由于这类食品添加剂需求强劲,目前,该市场正处在回升之中。 相似文献
17.
为了探究酶对热反应红烧风味香精滋味的影响,采用高效液相色谱法分析猪肉酶解液中游离氨基酸的含量,并结合感官评价和电子舌分析技术对红烧风味香精进行了滋味鉴定,同时结合电子鼻分析香精的气味轮廓。结果表明,制备红烧风味香精的最优酶复配方式为:加酶量0.3%〔m(木瓜蛋白酶)∶m(风味酶)∶m(菠萝蛋白酶)=2∶3∶1〕(以瘦肉质量为基准)。最优酶复配方式对应的酶解液中,苦味氨基酸含量最多,为29.32 g/L,其次为鲜味氨基酸,含量为10.21 g/L。对于不同酶复配比例下制备的热反应香精样品,通过电子鼻和感官评价数据的主成分分析可以进行较好地区分,而通过电子舌分析的区分效果则不理想。 相似文献
18.
19.