反胶束结构的研究进展

反胶束是光学透明的溶液,主要介绍了反胶束的结构参数如胶束形状、尺寸、聚集数等;以及反胶束结构的研究方法,动力学模拟、核磁共振及光谱学方法,以期为反胶束的研究提供参考。     

反胶束萃取大豆油脂及其与浸出法的比较研究

采用超声辅助2种反胶束体系萃取大豆油脂,分析超声波技术影响反胶束萃取大豆油脂的主要因素,并分析所得油脂的品质与之浸出法进行了对比.经试验研究得到,最佳的工艺条件为超声时间lh、超声温度60℃、超声功率300 W、全脂豆粉加入量为0.04 g/mL;2种不同反胶束体系萃取大豆油脂的萃取率差别不大,AOT反胶束法的前萃率为81.39％,AOT-Tween85反胶束法的前萃率为84.17％.反胶束萃取得到的油脂,酸价及过氧化值比浸出法要低;浸出法、AOT反胶束法、AOT-Tween85反胶束法3种方法提取的大豆油脂脂肪酸组成和含量基本相同,色泽、皂化值也基本相同,碘值稍有差异.     

反胶束萃取技术及其在食品中的应用

综述了反胶束萃取技术在食品中的应用,包括反胶束萃取技术分离应用研究和应用于酶的催化研究,并指出了此种方法具有良好的食品工业应用前景.     

反胶束萃取氨基酸的研究进展

综述了反胶束萃取氨基酸的研究进展,包括萃取机理、应用研究的进展。利用反胶束技术萃取氨基酸的研究显示了良好的工业化应用前景。     

反胶束萃取植物蛋白质结构与功能特性研究进展

该文综述国内外关于反胶束萃取技术分离得到的植物蛋白质结构与特性研究进展,阐述了反胶束萃取对植物蛋白质分子构象、微观结构、亚基构成、氨基酸组成的影响,以及结构改变引起蛋白质功能特性的差异;并提出反胶束萃取技术存在的问题及前景展望。     

AOT与SDS复合反胶束体系的研究

研究丁二酸二异辛酯磺酸钠(AOT)与十二烷基硫酸钠(SDS)复合反胶束的体系特征,并与AOT、SDS单一反胶束体系进行对比。结果表明,随着表面活性剂浓度的升高,3种反胶束体系WO逐渐下降,增比黏度逐渐上升,电导率基本没有变化;随着加水量的增加,3种反胶束体系WO、增比黏度以及电导率都逐渐升高。AOT与SDS复合反胶束可以加溶更多的水,且体系较为稳定,这为AOT与SDS复合反胶束体系萃取技术的进一步研究提供了可靠的理论依据。     

反胶束萃取技术及其在植物蛋白质提取中的应用研究进展

反胶束萃取是一种适合生物大分子分离纯化的新型技术,在生物活性物质的萃取上有着广泛的应用前景.文章阐述反胶束萃取的含义、反胶束萃取蛋白质机理,并对近年来国内外有关反胶束萃取在植物蛋白质提取中的应用研究进行综述,对该领域目前存在的问题进行评述和展望.     

反胶束后萃蛋白质研究进展

反胶束是表面活性剂分子在非极性有机溶剂中自发形成的具有热力学稳定性的纳米级聚集体,反胶束萃取可以分为前萃和后萃2个过程。文章综述近年来国内外关于后萃过程的动力学研究以及后萃蛋白质优化的方法,为今后反胶束后萃技术的研究提供理论依据。     

反胶束体系中酶催化技术在食品科学中的研究进展

该文就酶催化技术以反胶束体系为介质在食品中的应用进行了阐述。介绍了酶催化在反胶束体系中的反应类型、增溶方法以及模型;简述不同表面活性剂制备成的反胶束体系中,酶催化技术在食品领域的应用,并对酶催化技术在反胶束体系中的应用进行展望。     

