Analysis of volatile components in herbal pair herba schizonepetae-ramulus cinnamomi

Analysis of volatile components in herbal pair (HP) herba schizonepetae-ramulus cinnamomi (HS-RC),single herb HS and RC was carried out by gas chromatography-mass spectrometry (GC-MS) data and chemometric resolution method (CRM).The two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound by CRM.In total,47,61 and 51 chemical components in volatile oil of HS,RC,and HP HS-RC were respectively determined qualitatively and quantitatively,accounting for 90.52%,88.37%,and 88.72% total contents of volatile oil of HS,RC,and HP HS-RC,respectively.The number of the volatile components of HP HS-RC is almost the addition of that of two single herbs,but their relative contents are changed.     

Analysis of volatile components in herbal pair herba schizonepetae-ramulus cinnamomi

Analysis of volatile components in herbal pair （HP） herba schizonepetae-ramulus cinnamomi （HS-RC）, single herb HS and RC was carried out by gas chromatography-mass spectrometry （GC-MS） data and chemometric resolution method （CRM）. The two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound by CRM. In total, 47, 61 and 51 chemical components in volatile oil of HS, RC, and HP HS-RC were respectively determined qualitatively and quantitatively, accounting for 90.52%, 88.37%, and 88.72% total contents of volatile oil of HS, RC, and HP HS-RC, respectively. The number of the volatile components of HP HS-RC is almost the addition of that of two single herbs, but their relative contents are changed.     

Determination of constituents of essential oil from Angelica sinensis by gas chromatography-mass spectrometry

Gas chromatography-mass spectrometry （GC-MS） coupled with chemometric resolution upon twodimensional data was employed to analyze the constituents of essential oils of Angelica sinensis. Constituents in essential oils of Angelica sinensis root were identified by GC-MS with the help of subwindow factor analysis （SFA） method resolving two-dimensional original data into mass spectra and chromatograms. 76 of 97 separated constituents in essential oil of Angelica sinensis root were identified and quantified, and they account for about 91.36% of the total content. The results show that ligustilide, butylene phthalide, 2-methoxy-4-vinylphenol, carvacrol, alloocimene, 2,6,6-trimethylbicyclo-[3,1,1] hept-2-ene are the main constituents in essential oil of Angelica sinensis root.     

Chemical component analysis of volatile oil in drug pair Herba Ephedrae-Ramulus Cinnamomi by GC-MS and CRM

Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volume integration.By means of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.In total,97,62,and 78 volatile chemical components in volatile oil of HE,RC,and DP HE-RC,were respectively determined qualitatively and quantitatively,accounting for 90.08%,91.62%,and 89.76% total contents of volatile oil of HE,RC,and DP HE-RC respectively.It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo[4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol and(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.     

Comparison of simultaneous distillation extraction and solid-phase micro-extraction for determination of volatile constituents in tobacco flavor

1INTRODUCTION Manyresearchershavestudiedcigarettesmokeforspecificcompoundssuchaspolynucleararomatichydrocarbons(PAHs)andtobaccoflavoradditives[15].Tobaccoflavoradditivesarethees sentialmaterialsincigaretteproduction,andtheuseofvarioustobaccoflavoradditivesisoneofthecoretechniquesintobaccoindustry.Overthepasttwodecades,therehasbeenanincreasedinterestinandusageoftobaccoflavoradditives.Tobaccoflavorsareaddedtoimprovethephysicochemicalcharacterofthecigarette,modifythebaseflavorofthesmokeande…     

Determination of constituents of essential oil from Angelica sinensis by gas chromatographymass spectrometry

1INTRODUCTION TraditionalChinesemedicines(TCMs)are veryimportantduetotheirextensiveuseinpre ventingandcuringmanydiseasesforhumans.Al thoughonlysomecompoundsmightberesponsible forpharmaceuticaleffects,traditionalChinese medicinesusuallycontaintoomanycomponentsandformaverycomplexsystem,whichmakesthe screeningandanalysisofbioactivecomponentsex tremelydifficult[1]comparedwiththoseofwesternmedicines.Whensuchcomplexsamplesareana lyzed,itiscommonthatdifferentcontentsof multi componentsandgreatd…     

长白山不同区域人参中挥发性、半挥发性成分的研究（英文）

将气流吹扫微注射器萃取(GP-MSE)与气相色谱质谱(GC-MS)联用,建立了一种快速分析长白山人参中挥发性、半挥发性成分的方法.在长白县人参中,共确定了45种化合物(占总组分含量的87%),其中主要化合物有呋喃类(30%)、酸类(30%)和醛酮类(12%).用该方法测定长白山不同区域人参中挥发性、半挥发性成分显示,不同区域人参中挥发性、半挥发性成分的组成相似,但含量有显著差异.研究表明,GP-MSE样品前处理方法对研究植物中挥发性、半挥发性成分有良好的应用前景,有助于植物资源的开发利用.     

