全文获取类型
收费全文 | 13842篇 |
免费 | 1645篇 |
国内免费 | 792篇 |
专业分类
电工技术 | 1408篇 |
技术理论 | 1篇 |
综合类 | 1707篇 |
化学工业 | 859篇 |
金属工艺 | 157篇 |
机械仪表 | 349篇 |
建筑科学 | 4956篇 |
矿业工程 | 1440篇 |
能源动力 | 390篇 |
轻工业 | 311篇 |
水利工程 | 838篇 |
石油天然气 | 427篇 |
武器工业 | 152篇 |
无线电 | 1045篇 |
一般工业技术 | 805篇 |
冶金工业 | 359篇 |
原子能技术 | 87篇 |
自动化技术 | 988篇 |
出版年
2024年 | 63篇 |
2023年 | 175篇 |
2022年 | 357篇 |
2021年 | 415篇 |
2020年 | 428篇 |
2019年 | 318篇 |
2018年 | 318篇 |
2017年 | 382篇 |
2016年 | 456篇 |
2015年 | 491篇 |
2014年 | 1063篇 |
2013年 | 820篇 |
2012年 | 1086篇 |
2011年 | 1111篇 |
2010年 | 903篇 |
2009年 | 927篇 |
2008年 | 882篇 |
2007年 | 992篇 |
2006年 | 838篇 |
2005年 | 770篇 |
2004年 | 620篇 |
2003年 | 537篇 |
2002年 | 447篇 |
2001年 | 395篇 |
2000年 | 363篇 |
1999年 | 255篇 |
1998年 | 179篇 |
1997年 | 142篇 |
1996年 | 107篇 |
1995年 | 104篇 |
1994年 | 86篇 |
1993年 | 60篇 |
1992年 | 40篇 |
1991年 | 41篇 |
1990年 | 23篇 |
1989年 | 20篇 |
1988年 | 16篇 |
1987年 | 13篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1984年 | 8篇 |
1983年 | 1篇 |
1982年 | 5篇 |
1980年 | 2篇 |
1979年 | 8篇 |
1970年 | 1篇 |
1962年 | 1篇 |
1959年 | 2篇 |
1955年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 42 毫秒
1.
以从自然腐败的樱桃上分离的链格孢霉(Alternaria sp.)LD3.0086为指示菌,研究苯乳酸对链格孢霉的主要抑制作用靶位。应用分光光度法测定苯乳酸对链格孢霉的最小抑菌浓度,通过卡尔科弗卢尔荧光增白剂染液(calcofluor white,CFW)染色观察苯乳酸对菌丝顶端生长的破坏作用,利用扫描电子显微镜和透射电子显微镜观察链格孢霉的超微结构变化,通过测定苯乳酸作用前后链格孢霉上清液中N-乙酰葡萄糖胺质量浓度变化研究苯乳酸对菌丝细胞壁的破坏作用,应用荧光双染色法观察苯乳酸对链格孢霉菌丝细胞膜的损伤作用。结果表明,12.5 mmol/L的苯乳酸能有效抑制链格孢霉的生长;与对照组(无菌水处理)相比,苯乳酸处理后链格孢霉顶端生长细胞无明显形变,经12.5 mmol/L苯乳酸处理的链格孢霉上清液中N-乙酰葡萄糖胺质量浓度基本不变;苯乳酸处理24 h,链格孢霉菌丝细胞壁表面无明显损伤,细胞内结构发生明显变化;苯乳酸短时间(4 h)处理链格孢霉,菌丝细胞膜仍较为完整,加入苯乳酸较长时间(8 h)后细胞膜发生破裂。综合分析可知,苯乳酸对链格孢霉的主要作用靶位应不是菌丝体的细胞壁和细胞膜,而是在菌丝体内部,通过破坏菌丝内部细胞器结构或引起细胞内的生化反应,从而抑制链格孢霉的生长和繁殖,发挥抑菌活性。 相似文献
2.