反胶束萃取氨基酸的研究进展

综述了反胶束萃取氨基酸的研究进展，包括萃取机理、应用研究的进展。利用反胶束技术萃取氨基酸的研究显示了良好的工业化应用前景。     

Extraction and physicochemical properties of soya bean protein and oil by a new reverse micelle system compared with other extraction methods

Reverse micelle extraction is a novel technology for the separation of plant components such as proteins and oil. In this study, sodium bis(2‐ethylhexyl) sulphosuccinate (AOT) reverse micelle system and AOT/Tween 85 reverse micelle system were used to extract soya bean protein and oil from soya bean flour. The physicochemical properties of the protein and oil extracted were investigated and compared with traditional extraction methods. The results showed that the efficiency of forward extraction of soya bean protein using an AOT/Tween 85 reverse micelle system was superior to that using an AOT reverse micelle system at the optimal extraction conditions. In addition, soya bean proteins extracted using reverse micelle extraction had no unordered structure under Fourier transform infrared spectroscopy. The acid and peroxide values of oil products from two reverse micelle extractions were lower than that from immersion. The results indicated that AOT/Tween 85 reverse micelle system is effective in extracting soya bean protein and oil.     

反胶束萃取法在分析植物油中钾钠离子的应用

通过制备反胶束萃取溶液,选择性地将植物油中钾钠离子螯合进入反胶束胶团中并进行萃取与反萃取,实现快速分离富集植物油中钾钠离子目的。通过反胶束溶液制备及其萃取试验发现,以硝基苯为溶剂和正辛醇为分散剂配制浓度为7 mmol/L螯合剂18-冠醚-6的反胶束溶液,加入6.0 mL反胶束溶液于15.00 mL的植物油样品中,加入5.0 mL氨-氯化氨缓冲溶液,在室温条件下,置于超声频率40 kHz、超声时间10 min可使钾钠离子从植物油样品快速萃取到无机相以达到仪器分析要求,然后用原子吸收分光光度计进行定量测定,从而建立一种从植物油中有效分离富集钾钠元素的方法。     

不同反胶束体系后萃取花生蛋白质的比较

对AOT[二-(2-乙基己基)琥珀酸酯磺酸钠]/异辛烷,SDS(十二烷基硫酸钠)/异辛烷-正辛醇,DTAC(十二烷基三甲基氯化铵)/正庚烷-正己醇3种反胶束体系萃取花生蛋白质的后萃工艺进行研究.主要研究了缓冲溶液pH值、萃取时间、萃取温度、超声功率、KCl浓度对花生蛋白后萃率的影响,分别得到了3种反胶束体系萃取花生蛋白质的最佳后萃工艺条件,并做验证试验.在最优工艺条件下制备不同的花生蛋白样品.通过色差分析,从宏观上比较不同反胶束体系制备的花生蛋白产品色泽的差异,进一步对比不同反胶束体系制备的花生蛋白的扫描电镜(SEM)照片,分析其微观结构的差别,试验结果表明最适合萃取花生蛋白的反胶束体系是AOT反胶束体系,且该体系萃取花生蛋白的后萃率为83.17％,较另外2种体系的后萃率都高.     

Kinetics of Protein Extraction in Reverse Micelle

Sodium bis (2-ethylhexyl) sulfosuccinate (AOT)-sodium dodecyl sulfate (SDS)/isooctane-octanol reverse micelle extraction was tested an efficient and effective approach to separate peanut protein from full-fat peanut powder. Here, important kinetic factors including pH, ion strength, and temperature were studied during reverse micelle backward extraction. The extraction conditions were obtained by response surface experiments as follows: pH 7.5, ion concentration 1.1 mol/L at temperature 35°C. Under these optimum extraction conditions, the extraction rate of protein reached 79.03%. A model on the kinetic partitioning of peanut protein was also developed. The backward extraction in this reverse micelle system was controlled by interfacial resistance instead of diffusion resistance in reverse micelle and aqueous phase with the total mass transfer rate of 0.8×10^?5 m³·s^?1. A two-film theory may be the mechanism for flat interface. Results of mass transfer process are helpful for creating an reverse micelle extraction process, and used for purification of peanut proteins, promoting the development of food industry.     

