Yeast 14-3-3 proteins

14-3-3 proteins form a family of highly conserved proteins which are present in all eukaryotic organisms investigated, often in multiple isoforms, up to 13 in some plants. They interact with more than 200 different, mostly phosphorylated proteins. The molecular consequences of 14-3-3 binding are diverse: this binding may result in stabilization of the active or inactive phosphorylated form of the protein, to a conformational alteration leading to activation or inhibition, to a different subcellular localization, to the interaction with other proteins or to shielding of binding sites. The binding partners, and hence the 14-3-3 proteins, are involved in almost every cellular process and 14-3-3 proteins have been linked to several diseases, such as cancer, Alzheimer's disease, the neurological Miller-Dieker and spinocerebellar ataxia type 1 diseases and bovine spongiform encephalopathy (BSE). The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe both have two genes encoding 14-3-3 proteins, BMH1 and BMH2 and rad24 and rad25, respectively. In these yeasts, 14-3-3 proteins are essential in most laboratory strains. As in higher eukaryotes, yeast 14-3-3 proteins bind to numerous proteins involved in a variety of cellular processes. Recent genome-wide studies on yeast strains with impaired 14-3-3 function support the participation of 14-3-3 proteins in numerous yeast cellular processes. Given the high evolutionary conservation of the 14-3-3 proteins, the experimental accessibility and relative simplicity of yeasts make them excellent model organisms for elucidating the function of the 14-3-3 protein family.     

Investigation of selected toxicological parameters of γ-pentachlorocyclohexene (γ-PCCH)

The toxic effects of the γ-hexachlorocyclohexane metabolite γ-pentachlorocyclohexene (γ-PCCH) were studied by acute and subacute (6 weeks) experiments. The investigations included cerebral convulsibility with chemoshock (Tetrazolium), reactivity with hot plate method, the learning ability with learning tests, and peripheral nervous activity (EMG). Nociceptive reaction time was not influenced, the learning process (6 weeks) was inhibited by γ-PCCH. The conduction velocity of the peripheral nerve was decreased. At the end of the 6th week liver enlargement was found.     

3-苄硫基噻吩、3-糠硫基噻吩和3-十二烷硫基噻吩的合成及其香气特征

-40℃下,3-溴噻吩先与n-丁基锂反应,再依次加入碘、活性镁粉和二硫醚,在室温下分别反应4、4.5、4.5h,分别合成3-苄硫基噻吩、3-糠硫基噻吩和3-十二烷硫基噻吩,收率分别为51.1%、29.0%和27.4%.这些化合物结构都通过IR、MS和~1HNMR测试技术进行了表征,并对其进行了初步香味评价.结果表明,它们都具有基本肉香味特征,阈值为19.78～30.11mg·kg~(-1),留香时间可达2.5～3.5h.     

Part 3

正论坛、公益、科普,微信、微博、抖音……横向观察社会各界在爱眼日期间的主题活动,我们能看出围绕着"普遍的眼健康"这一主题,医院、学校、眼镜企业、行业及协(商)会等是如何开展与自身职能相关、自己最为擅长的活动的。而纵览历年爱眼日的主题,回顾企业和单位每年活动的变化,我们也能动态且辩证地感受到,随着社会生活、行业企业的发展,爱眼日的主题有了显著的变化。     

Cystathionine γ-lyase of Saccharomyces cerevisiae: Structural gene and cystathionine γ-synthase activity

Purification of Saccharomyces cerevisiae cystathionine γ-lyase (γ-CTLase) was hampered by the presence of a protein migrating very close to it in various types of column chromatography. The enzyme and the contaminant were nevertheless separated by polyacrylamide gel electrophoresis. N-terminal amino acid sequence analysis indicated that they are coded for by CYS3(CYI1) and MET17(MET25), respectively, leading to the conclusion that CYS3 is the structural gene for γ-CTLase and that the contaminant is O-acetylserine/O-acetylhomoserine sulfhydrylase (OAS/OAH SHLase). Based on these findings, we purified γ-CTLase by the following strategy: (1) extraction of OAS/OAH SHLase from a CYS3-disrupted strain; (2) preparation of antiserum against it; (3) identification of a strain devoid of the OAS/OAH SHLase protein using this antiserum; and (4) extraction of γ-CTLase from this strain. Purified γ-CTLase had cystathionine γ-synthase (γ-CTSase) activity if O-succinylhomoserine, but not O-acetylhomoserine, was used as substrate. From this notion we discuss the evolutional relationship between S. cerevisiae γ-CTLase and Escherichia coli γ-CTSase.     

