嵌入式Web控制终端的物联网移动E-Laboratory平台

为了进一步提高大学校园设备资源的利用率,实现多种实验装置的网络化控制和用户终端移动化,基于物联网技术,将LAMP移植到采用ARM架构的微处理器上,通过搭建嵌入式Web控制终端,对Web服务平台进行了详细的设计,实现了多种实验装置的网络化控制;将单自由度机械臂系统这一实验装置与EWC_T连接,基于Android操作系统上开发了一个应用,设计并搭建了Mobile Experiment系统,实现了网络化实验室平台的用户终端的移动化;通过实验证明该系统能够可靠地完成对实验装置的远程控制,并具有低功耗、低成本和便携性好等优点。     

基于LAMP的高性能Web服务器的架构

文章提出了基于LAMP的高性能Web服务器的架构方案,采用了Apache日志、Webalizer日志分析、Cacti流量监控、入侵检测的方法,架构了一个完善的、稳定的、安全的、低廉的高性能Web服务器,满足了中小型企业的要求。     

在Ubuntu平台上部署基于Snort的入侵检测系统

Snort是一套免费的轻量级入侵检测系统,当今的很多入侵检测系统都以其为参照。虽然Snort软件体积非常小巧,但是其部署有一定的难度。为了以更直观的方式监测系统的运行状况,通常部署Snort系统时除了要安装Snort软件本身外,还需要大量的相关软件支持。文章介绍了在Ubuntu平台上架设snort系统的具体过程。系统以MysQL数据库存储入侵检测信息,用BASE工具显示检测结果。     

基于开源LAMP架构的Web打印技术

通过对现有B/S架构打印方法进行分析对比,针对它们的缺陷提出了一种基于开源LAMP架构的跨平台、精确、有效的Web打印新技术,并详细介绍了其原理,最后给出了具体的实现方法.     

Real time loop-mediated isothermal amplification using a portable fluorescence scanner for rapid and simple detection of Vibrio parahaemolyticus

We evaluated the usefulness of the loop-mediated isothermal amplification (LAMP) using a portable ESE Quant tube scanner as a rapid and simple method for the detection of Vibrio parahaemolyticus, an important pathogen causing seafood-borne gastroenteritis. The real time LAMP (RT-LAMP) assay using a hemolysin gene (tlh/ldh)-specific primers was verified using V. parahaemolyticus strains (n = 91) from different countries and other non-target strains to check the utility of this method. Both the sensitivity and specificity of the RT-LAMP using 3 pairs of tlh/ldh-specific primers developed in this study were excellent (100%). The detection limit of the RT-LAMP was as low as 7 Colony forming unit per reaction and detection time was only 20 min. Comparative evaluation of the target bacterial strains with the RT-LAMP using ESE Quant tube scanner, API 20E system and conventional RT-PCR method revealed that the RT-LAMP assay developed in this study is simpler and more rapid than the latter two methods. Therefore, the RT-LAMP method using the easily portable ESE Quant tube scanner can be considered as an effective tool for the rapid screening of V. parahaemolyticus strains in environmental and clinical samples, especially, in remote areas of developing countries during epidemic periods.     

环介导恒温扩增法鉴定牛羊肉中的搀杂肉

建立了一个鉴定牛羊肉中搀杂杂动物肉的环介导恒温扩增检测方法。确定了一套可在牛羊肉中特异并灵敏地检测出搀杂肉成分的引物对,以动物细胞色素b基因组为模板可在恒温63℃恒温特异性扩增出猪等基因片段而无其他扩增片段影响。可检测牛肉中0.01%～2%的猪肉成分。经与PCR方法比对,结果表明,该方法可有效用于实际生鲜肉或加工肉制品样本的鉴定。     

