The Role of Chronic Inflammation in Various Diseases and Anti-inflammatory Therapies Containing Natural Products

Chronic inflammation represents a long-term reaction of the body's immune system to noxious stimuli. Such a sustained inflammatory response sometimes results in lasting damage to healthy tissues and organs. In fact, chronic inflammation is implicated in the development and progression of various diseases, including cardiovascular diseases, respiratory diseases, metabolic diseases, neurodegenerative diseases, and even cancers. Targeting nonresolving inflammation thus provides new opportunities for treating relevant diseases. In this review, we will go over several chronic inflammation-associated diseases first with emphasis on the role of inflammation in their pathogenesis. Then, we will summarize a number of natural products that exhibit therapeutic effects against those diseases by acting on different markers in the inflammatory response. We envision that natural products will remain a rich resource for the discovery of new drugs treating diseases associated with chronic inflammation.     

基于IP SAN的高校数据中心网络存储系统设计

网络存储技术是当前存储应用的发展趋势,IP SAN是其中的热点之一,如何构建网络存储系统直接关系到校园网络的安全性。介绍了SAN、ISCSI协议及其工作原理,结合高校数据中心存储的需求分析,提出了一种基于ISCSI协议的经济实用、高性价比的高校数据中心网络存储设计方案。     

米炒党参炮制过程中党参炔苷、5-HMF和党参多糖含量动态变化研究

目的：研究不同米炒党参在炮制过程中党参炔苷、5-HMF和党参多糖的动态变化。方法：高效液相色谱法测定党参炔苷、5-HMF含量,紫外-可见分光光度法测定党参多糖含量动态变化过程。结果：（1）党参炔苷：随着炒制时间的延长,党参炔苷含量均呈先上升后下降趋势;在相同时间间隔点,小米炒党参炔苷含量较高。（2）5-HMF：随着炒制时间的延长,5-HMF含量均呈显著上升趋势;在相同时间间隔点,小米炒党参5-HMF含量较低。（3）党参多糖：随着炒制时间的延长,党参多糖含量均呈先上升后下降趋势;在相同的时间间隔点,炒制时间为5.5min之前的样品中小米炒党参多糖含量均高于大米炒党参,炒制时间为5.5min之后的样品中小米炒党参多糖含量均略低于大米炒党参。结论：党参分别采用小米、大米炒制后,3种成分均发生了显著变化,但小米炒党参和大米炒党参变化趋势不一致。     

中华稻蝗内生菌株SDLH抗菌肽的分离纯化

采用PEG 6000沉淀法、DEAE-32阴离子层析和Sephadex G-50凝胶层析分离纯化SDLH抗菌肽,抑菌圈法测定抑菌活性,Bradford法测定蛋白含量,PAGE检测纯度。结果获得分子量为17.0 KD,电泳纯的抗菌肽。     

连翘叶风味爆珠的制备工艺研究

利用冷冻反向成球技术,即将乳酸钙和黄原胶复配成芯液后,冷冻为小球,然后置于常温放置的海藻酸钠-低酯果胶复合溶液中,蒸馏水漂洗后用壳聚糖溶液固化,制得爆珠。以跌落测试、质构分析作为主要评判标准,并观察小球的成球性,得到爆珠的最佳制作工艺。为丰富爆珠的营养价值和口感,将具有药食两用价值的连翘叶提取物加入爆珠芯液中,以感官评分为标准,通过单因素试验和正交试验得到连翘叶风味爆珠的最佳制备工艺。试验结果表明,爆珠的最佳制备工艺为:成球反应时间10 min、海藻酸钠-低酯果胶复合溶液浓度0.6%(w/w)、芯液添加量400 μL;连翘叶风味爆珠芯液的最佳工艺配方为:连翘叶提取物载入量3%(w/w)、白砂糖载入量8%(w/w)、黄原胶载入量0.2%(w/w)。该结果可为连翘叶的开发利用提供科学依据,为风味爆珠的研发提供工艺参考。     

Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody

Competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of porcine gelatin in edible bird's nest (EBN). Three ELISAs were developed by using polyclonal rabbit antibodies against porcine species-specific amino acid sequences of collagen α2 (I) chain (pAb1 and pAb2) and α1 (I) chain (pAb3). The limit of detection (IC₁₅) of the three ELISAs was 0.033, 0.082 and 0.052 μg/mL respectively. The median inhibitory concentration (IC₅₀) of pAb1, pAb2 and pAb3 was 0.265, 0.394 and 0.228 μg/mL respectively, as well as able to recognise porcine and bovine gelatins. pAb1 showed slight cross-reactivity with cave nest and egg white, while pAb2 exhibited slight cross-reactivity with blood cave nest and egg white. No cross-reactivity was observed with EBNs and egg white for pAb3. The recoveries of porcine gelatin spiked EBNs were in the range of 62.8–125.4% with intra- and inter-day coefficient of variants (CVs) of 2.9–5.4% and 4.7–9.6% respectively when using pAb3. Taking into account all abovementioned factors, pAb3 appeared sufficient for EBN authentication.     

