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1.
为优化超期储存小麦和水稻混合发酵生产燃料乙醇的辅料,以发利耐高温酒用干酵母为发酵菌株,采用同步糖化发酵法,研究了小麦添加比例、淀粉酶种类、降黏酶种类及添加方式对于混合发酵的影响。通过测定液化和发酵醪液的相关指标,优化出适合小麦混合发酵的辅料。结果显示,当干物质含量为30%,超期储存小麦添加比例30%,液化阶段使用超强复配淀粉酶(0.26 kg/t粮),同步糖化发酵阶段使用糖化酶(0.68 kg/t粮)、酸性蛋白酶(0.035 kg/t粮),在32 ℃下发酵72 h酒精度可达12.37%vol;在拌料阶段添加蔚蓝木聚糖酶(0.3 kg/t粮),可使蒸馏残液滤速由4.8 mL/min提高至7.8 mL/min,清液干物由8.2%降低至5.7%,可有效减轻后期污水处理压力。  相似文献   

2.
研究以31.0%干物液化醪为原料,开展对糖化酶加量、尿素、酸性蛋白酶、pH值对发酵酒精度的影响。结果显示,糖化酶1.2 kg/t醪、尿素4.0 kg/t醪、酸性蛋白酶0.30 kg/t醪、pH4.5,发酵效果最优,酒精度可达15.82%vol。  相似文献   

3.
为了研究红茶菌在谷物原料发酵制醋中的应用,以玉米粉、豆粕、米糠粕为原料,总酸、不挥发酸及氨基酸态氮含量为评价指标,考察不同原料比例、不同酶制剂添加量对谷物醋发酵的影响。结果表明,采用玉米粉∶豆粕∶米糠粕=50∶45∶5质量比发酵14 d,糖化酶对成品总酸含量有显著性影响(P<0.05),中性蛋白酶对成品中氨基酸态氮含量有显著性影响(P<0.05)。各种酶制剂的添加量为:α-淀粉酶0.4%、糖化酶0.3%、中性蛋白酶1%、酸性蛋白酶0.3%(第一次添加量),在此最佳酶添加量条件下,接种5%红茶菌发酵,制备谷物醋中总酸、氨基酸态氮含量和不挥发酸含量分别为47.90 g/L、6.62 g/L和0.72 g/L。结果说明红茶菌在谷物原料发酵制醋中的应用是可行的。  相似文献   

4.
该实验对小麦和玉米两种不同原料发酵制备乙醇的生产工艺进行了对比,分析了小麦和玉米两种不同原料的组分及关键工艺指标的差异,并对小麦发酵制备乙醇的生产工艺进行了优化。结果表明,在相同的工艺条件下与玉米乙醇相比,小麦乙醇的液化黏度高、发酵酒分低、清液悬浮物高、废醪液量大。最佳小麦发酵制备乙醇的生产工艺为拌浆干物30%,粉碎粒度2.5 mm,木聚糖酶添加量0.05 kg/t小麦,在此优化条件下,小麦发酵制备乙醇的液化黏度约458 cP,发酵成熟醪乙醇含量为11.48 g/100 mL,淀粉出酒率达到51.3%,糖浆黏度为1 249 cP。  相似文献   

5.
通过中维信酸性蛋白酶和隆科特酸性蛋白酶在水稻发酵中试验,检测隆大酸性蛋白酶(添加量为0.06kg/t粉)的发酵效果与添加0.15kg/t粉的中维信酸性蛋白酶发酵效果,经过3批重复试验,综合常规检测结果分析:添加0.15kg/t粉的中维信酸性蛋白酶试验样品发酵效果略优于与添加隆大酸性蛋白酶试验样品的发酵效果,可以应用在水稻燃料乙醇生产中,便于公司采购选择性,减低辅料成本。  相似文献   

6.
为了评估陈化纯淀粉掺入陈化粮生产燃料乙醇的应用效果,通过摇瓶试验,以陈化水稻、陈小麦和淀粉为原料,研究酸性蛋白酶和接种量对于高浓度乙醇发酵的影响。结果显示,以陈化水稻和陈化小麦为基础培养基,添加一定比例的淀粉有利于酒精度的提高,当添加量为40%~50%时,酒精度达到最高(13.4%vol~13.6%vol),总糖消耗较为彻底;采用高浓度淀粉醪液(总糖270 g/L左右)和酸性蛋白酶发酵可以有效提高发酵强度,尤其将接种量提升至40%左右时,最高酒精度可达15.1%vol,出酒率达52%以上。采用50 L发酵工艺验证,发现发酵指标与摇瓶一致,DDGS产品蛋白指标较佳。此研究结果表明将高浓度的淀粉以一定比例掺入到混合陈化粮醪液发酵是可行的,具有较强的工业化应用参考价值。  相似文献   