食品中大肠杆菌0157: H7的几种检测方法的比较

目的 评估平板分离培养法、免疫磁珠分离(IMS)法、VIDAS全自动酶标免疫测试系统、BAX全自动病原菌检测系统及环介导等温扩增(LAMP)技术在食品中检验肠出血型大肠埃希菌0157:H7的特异性、敏感性.方法 使用平板分离培养法、免疫磁珠分离法、VIDAS法、BAX法及LAMP法对人工制备的染菌猪肉样本进行检测,并对这几种方法进行比较.结果 BAX法和LAMP法的检出率最高,分别是89.1％和85.9％,免疫磁珠法和VIDAS法检出率次之,分别是75.0％和78.1％,传统分离培养法为43.8％.结论 BAX法和LAMP法具有快速、高效、特异性好、敏感性高的特点,可快速筛选食品中可能存在的肠出血型大肠埃希菌0157∶H7.     

Loop‐Mediated Isothermal Amplification (LAMP): A Rapid and Sensitive Tool for Quality Assessment of Meat Products

Loop‐mediated isothermal amplification (LAMP) is a novel method that amplifies target nucleic acids under isothermal conditions. It is a rapid, specific, and sensitive method, which does not require costly thermal cyclers for the detection of nucleic acids. Thus, it is suitable for on‐site detection assays under low‐resource settings. It can also be integrated on compact lab‐on‐a‐chip devices for the development of micro‐total analysis systems. This review discusses LAMP‐based methods, as well as LAMP‐based centrifugal, microfluidic, and other fluid‐handling devices, which have been developed for the assessment of meat quality parameters that are related to the presence or absence of nucleic acids, for example, animal species identification and microbiological quality. Advances in improving the rapidity, specificity, and sensitivity of LAMP techniques for the assessment of these meat quality parameters are also discussed in this review.     

基于环介导等温扩增技术的产呕吐毒素 蜡样芽胞杆菌特异性检测

本研究旨在建立食品中产呕吐毒素蜡样芽胞杆菌的快速检测方法。基于产呕吐毒素蜡样芽胞杆菌合成酶基因cesB靶基因,设计4条特异性引物(2条内引物、2条外引物),建立环介导等温扩增技术(LAMP)。然后采用2株产呕吐毒素蜡样芽胞杆菌、19株蜡样芽胞杆菌和41株非蜡样芽胞杆菌验证了该LAMP具有很好的特异性。LAMP检测的灵敏度在DNA水平上达到1.49 pg/μL,纯菌的灵敏度为5×10~3 cfu/mL。人工污染米饭样品,当起始污染量为2 cfu/g时,37℃增菌培养6 h,用试剂盒法和煮沸法提取的DNA,都可以检出产呕吐毒素蜡样芽胞杆菌。采用此LAMP方法进行了72份实际样品的检测,检出2份产呕吐毒素蜡样芽胞杆菌阳性样品,与传统方法一致。因此,本研究建立的LAMP检测方法可应用于食品中产呕吐毒素蜡样芽胞杆菌的快速特异检测。     

Effect of Penicillium nordicum contamination rates on ochratoxin A accumulation in dry-cured salami

Fungal starter, such as Penicillium nalgiovense, are commonly used to inoculate sausages before seasoning process. However, Penicillium nordicum, a well-known ochratoxin A (OTA) producer frequently isolated from seasoning rooms, could colonize the casing surface during the early stage of production. The relationship between OTA accumulation and simultaneous inoculation of P. nalgiovense and P. nordicum at different rates was evaluated. After 14 days of seasoning, the persistence of P. nordicum was assessed by LAMP assay revealing its capability to colonize and grow on salami surface at all the contamination rates. At the end of seasoning, OTA was accumulated both in mycelium and dry-cured meat when P. nordicum contamination rate ranged from 25% to 100% of inoculum, while no OTA was detected in dry-cured meat at 2.5% and 0.25%. Results demonstrated that contamination of fungal starter by P. nordicum could represent a serious concern during salami production and therefore represents an important critical point to be monitored.