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Simultaneous determination of adenine, uridine and adenosine in cordyceps sinensis and its substitutes by LC/ESI-MS 总被引:1,自引:0,他引:1
A simple, sensitive and reproducible high performance liquid chromatography-mass spectrometry coupled with electrospray ionization method for simultaneous separation and determination of adenine, adenosine and uridine was developed. The analytical column is a 2.0 mm× 150 mm Shimadzu VP-ODS column and volume fraction of the mobile phase is 86.5 %water, 12.0%methanol and 1.5%formic acid. 2-chloroadenosine was used as internal standard. Selective ion monitoring mode and selective ion monitoring ions at ratio of mass to electric charge of 136 for adenine, 268 for adenosine and 267 for uridine were chosen for quantitative analysis of the three active components. The results show that the regression equations and linear range are Y=0. 062X 0. 005 and 2.0 - 140.0μg · mL 1for adenine, Y=0. 049X 0. 004 and 4. 0 - 115.0 μg · mL-1 for uridine, Y=0. 154X 0. 014 and 1.0 - 125.0 μg · mL 1 for adenosine. The limits of detection are 0.6 μg · mL 1 for adenine, 1.0μg · mL-1 for uri dine and 0.2 μg · mL 1 for adenosine.The recoveries of the three constituents are from 96.6% to 103.2%. 相似文献
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1INTRODUCTION TraditionalChinesemedicines(TCMs)are veryimportantduetotheirextensiveuseinpre ventingandcuringmanydiseasesforhumans.Al thoughonlysomecompoundsmightberesponsible forpharmaceuticaleffects,traditionalChinese medicinesusuallycontaintoomanycomponentsandformaverycomplexsystem,whichmakesthe screeningandanalysisofbioactivecomponentsex tremelydifficult[1]comparedwiththoseofwesternmedicines.Whensuchcomplexsamplesareana lyzed,itiscommonthatdifferentcontentsof multi componentsandgreatd… 相似文献
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Concentration of heavy metals in blood and urine of rabbit after inhaling three different kinds of cigarette was studied through
the animal passive smoking pattern. The samples were prepared by nitric acid solution digestion and determination of seven
kinds of heavy metals including Hg, Se, Sn, Pb, Cd, Ni and Cr was performed by inductively coupled plasma-atomic emission
spectrometry (ICP-AES). The ICP-AES method was established with good precision and accuracy, relative standard deviation (n=6) was between 2.9% and 5.9%, and the recovery was in the range of 95.0%–104.2%. Concentration of six heavy metals increases
in some extent in blood and urine after period of smoking and the increasing of heavy metals in blood and urine all shows
time dependence. Significantly higher heavy metal levels are observed in the blood and urine of the cigarette inhaling rabbits
in the exposed group. The concentration of six kinds of heavy metals in the blood of the rabbit increases after 16 weeks exposing
to cigarette smoking. Three times of Hg, ten times of Se and trace amount of Pb, Cd, Ni and Cr are detected in the blood after
16 weeks of smoking. For urine samples, about three times of Hg, two times of Se, five times of Pb and trace amount of Cd
are detected after 16 weeks of inhalation of cigarette. Comparatively, higher concentration of heavy metals are detected after
inhaling of Nise cigarette. 相似文献
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