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排序方式: 共有1044条查询结果,搜索用时 15 毫秒
1.
Gennaro Lettieri Rosaria Notariale Alessia Ambrosino Alfredo Di Bonito Antonella Giarra Marco Trifuoggi Caterina Manna Marina Piscopo 《International journal of molecular sciences》2021,22(4)
Mercury (Hg) is an environmental pollutant that impacts human and ecosystem health. In our previous works, we reported alterations in the properties of Mytilus galloprovincialis protamine-like (PL) proteins after 24 h of exposure to subtoxic doses of toxic metals such as copper and cadmium. The present work aims to assess the effects of 24 h of exposure to 1, 10, and 100 pM HgCl2 on spermatozoa and PL proteins of Mytilus galloprovincialis. Inductively coupled plasma–mass spectrometry indicated accumulation of this metal in the gonads of exposed mussels. Further, RT-qPCR analyses showed altered expression levels of spermatozoa mt10 and hsp70 genes. In Mytilus galloprovincialis, PL proteins represent the major basic component of sperm chromatin. These proteins, following exposure of mussels to HgCl2, appeared, by SDS-PAGE, partly as aggregates and showed a decreased DNA-binding capacity that rendered them unable to prevent DNA damage, in the presence of CuCl2 and H2O2. These results demonstrate that even these doses of HgCl2 exposure could affect the properties of PL proteins and result in adverse effects on the reproductive system of this organism. These analyses could be useful in developing rapid and efficient chromatin-based genotoxicity assays for pollution biomonitoring programs. 相似文献
2.
Guglielmina Chimienti Anna Picca Flavio Fracasso Francesco Russo Antonella Orlando Giuseppe Riezzo Christiaan Leeuwenburgh Vito Pesce Angela Maria Serena Lezza 《International journal of molecular sciences》2021,22(4)
Calorie restriction (CR) is the most efficacious treatment to delay the onset of age-related changes such as mitochondrial dysfunction. However, the sensitivity of mitochondrial markers to CR and the age-related boundaries of CR efficacy are not fully elucidated. We used liver samples from ad libitum-fed (AL) rats divided in: 18-month-old (AL-18), 28-month-old (AL-28), and 32-month-old (AL-32) groups, and from CR-treated (CR) 28-month-old (CR-28) and 32-month-old (CR-32) counterparts to assay the effect of CR on several mitochondrial markers. The age-related decreases in citrate synthase activity, in TFAM, MFN2, and DRP1 protein amounts and in the mtDNA content in the AL-28 group were prevented in CR-28 counterparts. Accordingly, CR reduced oxidative mtDNA damage assessed through the incidence of oxidized purines at specific mtDNA regions in CR-28 animals. These findings support the anti-aging effect of CR up to 28 months. Conversely, the protein amounts of LonP1, Cyt c, OGG1, and APE1 and the 4.8 Kb mtDNA deletion content were not affected in CR-28 rats. The absence of significant differences between the AL-32 values and the CR-32 counterparts suggests an age-related boundary of CR efficacy at this age. However, this only partially curtails the CR benefits in counteracting the generalized aging decline and the related mitochondrial involvement. 相似文献
3.
Antonella Ilenia Alfano Prof. Elisabetta Buommino Prof. Maria Grazia Ferraro Prof. Carlo Irace Prof. Angela Zampella Prof. Heiko Lange Prof. Margherita Brindisi 《ChemMedChem》2021,16(24):3795-3809
The generation of peptidomimetic substructures for medicinal chemistry purposes requires effective and divergent synthetic methods. We present in this work an efficient flow process that allows quick modulation of reagents for Joullié-Ugi multicomponent reaction, using spiroindolenines as core motifs. This sterically hindered imine equivalent could successfully be diversified using various isocyanides and amino acids in generally good space-time yields. A telescoped flow process combining interrupted Fischer reaction for spiroindolenine synthesis and subsequent Joullié-Ugi-type modification resulted in product formation in very good overall yield in less than 2 hours compared to 48 hours required in batch mode. The developed protocol can be seen as a general tool for rapid and facile generation of peptidomimetic compounds. We also showcase preliminary biological assessments for the prepared compounds. 相似文献
4.
5.
Gloria Ravegnini Bruno Fosso Viola Di Saverio Giulia Sammarini Federica Zanotti Giulio Rossi Monica Ricci Federica DAmico Giorgia Valori Antonella Ioli Silvia Turroni Patrizia Brigidi Patrizia Hrelia Sabrina Angelini 《International journal of molecular sciences》2020,21(24)
Gastric cancer (GC) is the fifth most prevalent cancer worldwide and the third leading cause of global cancer mortality. With the advances of the omic studies, a heterogeneous GC landscape has been revealed, with significant molecular diversity. Given the multifaceted nature of GC, identification of different patient subsets with prognostic and/or predictive outcomes is a key aspect to allow tailoring of specific treatments. Recently, the involvement of the microbiota in gastric carcinogenesis has been described. To deepen this aspect, we compared microbiota composition in signet-ring cell carcinoma (SRCC) and adenocarcinoma (ADC), two distinct GC subtypes. To this purpose, 10 ADC and 10 SRCC and their paired non-tumor (PNT) counterparts were evaluated for microbiota composition through 16S rRNA analysis. Weighted and unweighted UniFrac and Bray–Curtis dissimilarity showed significant community-level separation between ADC and SRCC. Through the LEfSe (linear discriminant analysis coupled with effect size) tool, we identified potential microbial biomarkers associated with GC subtypes. In particular, SRCCs were significantly enriched in the phyla Fusobacteria, Bacteroidetes, Patescibacteria, whereas in the ADC type, Proteobacteria and Acidobacteria phyla were found. Overall, our data add new insights into GC heterogeneity and may contribute to deepening the GC classification. 相似文献
6.