Comparative analysis of chemical components between barks and leaves of Eucommia ulmoides Oliver

The chemical components of the essential oils in the barks and leaves of Eucommia ulmoides Oliver were analyzed and compared by chromatograms and mass spectra technique, heuristic evolving latent projections (HELP), alternative moving window factor analysis (AMWFA) algorithms and normalization method based on the peak areas; the flavones in the barks and leaves of Eucommia ulmoides Oliver were separated on an ODS column by gradient elution carried out with the flow phase consisting of water, methanol and phosphoric acid (0.1%), and their contents were quantitatively determined by standard curve method and diode array detection (DAD) at 362 nm. The results show that 68 and 73 compounds respectively from essential oils of the barks and leaves of Eucommia ulmoides Oliver are identified, and there are 33 mutual compounds among 108 compounds determined. The total contents of these volatile components of the two samples possess 92.9% and 97.75% of the gross of the relevant essential oils, respectively; the contents of the rutin, quercetin and kaempferol in the barks and leaves of Eucommia ulmoides Oliver are 0.016 9, 0.003 6, 0.002 1 and 0.064 4, 0.030 2, 0.010 0 mg/g, respectively, and the determination recoveries are 95.2%–106.2%. The comparative analysis shows that for the barks and leaves of Eucommia ulmoides Oliver, there are significant differences in their components of the relevant essential oils and flavones. Foundation item: Project(20235020) supported by the National Natural Science Foundation of China     

气流式微注射器萃取技术与GC-MS联用直接分析不同植物叶片中痕量的多环芳烃

为实现植物样品中痕量多环芳烃（PAHs）的简单、快速、准确、高效分析,利用气流式微注射器萃取技术（GP-MSE）结合GC-MS检测植物（落叶松、旱柳和侧柏）叶片中的PAHs,通过加标回收率评价了方法的准确性,并通过定量离子色谱图分析了目标物的定量干扰.结果显示：供试6种PAHs的方法回收率分别为60.81%-119.83%,69.26%-116.68%和62.82%-104.71%;相对标准偏差（RSD）分别为0.92%-16.56%,6.71%-12.34%,3.57%-14.06%.定量离子色谱图的分析表明,该方法不需采用其他净化技术,6种PAHs分离效果好,定量离子基质干扰少,可用于植物叶片中PAHs的检测分析.     

ME-101多功能微萃取仪的研究与开发

基于气体吹扫微注射器萃取技术（GP-MSE）、高温加热和半导体冷凝技术,研究和开发了集萃取、净化、浓缩、预分离为一体的气相色谱样品前处理仪器——ME-101多功能微萃取仪.通过对比水蒸气蒸馏法和多功能微萃取仪萃取得到的关苍术根茎中挥发油的气相色谱-质谱总离子流图发现,利用该仪器萃取到的挥发性成分的个数明显多于水蒸气蒸馏法,并且该仪器萃取的主要成分含量高于水蒸气蒸馏法.实验表明,该仪器具有试剂用量少、萃取时间短、富集率高、结构简单、使用方便、使用费用低等特点,适用于医药、农业、食品、质量控制等领域的固体、液体样品的气相色谱样品前处理.     

早开堇菜的挥发性成分分析

用气相色谱—质谱法分析鉴定了早开堇菜的挥发性成分,确定了早开堇菜挥发油中44种化学成分,占挥发油总量的42.33%,采用峰面积归一化法求出了各种成分的相对含量。     

艾叶挥发油提取实验及分析

采用异丙醇抽提结合水蒸气蒸馏技术提取艾叶中的挥发性组分,经气相色谱-质谱联机分析,结果表明:采用该提取工艺获得了丁酸、邻-二甲苯、正丁醚、反式-1-丁氧基-1-丁烯、丁酸-1-甲丙酯等新的挥发油组分。实验表明,该研究提出的方法能很好地提取艾叶中挥发性组分。     

松针中有机氯农药的气流式微注射器直接萃取

以落叶松为研究对象,以10种有机氯农药（OCPs）为目标物,通过对气流式微注射器萃取技术（GP-MSE）和传统方法（索氏提取和硅藻土柱层析净化）进行比较,利用气相色谱质谱技术进行分离检测,评价了GP-MSE用于直接萃取松针样品中OCPs的准确性,并通过定量离子色谱图初步探讨了目标物的分离效果和基质影响.结果表明：GP-MSE方法分析松针中10种OCPs的加标回收率为70.27%-92.47%（4次平均值）,相对标准偏差为3.21%-22.67%（4次平均值）,与传统方法相比,有相当的回收率和重复性;定量离子色谱图分析表明,经GP-MSE萃取的松针加标样品中各OCPs的分离效果好,基质干扰少.     

