嗜酸乳杆菌和乳酸链球菌混合培养及产物分析

嗜酸乳杆乳菌(LA)和酸链球菌(SL)在33℃培养48 h的发酵液中抑菌(金黄色葡萄球菌ATCC25923)效果最好;2菌混培20h后生物量和体系总产酸量远大于各自纯培养,乳酸含量一直上升,到24 h达23.175 mg/L,乙酸含量一直稳定在12 mg/L左右,变化不大,乳酸与抑菌关系密切。LA和SL两菌混培接种比例的抑菌优先顺序为(1:1)、(2:1)、(1:2)。     

共培养微藻Monoraphidium sp. eh1和Monoraphidium sp. eh3提高油脂产率和沉降率

为了提高微藻油脂产率,降低其采收成本,对比了微藻Monoraphidium sp. eh1和Monoraphidium sp. eh3单独培养和共培养方式的影响,同时对于培养结束后提升微藻沉降率的方法进行了研究。结果表明:微藻Monoraphidium sp. eh1和Monoraphidium sp. eh3单独培养的油脂产率分别为17. 45 mg/(L·d)和15. 35 mg/(L·d),而共培养油脂产率可达到38. 82 mg/(L·d);通过共培养后调节藻液pH至7. 0的方法,30 min沉降率可达到95. 38%,与添加絮凝剂的沉降效果相当,相较于单独培养的eh1、eh3的沉降率10. 01%和53. 60%有显著提升。共培养技术结合调节pH的方式,可以为提高微藻油脂产率和降低采收成本提供一种具有可行性的解决方案。     

Fundamental and Clinical Applications of Materials Based on Cancer-Associated Fibroblasts in Cancers

Cancer stromal cells play a role in promoting tumor relapse and therapeutic resistance. Therefore, the current treatment paradigms for cancers are usually insufficient to eradicate cancer cells, and anti-cancer therapeutic strategies targeting stromal cells have been developed. Cancer-associated fibroblasts (CAFs) are perpetually activated fibroblasts in the tumor stroma. CAFs are the most abundant and highly heterogeneous stromal cells, and they are critically involved in cancer occurrence and progression. These effects are due to their various roles in the remodeling of the extracellular matrix, maintenance of cancer stemness, modulation of tumor metabolism, and promotion of therapy resistance. Recently, biomaterials and nanomaterials based on CAFs have been increasingly developed to perform gene or protein expression analysis, three-dimensional (3D) co-cultivation, and targeted drug delivery in cancer treatment. In this review, we systematically summarize the current research to fully understand the relevant materials and their functional diversity in CAFs, and we highlight the potential clinical applications of CAFs-oriented biomaterials and nanomaterials in anti-cancer therapy.     

高职校企合作“双融共育”教学体系的构建

校企合作是高职教育体系的重要内容,也是高职院校生存和发展的客观要求和必然趋势.针对当前高职教育校企合作发展的现实困境,探索构建“双融共育”的校企合作教学体系,深入分析校企合作的内涵和机制并提出相应的实施思路,以促进我国高职教育校企合作的健康有序发展.     

建筑业卓越管理工程师校企协同培养平台研究

根据"卓越计划"坚持校企结合的导向原则,结合建筑业的行业特点,构建基于"卓越管理工程师"培养的工程管理校企协同培养平台,界定该平台的概念和功能,分析高校、企业、政府及相关中介机构在平台中的地位与作用,探讨培养平台的构成系统、运行模式以及平台运行效果的评价体系。     

校企双主体携手,共育新时代工匠

随着我国汽车产业的迅速发展,市场对汽车维修人才的要求越来越高。职业院校如何培养学生较强的岗位适应能力、更高的职业技能和职业素养是满足汽车企业对高素质技术技能人才需求的关键环节。汽车检测与维修技术专业实施现代学徒制人才培养模式,在校企协同育人、共同制定人才培养方案和课程标准、共建双导师团队等方面进行了探索和总结。     

共培养微藻Monoraphidium sp. FXY-10 与Chlorella sp. U4341提高油脂产率与沉降率

采用共培养技术,在异养条件下培养微藻Chlorella sp.U4341和Monoraphidium sp.FXY-10,提高两株微藻共培养的油脂产率;通过共培养方式促进微藻细胞的自絮凝,加速微藻细胞的沉降,以期降低微藻生物量的采收成本。结果表明:微藻Chlorella sp.U4341与Monoraphidium sp.FXY-10单独培养的油脂产率分别为272.07、268.54 mg/(L·d),而两株微藻共培养的油脂产率提高到315.60 mg/(L·d);两株微藻共培养后,自然沉降5 h,微藻细胞沉降率高于90%,远高于单独培养条件下的沉降率。因此,微藻的共培养技术有望成为解决微藻油脂产率低、采收成本高两大瓶颈问题的解决方案。