问答3则

1 何为直接/间接网版的优缺点? 优点: 直接/间接网版能分辨并印出精细图纹而且墨层厚。有各种厚度的膜片,因此可用于不同目数的丝网。只要操作得当就能获得良好的耐用性。 这种制版方法已流行多年,因为它通过提供优秀的印刷质量、良好的耐用性和多方面的适应     

三维织物的编织技术

本文简要介绍了三维编织技术的发展、特点和三维编织物的性能,三维管状编织物的编织过程。     

Isoflavones reduce inflammation in 3T3-L1 adipocytes

Isoflavones are widely consumed and they have been attributed beneficial effects. We have explored how genistein, daidzein and equol affect the adipocyte functions of glucose uptake and the secretion of inflammatory molecules in 3T3-L1 adipocytes inflamed with TNF-α.     

Ty3/gypsy-like retrotransposons in Candida albicans and Candida dubliniensis: Tca3 and Tcd3

Ty3/gypsy retrotransposons are a widespread group of eukaryote mobile genetic elements. They are similar in structure to, and may be ancestors of, the vertebrate retroviruses. Here we describe the first Ty3/gypsy retrotransposons from the pathogenic yeasts Candida albicans and Candida dubliniensis, which we refer to as Tca3 and Tcd3, respectively. Tca3 was first identified in a variety of strains as an element lacking a large part of its coding region. Comparative analyses between C. albicans and C. dubliniensis allowed us to identify the closely related full-length Tcd3 element, and, subsequently, the full-length Tca3 elements. The full-length versions of Tca3 and Tcd3 are broadly similar in structure to other Ty3/gypsy elements, but have several features of special interest, e.g. both elements appear to have a novel mechanism for priming minus-strand DNA synthesis, probably involving conserved secondary structures adjacent to the 5' LTRs. Also, while closely related to each other, the two elements appear to be fairly distantly related to other known Ty3/gypsy-like elements. Finally, the occurrence of the internally deleted forms of Tca3 in many strains raises interesting questions concerning the evolution of these transposable elements in Candida and the evolution of Candida itself. The sequences reported in this paper have been assigned GenBank Accession Nos AF499463, AF499464 and AF510498.     

神奇3D打印笔开启无限创意

空中涂鸦3D物体缤纷呈现在经过一年多的技术研发之后,英国WobbleWorks玩具公司最近生产出世界上第一款具有3D打印功能的3Doodler笔。笔的长度180毫米,直径24毫米,重量200克左右,使用万能电源,工作条件为110V或240V。如同3D打印机一样,它也用ABS或PLA塑料当作“墨水”。每只3D涂鸦笔都配备一包这样的“墨水”,里面有10只300毫米长的塑料,每只可创作大约3.4米的3D作品。ABS是最常见的塑料之一,用于很多塑料制品。“生物塑料”PLA来自玉米,可降解并且熔点比ABS要低。从笔头喷出的塑料虽然可接触,但由于笔尖处温度高达270℃,因此使用时一定不要触碰笔尖。     

简讯3则

2010年1月20日，中国机床工具工业协会六届二次常务理事（扩大）会议在武汉召开，工业和信息化部装备工业司司长张相木、国家能源节约和科技装备司司长李冶、国家发展和改革委员会宏观经济研究院副院长陈东琪、武汉市委常委贾耀斌出席会议并致辞或做重要讲话；武汉市副市长文振富、工业和信息化部装备工业司王建宇处长、国家能源局能源节约和科技装备司王书强处长等出席了会议；