Mri Miczi Mria Golda Balzs Kunkli Tibor Nagy Jzsef Tzsr Jnos Andrs Mtyn 《International journal of molecular sciences》2020,21(24)
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His6-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2. 相似文献
3.
Jonathan K. Dozier Mark D. Distefano 《International journal of molecular sciences》2015,16(10):25831-25864
The use of proteins as therapeutics has a long history and is becoming ever more common in modern medicine. While the number of protein-based drugs is growing every year, significant problems still remain with their use. Among these problems are rapid degradation and excretion from patients, thus requiring frequent dosing, which in turn increases the chances for an immunological response as well as increasing the cost of therapy. One of the main strategies to alleviate these problems is to link a polyethylene glycol (PEG) group to the protein of interest. This process, called PEGylation, has grown dramatically in recent years resulting in several approved drugs. Installing a single PEG chain at a defined site in a protein is challenging. Recently, there is has been considerable research into various methods for the site-specific PEGylation of proteins. This review seeks to summarize that work and provide background and context for how site-specific PEGylation is performed. After introducing the topic of site-specific PEGylation, recent developments using chemical methods are described. That is followed by a more extensive discussion of bioorthogonal reactions and enzymatic labeling. 相似文献
4.
Although previous research has investigated widespread use of social media, especially Facebook, by youth attending college, the conditions under which these media foster adjustment to college remain unclear. This study tested a model illuminating pathways linking social competence to college adjustment via students’ perceptions about the usefulness of Facebook and ways in which they used the medium. Self-report survey data from 321 college students (M age = 20.09; 58% female; 84% Caucasian) attending a major Midwestern university supported the proposed model, indicating that higher social competence could foster or impede college adjustment, depending upon how it was related to beliefs about the usefulness of different Facebook functions and how these perceptions, in turn, were associated with patterns of Facebook use. Findings underscore the importance of considering connections among personal attributes, perception of media effectiveness, and media behaviors in assessing the implications of social media for users’ psychosocial well-being. 相似文献
5.
基于传统AI-EBG结构,提出了一种小尺寸的增强型电磁带隙结构,实现了从0.5~9.4 GHz的宽频带-40 dB噪声抑制深度,且下截止频率减少到数百MHz,可有效抑制多层PCB板间地弹噪声。文中同时研究了EBG结构在高速电路应用时的信号完整性问题,使用差分信号方案可改善信号完整性。 相似文献
6.
Dong‐Wook Kim 《ETRI Journal》2006,28(1):84-86
This letter presents a small‐sized, high‐power single‐pole double‐throw (SPDT) switch with defected ground structure (DGS) for wireless broadband Internet application. To reduce the circuit size by using a slow‐wave characteristic, the DGS is used for the quarter‐wave (°/4) transmission line of the switch. To secure a high degree of isolation, the switch with DGS is composed of shunt‐connected PIN diodes. It shows an insertion loss of 0.8 dB, an isolation of 50 dB or more, and power capability of at least 50 W at 2.3 GHz. The switch shows very similar performance to the conventional shunt‐type switch, but the circuit size is reduced by about 50% simply with the use of DGS patterns. 相似文献
7.
8.
连续波半主动雷达制导空空导弹截获跟踪距离计算 总被引:1,自引:1,他引:0
分析了连续波半主动雷达制导导弹导引头工作电磁环境和对武器系统攻击区的影响,建立了热噪声和杂波干扰环境下导引头截获距离方程,分析推导了直波泄漏干扰,地杂波干扰的计算公式,并在此基础上给出了导弹允许攻击区计算公式。 相似文献
9.
介绍了观音阁坝被埋水下6年越冬停浇面水平裂缝采用“粘贴T1密封胶及表面粘三元乙丙后锚固”的处理方案。结果表明,利用胶性材料处理运行中的重力坝迎水面裂缝,技术可靠,方法简单,经济合理。 相似文献
10.