Chemical component and antimicrobial activity of volatile oil of Calycopteris floribunda

The volatile oil of leaves and barks of Calycopteris floribunda was examined by gas chromatography-mass spectrometry (GC-MS). 52 volatile chemical components in leaves were identified. The antimicrobial assay of oils in the leaves and barks was carried out by disk diffusion method in vitro. The major components (mass fraction) in leaves are caryophyllene oxide (13.79%), n-hexadecanoic acid (11.91%) and β-caryophyllene (10.45%). Ten constituents are identified accounting for about 99.98% of the total volatile oil in the bark. Among these components, n-hexadecanoic acid (59.18%), linolic acid (12.70%) and butyl octyl phthalate (8.21%) are the major constituents. The oils exhibit strong antimicrobial activity and display more potent against bacteria than fungi.     

Comparative analysis of volatile constituents between herbal pair flos lonicerae-caulis lonicerae and its single herbs

Gas chromatography-mass spectrometry (GC-MS) and the chemometric resolution method (alternative moving window factor analysis, AMWFA) were used for comparative analysis of volatile constituents in herbal pair (HP) flos lonicerae-caulis lonicerae (FL-CL) and its single herbs. The temperature-programmed retention index (PTRI) was also employed for the identification of compounds. In total, 44, 39, and 50 volatile chemical components in volatile oil of FL, CL and HP FL-CL were separately determined qualitatively and quantitatively, accounting for 87.22%, 94.54% and 90.08% total contents of volatile oil of FL, CL and HP FL-CL, respectively. The results show that there are 32 common volatile constituents between HP FL-CL and single herb FL, 33 common volatile constituents between HP FL-CL and single herb CL, and 10 new constituents in the volatile oil of HP FL-CL.     

Comparative analysis of volatile constituents between herbal pair flos lonicerae-caulis lonicerae and its single herbs

Gas chromatography-mass spectrometry(GC-MS) and the chemometric resolution method(alternative moving window factor analysis,AMWFA) were used for comparative analysis of volatile constituents in herbal pair(HP) flos lonicerae-caulis lonicerae(FL-CL) and its single herbs.The temperature-programmed retention index(PTRI) was also employed for the identification of compounds.In total,44,39,and 50 volatile chemical components in volatile oil of FL,CL and HP FL-CL were separately determined qualitatively and quantitatively,accounting for 87.22%,94.54% and 90.08% total contents of volatile oil of FL,CL and HP FL-CL,respectively.The results show that there are 32 common volatile constituents between HP FL-CL and single herb FL,33 common volatile constituents between HP FL-CL and single herb CL,and 10 new constituents in the volatile oil of HP FL-CL.     

Chromatographic fingerprint analysis of Fructus Aurantii Immaturus by HPLC-DAD and chemometric methods

An efficient method for quality control of Fructus Aurantii Immaturus (FAI), a famous traditional Chinese medicine (TCM) was established. A simple and reliable high-performance liquid chromatography-photodiode array detector (HPLC-DAD) procedure coupled with chemometric methods was developed for fingerprint analysis, qualitative analysis and quantitative determination of this herb. In qualitative and quantitative analyses, heuristic evolving latent projection (HELP) method was employed to resolve the overlapping peaks of the tested samples. Two bioactive components, namely hesperidin and naringin, are confirmed and determined, together with four flavonoids compounds tentatively identified including two new ones. From fingerprint analysis, the fingerprint data were processed with correlation coefficients for quantitative expression of their similarity and dissimilarity. The developed method based on an integration of chromatographic fingerprint and quantitative analysis is scientific, and the obtained results can be applied to the quality control of herb medicine.     

不同地区烟叶中挥发性成分的分析

采用气流吹扫微注射器萃取技术联用GC-MS对不同地区烟叶中挥发性成分进行分析,并比较其含量.结果显示：烤烟和白肋烟中分别鉴定出67种和62种挥发性成分,其中以醇类和杂环化合物为主要成分.在不同地区相同品种、不同品种、同一品种不同部位烟叶的挥发性成分含量都具有显著的差别.所得结果能为研究烟草成分提供有利的参考依据.     

太赫兹技术在烟叶香型分类中的应用

针对浓香型、中间型和清香型3种香型特征的烟叶,测定不同质量分数烟叶（烟叶与聚乙烯的混合物）的太赫兹透射光谱,并计算得到相应的太赫兹波段吸收系数和折射率谱线.结果发现,不同香型的烟叶对太赫兹时域信号会产生不同的延迟和衰减,所计算得到的吸收系数曲线和折射率曲线的变化规律和香气特征存在一一对应的关系.其中,烟叶香气浓郁程度与样本吸收系数呈正相关性,而与样本折射率呈现负相关性.本研究证实了其在烟叶质量评价方面应用的可能性.     

化学模式识别——岭回归分光光度测定混合酚

将苯酚、间甲酚、对氯酚、邻氯酚和间氯酚等5种挥发酚与MBTH反应显色后,用分光光度法测定,能获极难分辨的混合物的吸收光谱.本文采用聚类分析对波长-吸光度数据进行特征删选后,再以因子分析-岭回归法解析光谱,成功的确定了混合体系中的物种数、种类和含量.将该法用于印染废水中5种酚的同时定性与定量测定,结果令人满意.