Ana Laura CIMADOR Emeli Luciana GALANTE Lucila Ibel MUÑOZ Patricia Silvia ROMANO Antonella Denisse LOSINNO María Cristina VANRELL 《Biocell》2019,43(1):13-20
Trypanosoma rangeli and T. cruzi are both parasitic unicellular species that infect humans. Unlike T. cruzi,
the causative agent of Chagas disease, T. rangeli is an infective and non-pathogenic parasite for humans, but pathogenic
for vectors from the Rhodnius genus. Because both species can coexist in different hosts and overlap their infective
cycles but very little is known about the infection of T. rangeli in mammalian cells, we decided to characterize both the
development of this parasite in cell culture and the effect of therapeutic agents with potential trypanocidal action on
it. We found that T. rangeli exhibits a cycle of infection in Vero cells similar to that for T. cruzi and that the repurposed
drug, 17-AAG, and the natural extract Artemisia sp. essential oil produce a toxic effect on epimastigotes showing
a trypanocidal action from the fifth day of culture. Both treatments also affected the infection of trypomastigotes
and reduced the capacity of replication of amastigotes of T. rangeli. Since T. cruzi / T. rangeli coinfection cases have
been reported, the finding of drugs with potential activity against both species could be significant in the future.
Furthermore, studies of susceptibility of both species to drugs could also help to know the different mechanisms of
pathogenicity in humans displayed by T. cruzi that are absent in T. rangeli 相似文献
7.
Andrea Bedon Jean Paul Viricelle Mathilde Rieu Simone Mascotto Antonella Glisenti 《International Journal of Hydrogen Energy》2021,46(27):14735-14747
In this contribution brownmillerite-based nanocomposite cathode for Single-Chamber Solid Oxide Fuel Cells is developed. These cells can be very attractive especially for small and cheap devices because of the absence of seals. The efficiency of SC-SOFCs is strictly connected to the selectivity of anode and cathode, the bottleneck for this technology. The development of a cathode inert in fuel oxidation is particularly challenging. Our strategy is to start from a catalytically un-active support (CFA = Ca2FeAl0.95Mg0.05O5) and induce the formation of iron oxide based nanoparticles, expected to activate oxygen. Symmetric (CFA + FeOx/CGO/CFA + FeOx) and complete cells (CFA + FeOx/CGO/Ni-CGO) are studied in air and methane/oxygen 2:1 mixture. The Area Specific Resistance of CFA + FeOx is less than 1/3 that of CFA. The high selectivity allows to reach an efficiency of 25%; power still needs to be increased but we demonstrated the possibility to develop selective low cost electrodes. The effect of air, methane/oxygen exposure and the heat treatments were carefully investigated. 相似文献
8.
Francisco Edvan Bezerra Feitosa Antonella Lombardi Costa 《International Journal of Hydrogen Energy》2021,46(40):20799-20814
This paper presents a case study focusing on fourteen most used energy alternatives in Brazil, possible to feed large scale hydrogen production plants for the automotive sector. The evaluations are made using a Decision Making Support Method, MACBETH - Measuring Attractiveness by a Categorical Based Evaluation Technique, with the computational code M-MACBETH 3.2.0, using criteria that include economic and financial, technological, environmental and social aspects. The selected criteria that were used in the assessment, for each of the energy alternatives are capital to be invested in a plant, leveled cost of electric energy produced, CO2 emissions, mortality rate due to the technology use and energy efficiency of technology. The main results obtained showed that photovoltaics off grid electric energy is the most attractive alternative, followed by the photovoltaic on grid alternative, for an eventual automotive hydrogen program in Brazil. 相似文献
9.
10.
Nitsa Buaron Antonella Mangraviti Francesco Volpin Ann Liu Mariangela Pedone Eric Sankey Dina Aranovich Itay Adar Fausto J. Rodriguez Abraham Nyska Riki Goldbart Tamar Traitel Henry Brem Betty Tyler Joseph Kost 《Advanced functional materials》2021,31(44):2100643
Treating neuroinflammation-related injuries and disorders through manipulation of neuroinflammation functions is being heralded as a new therapeutic strategy. In this study, a novel pectic galactan (PG) polysaccharide based gene therapy approach is developed for targeting reactive gliosis in neuroinflammation. Galectin-3 (Gal-3) is a cell protein with a high affinity to β-galactoside sugars and is highly expressed in reactive gliosis. Since PG carries galactans, it can target reactive gliosis via specific carbohydrate interaction between galactan and Gal-3 on the cell membrane, and therefore can be utilized as a carrier for delivering genes to these cells. The carrier is synthesized by modifying quaternary ammonium groups on the PG. The resulting quaternized PG (QPG) is found to form complexes with plasmid DNA with a mean diameter of 100 nm and have the characteristics required for targeted gene therapy. The complexes efficiently condense large amounts of plasmid per particle and successfully bind to Gal-3. The in vivo study shows that the complexes are biocompatible and safe for administration and can selectively transfect reactive glial cells of an induced cortical lesion. The results confirm that this PG-based delivery system is a promising platform for targeting Gal-3 overexpressing neuroinflammation cells for treating neuroinflammation-related injuries and neurodegenerative diseases. 相似文献