7.
选取大米生料发酵过程中的主要因素,以发酵结束后的乙醇含量为评价指标,通过单因素试验和正交试验,对大米生料发酵产乙醇的工艺条件进行了研究。结果表明,大米生料发酵产乙醇的最佳工艺条件为原料颗粒度50目,料水比1∶3.0(g∶mL),α-淀粉酶添加量10 U/g,糖化酶添加量200 U/g,酸性蛋白酶添加量30 U/g,酵母液接种量1%,硫酸铵添加量0.8%。在此优化工艺条件下,乙醇含量为10.89%。  相似文献   

8.
大米发酵生产酒精的工艺研究   总被引:8,自引:1,他引:8  
袁敬伟  李春玲  刘辉 《酿酒》2003,30(2):30-31
在大米发酵生产酒精工艺中,淀粉浓度以18%(W/W)为宜,液化酶最佳用量为40μg/g原料,糖化酶最佳用量为120μg/g原料,可适量添加酸性蛋白酶,对提高酒分有促进作用。  相似文献   

9.
以花生粕、小麦麸皮为原料制备花生酱油成曲,研究花生粕与小麦麸皮质量比、米曲霉(Aspergillus oryzae)As3.042接种量、制曲温度和制曲时间4个因素对花生粕酱油成曲中性蛋白酶酶活的影响,并通过单因素试验和响应面试验优化制曲工艺。结果表明,对中性蛋白酶活影响程度从大到小依次为制曲温度>接种量>原料质量比>制曲时间。花生酱油成曲的最佳制曲工艺条件为:花生粕与小麦麸皮质量比8∶2,米曲霉接种量0.65%,制曲温度32 ℃、制曲时间24 h。在此优化条件下,成曲中性蛋白酶活为(2 493.67±7.70) U/g,酸性蛋白酶活为(596.84±23.12) U/g、糖化酶活为(29.91±2.63) U/g、淀粉酶活为(882.85±32.63) U/g、纤维素酶活为(11.72±3.69) U/g。  相似文献   

10.
以萌发麦芽为原料,采用半固态发酵技术酿造纯麦酒,并进行工艺优化及理化性质测定。设计单因素实验研究发酵过程中发酵时间、酒曲接种量以及糖化酶添加量对纯麦酒酒精含量的影响,并确定最佳单因素实验条件。在单因素实验结果的基础上,进行正交试验的设计,确定最佳工艺组合为发酵时间为4 d、酒曲接种量为2‰、糖化酶添加量为2%。在此条件下,所得纯麦酒酒精度为10.6%vol±0.3%vol,总酚含量为1.159±0.066 g/L,蛋白质含量为209.568±19.178 mg/L,总酸含量为6.4±0.2 g/L,还原糖含量为8.409±0.021 g/L,对羟基自由基的清除率可达86.75%±1.42%,总还原能力可达1.842±0.004。正交试验方差分析结果表明发酵时间与糖化酶添加量两个因素对于纯麦酒的酒精度影响显著(P<0.05),而接种量对纯麦酒酒精度的影响并不显著(P>0.05)。试验结果为纯麦酒的生产开发提供理论依据。  相似文献   

11.
Since the discovery of gluten by Beccari in the 18th century, wheat producers, millers, and bakers have tried to find relatively simple methods of prediction of wheat quality, particularly during the breeding stages. Bearing in mind that endosperm proteins play the governing role in determination of wheat quality, this prediction is generally based on these proteins. Early research concentrated on relations among the macrofractions (i.e., gliadin:glutenin ratio, ratio of acetic acid soluble proteins, etc.). Later, the gliadin polypeptides, the genes coding for these polypeptides, and variety identification became the focus of research.

More recent predictions are based on the high-molecular-weight glutenin subunits. Although such predictions are successfully applied in many countries for wheat breeding, the low correlation between predicted and practical quality observed in many cases, and the variability of quality of different wheat varieties, suggests that further studies are needed to improve the reliability of this particular prediction.