百合中二氧化硫脱除方法的初步研究

目的：初步研究浸渍法、微波法、超声波法脱除百合中二氧化硫的效果。方法：在不同温度下,采用浸渍法、超声波处理百合;在不同微波功率下,脱除百合中残留的二氧化硫,用标准碘液滴定测定二氧化硫的量。结果：百合中二氧化硫的含量高达1 869 mg/kg。255 kW微波处理对药材中二氧化硫的脱除有较好效果,最大脱除率可达72.93%。结论：255 kW微波处理可作为百合脱硫的一种切实有效的方法。     

Review of the structural characterization,quality evaluation,and industrial application of Lycium barbarum polysaccharides

BackgroundLycium barbarum polysaccharides (LBPs) are considered to be the major bioactive components in L. barbarum. Due to the considerable health benefits of LBPs, and the significant research interest in LBPs, quality evaluation of LBPs and their related products is imperative for ensuring their efficacy and safety. The bioactivities of polysaccharides are closely associated with their physicochemical properties, which can be easily monitored via chemical techniques. Therefore, analysis of physicochemical properties is a more straightforward and viable approach for the quality control of polysaccharides.Scope and approachIn this review, special techniques for the structural characterization of LBPs are summarized and discussed. In addition, quality evaluation approaches of LBPs that have never been emphasized are highlighted herein. Furthermore, industrial applications of LBPs are reviewed and discussed.Key findings and conclusionsHigh-performance anion-exchange chromatography coupled with pulsed amperometric detection is one of the most effective techniques for analysis of both acidic and neutral LBPs. Size-exclusion chromatography coupled with multi-angle laser light scattering and refractive index detection is able to determine absolute molecular weights, chain conformations, and contents of LBPs. Fingerprinting analysis and saccharide mapping analysis are effective approaches for quality evaluation of LBPs. However, the lack of appropriate quality evaluation approaches indicates an unreliable regulation of LBPs and their related products. This reveals the deficiency in the quality control of commercial products. Thus, the development of reliable approaches for pharmacological activity-based quality control of LBPs and their related products must be extensively investigated.     

糖尿病患者肠道中4 种重要菌群的定量分析

目的：了解正常人群与Ⅱ型糖尿病人,以及Ⅱ型糖尿病人在是否有家族遗传、是否用药治疗和是否伴随肥胖等情况下肠道中拟杆菌、双歧杆菌、柔嫩梭菌和乳杆菌的含量与变化,为预防和治疗Ⅱ型糖尿病开辟新途径。方法：收集上述各类人群粪便样品（每组10 人）,分别提取样品中微生物的总DNA,运用荧光定量聚合酶链式反应（polymerase chain reaction,PCR）扩增4 种重要菌群的特异性片段,计算含量,分析上述菌群的变化差异。结果：正常人群肠道中的拟杆菌和双歧杆菌含量均显著低于Ⅱ型糖尿病人群;柔嫩梭菌和乳杆菌含量均显著高于后者。在Ⅱ型糖尿病人群中,用药患者组和肥胖＋高血糖患者组的肠道中拟杆菌和双歧杆菌含量均显著低于未用药患者组和单纯高血糖患者组,而柔嫩梭菌和乳杆菌含量均显著高于后者。有无Ⅱ型糖尿病遗传史对患者肠道内4 种菌群的含量均无显著影响。结论：4 种重要菌群与Ⅱ型糖尿病的发生和发展有密切关系;用药治疗后的患者肠道中4 种重要菌群含量比其他各组更接近正常人群;肥胖易加重Ⅱ型糖尿病病情;而有无Ⅱ型糖尿病家族遗传对这4 种菌群的含量几乎没有影响。     

药食同源中药抗衰老研究进展

"药食同源"即"医食同源,药食同根",药食同源中药兼具了丰富的营养价值和药用价值。近年来,对药食同源中药进行抗衰老研究的报道越来越多,药食同源中药所具有的抗衰老活性也逐渐被人们所认知,因此将其开发成抗衰老药物具有广阔的发展前景。本文以药食同源中药为研究对象,通过其抗氧化活性以及已报道药食同源中药抗衰老研究的文献数量,筛选出可以用作抗衰老研究的中药;同时,以衰老机制为基础,对药食同源中药的研究进行综述,并对其今后的研究方向进行展望。