In Silico Analysis and Experimental Validation of Active Compounds from Cichorium intybus L. Ameliorating Liver Injury

This study aimed at investigating the possible mechanisms of hepatic protective activity of Cichorium intybus L. (chicory) in acute liver injury. Pathological observation, reactive oxygen species (ROS) detection and measurements of biochemical indexes on mouse models proved hepatic protective effect of Cichorium intybus L. Identification of active compounds in Cichorium intybus L. was executed through several methods including ultra performance liquid chromatography/time of flight mass spectrometry (UPLC-TOF-MS). Similarity ensemble approach (SEA) docking, molecular modeling, molecular docking, and molecular dynamics (MD) simulation were applied in this study to explore possible mechanisms of the hepato-protective potential of Cichorium intybus L. We then analyzed the chemical composition of Cichorium intybus L., and found their key targets. Furthermore, in vitro cytological examination and western blot were used for validating the efficacy of the selected compounds. In silico analysis and western blot together demonstrated that selected compound 10 in Cichorium intybus L. targeted Akt-1 in hepatocytes. Besides, compound 13 targeted both caspase-1 and Akt-1. These small compounds may ameliorate liver injury by acting on their targets, which are related to apoptosis or autophagy. The conclusions above may shed light on the complex molecular mechanisms of Cichorium intybus L. acting on hepatocytes and ameliorating liver injury.     

基于普鲁士蓝/聚多巴胺/纳米金纳米粒子构建的电流型免疫传感器

首先制备普鲁士蓝纳米粒子(PB),通过利用多巴胺(DA)自氧化聚合形成聚多巴胺膜(Dopa)的特性,在PB纳米粒子表面包覆成膜,有效增加PB的稳定性,并在氯金酸溶液中原位还原沉积金纳米粒子,利用纳米金固定化甲胎蛋白抗体(anti-AFP),制得灵敏度高和稳定性好的无标记免疫传感器。采用透射电子显微镜(TEM)对纳米粒子进行表征,采用循环伏安法(CV)和交流阻抗法(EIS)考察修饰电极的电化学特性,实验结果表明,PB-Dopa-Au纳米粒子修饰的电极在实验过程中呈现出良好的氧化还原活性,对甲胎蛋白的检测范围为0.02~80 ng/mL检出限为0.01ng/mL。     

超临界CO_2萃取与浸渍法提取白刺果油化学成分的分析与比较

为了促进白刺属植物在医药和精细化工等领域中的应用,该研究采用超临界CO2萃取法和溶剂浸渍法提取唐古特白刺果油,并用气相色谱-质谱联用技术对其化学成分进行了分析,从超临界CO2萃取出的白刺果油中分离鉴定出51种化学成分;从浸渍法提取出的白刺果油中鉴定出22种成分;两种方法提取的果油共有14种相同化学成分,含量较高的主要有:二十九烷、二十七烷、亚油酸乙酯、γ-生育酚、VE(α-生育酚)、γ-谷甾醇、菜油甾醇、等。该研究工作的新颖性,已为新疆维吾尔自治区科委石河子大学图书馆2007年1月15日出具的第20070000000005号《科技查新报告》所证实。     

3种红花的体外抑菌作用研究

研究新红4号、新红7号、云红2号3种红花的抑菌作用。分别采用水提法和醇取法提取红花有效成分,以大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌、八叠球菌、铜绿假单胞菌为供试菌,利用滤纸片扩散法测定3种红花的水提物和醇提物的抑菌作用,并用2倍稀释法测定最小抑菌浓度(MIC)。醇提物中云红2号醇提物对铜绿假单胞菌的抑制作用较明显、MIC为62.5 mg/m L;水提物中新红4号水提物对5种菌的抑制作用都很明显、其对大肠杆菌的MIC为62.5 mg/m L,新红7号水提物对除金黄色葡萄球菌之外的4种菌有很好的抑制作用、其对大肠杆菌的MIC为125mg/m L。     

新疆西伯利亚白刺果中红色素的提取工艺

以溶剂浓度、浸提时间、pH值为因素，各设3个水平，按L9（34）正交表设计实验，筛选白刺红色素的最佳提取工艺。结果得出采用pH1的20％乙醇，提取4次，每次0．5h效果较佳。本方法工艺简便、合理可行，适于工业化生产。     