In the framework of such studies, more attention should be paid to the synthesis and interactions of gluten-forming polypeptides—particularly with respect to the polymerization process of glutenin subunits; the role of redox-system of kernel in the gluten formation; and the formation of gluten complex, including role of nonprotein constituents; consequently, the wheat kernel should be treated as a biological unit. From the methodological point of view, the uses of new techniques such as immunochemistry, nondestructive evaluation methods, etc., hold promise for improved quality prediction.  相似文献   

12.
Metabolism of 22 free amino acids (AA) during fermentation(early and later steps) of wheat dough samples started with pure and associated cultures of yeast and lactic acid bacteria, and commercial compressed yeast (CY) were investigated. Unfermented and fermented straight doughs were studied byReversed-Phase HPLC of their dansyl derivatives. Used as starters were strains of Saccharomyces cervisiae, Lactobacillus brevis, Lactobacillus plantarum and Enterococcus faecium. Statistical data analysis indicated clustering of samples with yeast and samples without yeast (including uninoculated doughs) respectively, based on rate of metabolism of acidiC., basiC., aliphatic and aromatic A A. Differences in AA metabolism during the later fermentation step (DF2) categorized bacterial starters into groups according to balance between degree of assimilation(D F2<0) and exoproteolytic release(DF2>0) of amino acids.  相似文献   

13.
王小平  雷激  刘刚  孙曼兮  叶丹  陈丽娟 《食品科学》2017,38(15):147-152
为改善麸皮面包的品质,增加麸皮的利用率,本实验应用甜酒曲联合酵母发酵麸皮、黄豆和花生的混合物,得到发酵麸皮混合物。以面粉+发酵麸皮混合物总量为100 g(面粉90 g、发酵麸皮混合物10 g)计,制备发酵麸皮混合物面包(简称发酵麸皮面包),以普通面包、麸皮面包及添加黄豆、花生混合物的非麸皮添加物面包为对照,对这几种面包的品质、风味物质及抗氧化活性进行分析。结果表明:黄豆、花生的添加能够起到提升面包风味的作用;发酵麸皮混合物的添加可以改善麸皮面包的品质,其感官评分、弹性、硬度和色差L、b值极显著优于麸皮面包(P0.01),而与普通面包比较没有显著差异(P0.05);与3种对照面包样品比较,发酵麸皮面包的醇类、酯类和醛类等风味成分种类较多,且分布均匀,而且还检测出其特有的风味物质,分别为吡嗪类、呋喃类、乙基酯类;发酵麸皮面包的多酚、黄酮含量以及抗氧化活性指标也均最高。综上,发酵麸皮混合物能够改善麸皮面包的品质,并提高面包的抗氧化活性。  相似文献   

14.
The cover image is based on the Research Article The Israeli Palestinian wheat landraces collection: restoration and characterization of lost genetic diversity by Sivan Frankin et al., https://doi.org/10.1002/jsfa.9822 .

  相似文献   


15.
G4淀粉酶抑制小麦淀粉老化研究   总被引:1,自引:1,他引:0  
测定G4淀粉酶处理小麦淀粉相关理化性质,如凝沉性、冻融稳定性、糊化特性、质构性、溶胀度和可溶指数等,研究G4酶对小麦淀粉抗老化性。实验结果表明:经G4淀粉酶处理后,小麦淀粉凝沉体积提高57.5%,析水率和粘度均有所降低;随温度升高,两种小麦淀粉溶胀度和可溶指数均呈现上升趋势;但G4酶处理小麦淀粉溶胀度和可溶指数明显高于原小麦淀粉,小麦淀粉凝胶硬度显著降低,G4淀粉酶具有抑制小麦淀粉老化作用。  相似文献   

16.
研究超声波-酸解改性的麦麸粉不同添加量对面粉粉质特性、面团拉伸特性、面粉糊化特性以及面团质构特性的影响。结果表明:随着酸改性麦麸粉添加量的增加,面团的吸水率、形成时间呈上升趋势,稳定时间和粉质指数则先上升后下降;面团的最大拉伸阻力、拉伸阻力、拉力比数随酸改性麦麸粉添加量的增加呈上升趋势,而延伸性和拉伸面积呈下降趋势,对面团的拉伸特性具有正、反两方面的作用;峰值黏度、最低黏度、衰减值、最终黏度、回生值、糊化温度及峰值时间均呈先上升后下降的趋势;质构特性结果表明,面团的硬度、弹性、黏附性、咀嚼性、内聚性和回复性随着添加量的增加呈先增加后减少趋势。综合得出,酸改性麦麸粉添加范围6%~9%可以改善面粉粉质及质构特性。  相似文献   

