The Age-Sensitive Efficacy of Calorie Restriction on Mitochondrial Biogenesis and mtDNA Damage in Rat Liver

Calorie restriction (CR) is the most efficacious treatment to delay the onset of age-related changes such as mitochondrial dysfunction. However, the sensitivity of mitochondrial markers to CR and the age-related boundaries of CR efficacy are not fully elucidated. We used liver samples from ad libitum-fed (AL) rats divided in: 18-month-old (AL-18), 28-month-old (AL-28), and 32-month-old (AL-32) groups, and from CR-treated (CR) 28-month-old (CR-28) and 32-month-old (CR-32) counterparts to assay the effect of CR on several mitochondrial markers. The age-related decreases in citrate synthase activity, in TFAM, MFN2, and DRP1 protein amounts and in the mtDNA content in the AL-28 group were prevented in CR-28 counterparts. Accordingly, CR reduced oxidative mtDNA damage assessed through the incidence of oxidized purines at specific mtDNA regions in CR-28 animals. These findings support the anti-aging effect of CR up to 28 months. Conversely, the protein amounts of LonP1, Cyt c, OGG1, and APE1 and the 4.8 Kb mtDNA deletion content were not affected in CR-28 rats. The absence of significant differences between the AL-32 values and the CR-32 counterparts suggests an age-related boundary of CR efficacy at this age. However, this only partially curtails the CR benefits in counteracting the generalized aging decline and the related mitochondrial involvement.     

基于神经网络算法的井下裂缝诊断与堵漏技术

在钻井过程中，常常钻遇不同宽度的井下地层裂缝。钻遇裂缝时容易发生钻井液漏失现象，甚至发生钻井液失返现象，严重影响了安全、高效钻井。目前裂缝封堵的方法常存在封堵成功率不高、堵漏承压能力低的问题，其中一个重要的原因是对井下地层的裂缝宽度等特征认识不清。基于地层裂缝产生的岩石力学机理，确定影响裂缝宽度关键的6个力学和工程因素，并利用神经网络计算的非线性、大数据特点建立了井下地层裂缝宽度的分析模型，模型包含输入层、输出层和3个隐藏层。通过该模型诊断井下裂缝宽度，提高了计算精度，平均误差仅为2.09%，最大误差为5.88%，解决钻井现场仅凭经验判断裂缝误差较大和依靠成像测井成本较高的问题。同时根据神经网络模型诊断得到的裂缝宽度优化堵漏材料的粒径配比，提高了裂缝内的架桥封堵强度和架桥的稳定性，封堵层的承压能力达到12.8 MPa，反向承压能力达到4.5 MPa。现场堵漏试验最高憋压10 MPa，经过封堵作业后大排量循环不漏，达到了裂缝性地层高效堵漏的目的，堵漏一次成功。      

Application of differential evolution for cascaded multilevel VSI with harmonics elimination PWM switching

Harmonic elimination pulse width modulation (HEPWM) method has been widely applied to multilevel voltage source inverter (MVSI) to remove low frequency harmonics from its output voltage. However, the computation of the HEPWM switching angles for MVSI is very challenging due to several constraints, namely angle sequencing, very tight angular spacing and the numerous possibilities of angles distribution ratio. Realizing the potential of Differential Evolution (DE) to handle complex problems, this work proposes its application to solve the HEPWM problem for cascaded MVSI. Its emphasis is on improving the availability of HEPWM for higher output voltage by extending the maximum range of modulation index (M). It also removes the discontinuities in the switching angles and reduces the number of distribution ratio required to obtain the required solution. Compared to the most advanced (similar) work, i.e., 7-level MVSI with seventeen switching angles, DE covers a wider range of M; the maximum achievable M is 2.80. Furthermore, it exhibits very low second order distortion factor (DF₂): for the worst case, the value of DF₂ is 0.0014%. To verify the viability of the proposed algorithm, simulation is carried out and hardware prototype is constructed. Both results show very good agreement with the theoretical prediction.     

M1080B无心外圆磨床改进

M1080B无心外圆磨床砂轮及导轮宽度较窄,磨削轴承套圈外圆时,磨削效率低,托板架调整较为繁琐、皮带打滑、磨损较为严重。针对上述问题,提出了相应的解决办法,提高了磨削效率。     

AT89C2051芯片在车载产品中的应用

本文论述了采用单片机技术研制成功的泊车用超声波测距仪的基本原理,用AT89C2051作为控制器的实现方案,以及采用软件校正的方法,提高了测量精度和整机的可靠性.     

基于调谐频率与分频处理的高分辨率反演技术

讨论了基于调谐频率与分频处理的高分辨率反演技术，给出了方法的基本原理，即利用地震数据的调谐频率和分频处理分析，半定量地确定储层厚度，并将其作为地震反演的约束条件。方法的实现过程是：通过层状地质模型的地震正演模拟，建立不同主频子波时地层厚度与调谐频率的关系；利用地震资料时-频分析技术，实现地震资料分频处理；利用合成地震记录标定地震子波主频；利用分频处理结果，借助于调谐频率分析与调谐振幅分析，给出地震反演约束条件；利用反演算法消除地震子波影响，提高和拓展地震资料的频带范围，实现储层的高分辨率成像。普光地区的应用实例表明，调谐频率与分频处理的高分辨率反演方法无需对储层空间变化规律进行假设，适用于强非均质碳酸盐岩储层的高分辨率成像。     

混凝土小型砌块建筑裂缝宽度的标准设置研究

通过对混凝土小型空心砌块单片墙进行试验 ,了解不同构造措施墙体的初始裂缝在墙体上出现的部位、形态、发展规律以及墙体最终破坏形态 ,并通过试验明确墙体在不同受力状态下的裂缝形态 ,最后根据试验结果初步提出混凝土小型空心砌块建筑的裂缝宽度控制标准     

Determination of the width of the transition layer between the crystalline and amorphous zones in nylon 1010 by SAXS

The width of the transition layer between the crystalline and amorphous zones in nylon 1010 was determined by SAXS with point collimation and long-slit collimation, respectively. The width of the transition layer, E, was found to be 1.7 nm. The results show that the width of the transition layer is independent of crystallinity.     

泥沙输移矢量式及其应用

本文基于Parker等人泥沙输移矢量式的分析和研究，增加了上举力的作用，并考虑了泥沙颗粒受力非平衡条件，采用牛顿力学的矢量分析法，推导了泥沙输移的通用矢量式。经对公式简化，且用Ikeda和笔者试验资料的验证，证明计算值与试验值吻合较好。     

强度调制／直接检测（IM／DD）光纤通信系统色散限制的研究

通过引入归一化脉宽Ｐ和相对脉冲展宽因子ｑ，提出了一个适用于ＩＭ／ＤＤ系统中各种眼图恶化量ｘ和归一化脉宽Ｐ的色散限制改进公式。通过与其它理论的比较、计算机仿真和对实验数据的分析，验证了该公式的正确性，并给出了其它结果的限制条件。研究表明：当眼图恶化量ｘ为１ｄＢ左右时，对２．５Ｇｂ／；外调制信号（α＝０）而言，非色散位移光纤ＩＭ／ＤＤ系统的色散因子γ约等于０．７９，所对应的系统再生中继距离约为９００余公里。