棉油皂脚中植物甾醇的提取工艺研究

为了探索从棉油皂脚中提取植物甾醇的工艺条件。以皂脚为原料,通过单因素和正交实验优化最佳工艺条件,并对粗品进行纯化,以高效液相色谱法测定的甾醇含量为考察指标。植物甾醇提取的最佳工艺为:皂化料液比1∶3,90℃下皂化3 h,用乙酸乙酯进行萃取,料液比为1∶20,粗甾醇的提取率为75.6%,其中提取得到的β-谷甾醇和菜油甾醇的纯度分别为(66.5±0.47)%、(5.67±0.08)%,其经无水乙醇重结晶两次后,纯度可达91.8%。结果表明最佳提取工艺重现性较好;HPLC法测定甾醇含量结果准确可靠。     

沙枣中鞣质的超声提取及含量测定

寻找在超声条件下从沙枣中提取鞣质的优化条件,并进行含量测定。在单因素试验的基础上,采取正交设计法,以料液比,丙酮浓度,提取温度,超声时间为因素,每个因素3个水平,选择L9(34)正交表进行实验,用络合滴定法测定鞣质含量作为综合评价指标。最佳提取条件:料液比为1∶10,丙酮浓度为60%,提取温度为35℃,超声时间为35 min。最佳条件提取的鞣质含量为9.874 3%。超声法提取沙枣鞣质操作简单,效果好而且节省时间。     

Assessment of the gluten toxicity of wheat and naan in Xinjiang Uyghur Autonomous Region,China

Xinjiang is a high-risk area for celiac disease (CD) regardless of genetic or environmental factors. However, no case has been reported yet in people living in Xinjiang. This study aims to explore the potential connection between diet and occurrence of CD in the Xinjiang population. To this end, the levels of T-cell stimulatory epitopes in 164 accessions of Xinjiang wheat were tested by using Western blot with monoclonal antibodies against α-gliadin epitopes Glia-α9 and Glia-α20. Three wheat varieties with remarkably low amounts of T-cell stimulatory epitopes were obtained. Western blot and R5 competitive ELISA were performed for the assessment of potential toxicity related to CD of naan. Results showed a reduction of gluten toxicity after wheat flour was processed into naan, suggesting it may have the potential to help to reduce the risk of CD for the genetically predisposed individuals.     

环磷酸腺苷颗粒剂的制备

目的:优化从红枣中提取环磷酸腺苷(cAMP)的提取工艺,选择合适的辅料优化cAMP颗粒剂的成型工艺。方法:以cAMP的提取率为评价指标,对料液比、提取时间、乙醇体积分数、微波功率及微波处理时间采用响应面法进行优化,对可溶性淀粉、乳糖、糊精及其混合物的成型性、堆密度、休止角、溶化率及吸湿性等评价指标进行综合评分,选出合适的颗粒剂辅料,再以颗粒剂成型性为评价指标,通过正交实验设计选择最优的辅料比、乙醇体积分数及颗粒剂干燥温度,确定颗粒剂成型工艺并进行工艺验证。结果:cAMP提取工艺最优条件为:料液比1:15(g/mL),提取时间7.5 h,微波功率270 W,乙醇体积分数30%,微波处理时间10 min,在此条件下,cAMP提取率403.6 μg/g;颗粒剂最优辅料配比为浸膏:乳糖为1:4,乙醇体积分数为75%,干燥温度50℃。结论:此方法可行,重复性好,能制备出较好的颗粒剂,能有效的控制环磷酸腺苷颗粒的质量。     

迷迭香酸对胰岛素硝化的抑制作用

目的：采用化学体系考察迷迭香酸（rosmarinic acid,RA）对胰岛素硝化的保护作用。方法：采用比色法和非变性聚丙烯酰胺凝胶电泳检测迷迭香酸对胰岛素和过量过氧亚硝基阴离子（ONOO－）反应的影响。结果：胰岛素可以和ONOO－反应生成硝化胰岛素,在396 nm波长处有最大吸收峰。RA可浓度依赖性地抑制ONOO－所致的胰岛素硝化作用,但对已经硝化的胰岛素没有影响。结论：RA通过清除ONOO－抑制胰岛素的硝化作用。