17.
通过对黄淮麦区2012年收获的313份高产强筋小麦样品进行全面分析和评价,结果表明,小麦样品的千粒重平均值为(39.38±4.12)g,容重平均值为(794.03±21.07)g/L,籽粒硬度指数平均值为(65.82±5.64)%,粗蛋白质含量平均值为(15.51±1.26)%,湿面筋平均值含量为(36.05±4.58)%,面筋指数平均值为(77.08±23.14)%,降落数值平均值为(374.76±46.06)s,沉降值数平均值为(43.21±10.05)mL,吸水率平均值为(61.08±2.98)%,形成时间平均值为(9.77±9.90)min,稳定时间平均值为(16.74±10.76)min,弱化度平均值为(47.08±25.92)BU,最大拉伸阻力平均值为(515.91±220.19)E.U.,延伸度平均值为(165.67±24.65)mm。  相似文献   

18.
Characteristics of carbon dioxide sorption by stored wheat   总被引:1,自引:0,他引:1  
The sorption of carbon dioxide gas (CO2) by wheat was determined in glass flasks at four temperatures (0, 10, 20 and 30 °C) and four moisture contents (m.c.) (12, 14, 16 and 18% wet basis). The gaseous concentrations were analyzed by gas chromatography and the vacuum developed from the sorption of CO2 by wheat was measured with a mercury manometer. The calculated amount of CO2 sorted at equilibrium was a non-linear function of both temperature and moisture content. Sorption of CO2 by wheat decreased with increasing temperature from 0 to 30 °C at 14% m.c., and the initial rate of sorption increased with increasing m.c. from 12 to 18% at a temperature of 20 °C. Sorption was modelled using non-linear regression at two conditions (0–30 °C at 14% moisture content and 12–18% moisture content at 20 °C). The maximum mass of CO2 sorbed in 60 h was 0.510 g/kg of wheat at 18% m.c. and 0 °C and the lowest was 0.224 g/kg at 18% m.c. and 30 °C. A linear relationship existed between the initial CO2 concentration and the concentration after 60 h when 250 g of wheat of 14% m.c. at 20 °C was exposed in 500 ml flasks.  相似文献   

19.
BACKGROUND: Policosanol (PC) is a mixture of high‐molecular‐weight aliphatic primary saturated alcohols which possesses cholesterol‐lowering properties. Literature on PC contents and compositions of wheat varieties is limited. The main objective of this study was to evaluate the effect of genotype and environment on PC content and composition in wheat grain. RESULTS: Grain samples were collected from three varieties, Jagger, Trego and Intrada, grown at three locations, Alva, Balko and Goodwell, OK, in 2005. Two sets of samples were obtained from Goodwell (irrigated and dryland samples). Total PC content and PC composition in whole wheat grain samples were determined using a gas chromatography system. PC contents of the whole wheat grain samples varied from 15.9 to 28.7 mg kg?1. Tricosanol, tetracosanol, hexacosanol, octacosanol and triacontanol were the most abundant PC components. Within each location a significant variety effect was observed. There was also a significant location × variety random effect on PC content. CONCLUSION: A fundamental understanding of compositional variation in wheat grain requires multi‐environment testing of genotypes, perhaps over several years. This study is the first step towards achieving this goal by revealing significant genetic differences in a limited set of genotypes. Copyright © 2008 Society of Chemical Industry  相似文献   

20.
通过采用四种不同蛋白酶对麦胚蛋白分别进行单酶水解、双酶同步水解和分步水解,以水解产物的水解度和抗氧化性为指标,比较研究麦胚蛋白的酶解方法与水解物的抗氧化功能的关系。结果表明:单酶水解时碱性蛋白酶的水解物抗氧化效果最好,DPPH 自由基清除率达到39.74%;双酶分步水解的效果优于双酶同步水解,其中先加碱性蛋白酶后加木瓜蛋白酶效果最好,DPPH 自由基清除率达到45.36%。因此选择先加碱性蛋白酶后加木瓜蛋白酶作为水解麦胚蛋白最佳工艺。  相似文献   

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