Reverse micellar extraction of bovine serum albumin – A comparison between the effects of gemini surfactant and its corresponding monomeric surfactant

Gemini surfactant displayed distinct advantages over monomeric surfactant in the liquid–liquid reverse micellar extraction process. First, less amount of gemini surfactant than monomeric surfactant was needed for transferring almost complete bovine serum albumin (BSA) into organic phase from aqueous phase. Second, the loading capacity of gemini surfactant reverse micelle phase was much higher than that of the corresponding monomeric surfactant reverse micelle. Third, efficient backward extraction (75–92%) of BSA could be effected in a wide pH range from 4 to 9 with gemini surfactant reverse micelle while a pH of ca. 4.3 is prerequisite to the recovery of BSA from monomeric surfactant reverse micelle. So far, the reports about the effect of surfactant structure on protein extraction have been limited. This study indicates the important role of the spacer of gemini surfactant in protein extraction process and may provide more knowledge on how to optimise surfactant structure.     

反胶束对植物蛋白的结构、物化特性、功能性和应用的影响研究进展

反胶束提取法是一种适用于生物大分子分离纯化的新技术,在植物蛋白提取方面具有广阔的应用前景。本文介绍了国内外反胶束法提取和纯化植物蛋白的研究进展,阐述了反胶束体系的定义以及其提取蛋白质的原理和特点,并比较和总结了反胶束法和传统方法制备的植物蛋白结构和物化性质的差异,讨论了反胶束萃取法对植物蛋白的结构、物化、功能性和应用的影响,提出了反胶束萃取技术应用到工业生产中面临的主要难题,并根据提取特点展望其应用前景。     

反胶团提取大豆中的蛋白质和豆油

本文进行了由丁二酸-（二）-2-乙基乙基酯磺酸钠（AOT）-正乙烷-氯化钾组成的反胶团体系从大豆中萃取蛋白质和豆油的研究，考查了大豆和反胶团质量体积比、萃取时间、温度、pH值和盐浓度等因素对蛋白质的萃取及反萃取的影响。结果表明：大豆蛋白质的萃取率最高达96.9%，豆油的萃取率为90.5%。     

Effect of reverse micelle on conformation of soy globulins: A Raman study

Raman spectroscopy was applied to investigate the main and side chain conformational changes of 7S and 11S globulins from soybean proteins using aqueous buffer and reverse micelle extraction. The 7S and 11S globulins using two extraction methods displayed typical spectral features (such as amide I, III region and the side chain conformations of particular residues) due to characteristic amino acid composition and molecular conformation. In comparison, the degree of molecular disorder increased in both globulins using reverse micelle extraction and new bands appeared. The relative amount of different structures of 7S and 11S globulins could be estimated through accurate measurement of the band intensities. Finally, the increase of the I_850/830 intensity ratio of Raman tyrosine doublet in 11S globulin with reverse micelle extraction suggested a change towards a more exposed state of tyrosine residues, in good agreement with the more disordered conformation taken upon reverse micelle.     

P204/正辛醇反胶束萃取L-辛弗林的研究

目的建立一种反胶束萃取L-辛弗林的方法,并验证利用P204/正辛醇萃取L-辛弗林的有效性。方法制备P204/正辛醇反胶束体系萃取枳实L-辛弗林,反萃取液经浓缩干燥后,获得纯化L-辛弗林。利用基于单因素实验进行的正交实验研究最佳分离工艺,通过紫外分析、原子力显微镜表征证实了P204/正辛醇反胶束体系萃取分离L-辛弗林的有效性。结果萃取最佳工艺条件为:水相pH值6.5、萃取时间15 min、P204浓度0.10mol/L、W_0=5,此条件下L-辛弗林的单次萃取率为68.02%,各影响因素影响顺序为:水相pHP204浓度W_0萃取时间。纯化后的L-辛弗林纯度达到87.8%,比原料纯度提升了2.9倍。结论该方法为L-辛弗林的分离提取提供一种快速低成本方法,并对其萃取机制及主要变化规律方面提供了基础数据。