红外光控器在蜜丸包装机上的应用

中药大蜜丸及所用包装材料软、粘、轻、小、光,较难实现机械化自动包装。通常的监控方法不适合蜜丸的自动包装。采用红外线光电控制器,解决了自动包装蜜丸时出现的瞬间无纸、无丸等自动控制。     

基于改进HBA算法的生鲜闭环供应链网络鲁棒优化设计

针对生鲜闭环供应链网络设计问题,建立了一种基于生鲜闭环供应链网络的鲁棒优化模型,以解决供应链网络中的不确定性问题。首先,针对涵盖五个节点的生鲜供应链网络结构建立了多周期、多产品,以最小化成本、最小环境影响为目标的混合整数规划模型,采用模糊折中规划与区间数据鲁棒优化方法进行处理;其次,在原有蜜獾算法的基础上引入差分进化原则,增强算法的全局搜索能力与收敛速度;最后,通过MATLAB数值分析与仿真实例表明,所提鲁棒优化模型与蜜獾算法在求解生鲜闭环供应链网络设计问题中具有明显优势。     

八种蜂蜜挥发性成分分析

用同时蒸馏萃取方法分别收集了洋槐蜜、霍香蜜、紫云英蜜、瑞苓草蜜、苜蓿蜜、油菜蜜、枸杞蜜和龙眼蜜的挥发性成分,所得到的8种蜂蜜挥发性成分的二氯甲烷浓缩液,用气相色谱-质谱(GC-MS)法分离并分析鉴定了其组成及质量分数,分别鉴定出19、32、17、20、22、64、21、27个化合物,各占其总峰面积的93.35%、76.93%、38.58%、93.18%、93.92%、85.66%、46.37%、75.01%。     

Mandibular gland components of european and africanized honey bee queens (Apis mellifera L.)

The composition of the five-component honey bee queen mandibular gland pheromone (QMP) of mated European honey bee queens was compared to those of virgin and drone-laying (i.e., laying only haploid unfertilized eggs that develop into males), European queens and Africanized mated queens. QMP of mated European queens showed significantly greater quantities of individual components than all queen types compared, except for a significantly greater quantity of 9-hydroxy-(E)-2-decenoic acid (9-HDA) found in Africanized queens. Glands of European drone-laying queens contained quantities intermediate between virgin and mated queens, reflecting their intermediate reproductive state and age. QMP ontogeny shifts from a high proportion of 9-keto-(E)-2-decenoic acid (ODA) in young unmated queens to roughly equal proportions of ODA and 9-HDA in mated queens. A biosynthetic shift occurs after mating that results in a greater proportion of 9-HDA, methylp-hydroxybenzoate (HOB), and 4-hydroxy-3-methoxyphenylethanol (HVA) production, accompanied by a decreased proportion of ODA. Africanized QMP proportions of ODA and 9-HDA were significantly different from European queens. A quantitative definition of a queen equivalent of QMP is proposed for the various queen types, and a standard queen equivalent for mated European honeybee queen mandibular gland pheromone is adopted as 200µg ODA, 80µg 9-HDA, 20µg HOB, and 2 µg HVA.     

空间机器人随机故障容错规划的蜜蜂算法

本文为解决复杂的随机规划问题设计了一种基于随机模拟的混沌量子蜜蜂算法,证明了该算法的收敛 性,并分析了算法的收敛速度．分析6 自由度空间机器人系统的不确定性,采用基于微分变换法进行误差分析,建 立了随机数学规划模型．为涉及故障前后运动学与动力学约束限制的容错轨迹规划,以加权最小驱动力矩为优化性 能指标,采用混沌量子蜜蜂算法求解全部工作时间中机械臂故障前后的最优轨迹．通过降低异常关节的运动速度来 降低故障关节力矩,保证机械臂在发生故障后具有较高的操作能力．案例研究验证了该算法的有效性、稳定性及准 确性．     

Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods

A gold immunochromatographic sensor (GICS) was developed for the rapid detection of 26 sulfonamides in honey samples.The sensor was based on a group-specific monoclonal antibody (mAb) that can recognize all 26 sulfonamides.Three haptens (hapten 1 with a thiazole ring,hapten 2 with a benzene ring,and hapten 3 with a straight carbon chain) were used for antigen preparation.With hybridoma technology,a group-specific mAb was screened with a 50％ maximal inhibitory concentration (IC50) against sulfathizole (STZ) and the other 25 analogues ranging from 0.08 to 90.18 ng/mL.Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip.A labeled antibody concentration of 0.1 μg/mL and a coating antigen concentration of 0.2 μg/mL in the test line were chosen for strip preparation.Under optimized conditions,the visual limits of detection (vLOD) for the concentrations of STZ,sulfamethoxazole,sulfamethizole,sulfadiazine,sulfamerazine,sulfadimethoxine,sulfamonomethoxine,sulfameter,sulfamethoxypyridazine,and sulfachloropyridazine were 5,0.25,0.25,10,5,10,25,2.5,5,0.25,and 10 μg/kg,respectively.Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides.Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography.The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples.     

Floral nectar phenolics as biochemical markers for the botanical origin of heather honey

In order to find out biochemical markers for the botanical origin of heather (Erica) honey, the phenolic metabolites present in heather floral nectar, collected from the honey-stomach of bees gathering nectar from these flowers, were analysed. The flavonoid fraction of nectar contained four main flavonoids. Two of them were quercetin and kaempferol 3-rhamnosides, and the other two were tentatively identified as myricetin 3-methyl ether and isorhamnetin 3-rhamnosides. Since the natural glycosides are hydrolysed by bee enzymes to render the corresponding aglycones, which are the metabolites detected in honey, acid hydrolysis of the nectar glycosides was achieved. The aglycones quercetin, myricetin 3-methyl ether, kaempferol and isorhamnetin were identified, as well as the gallic acid derivative ellagic acid. The analysis of Portuguese heather honey samples showed that ellagic acid was present in all the samples in significant amounts ranging between 100 g and 600 g per 100 g honey. The other nectar-derived flavonoids were also present, although some of them in very variable amounts. Ellagic acid and myricetin 3-methyl ether, which have not been detected in any of the monofloral honey samples investigated so far, with the only exception being a French honey sample of the botanically relatedCalluna (Ericaceae) which also contained ellagic acid, seem to be the most useful potential markers for the floral origin of heather honey. However, more detailed and extensive investigations are needed to prove the utility of these markers.     

凝固型蜂蜜酸奶的研制

凝固型蜂蜜酸奶是一种很有发展前景的高级饮品,以牛奶、蜂蜜为主要原料,有可口的酸甜味和特有的清香味,具有蜂蜜和牛奶双重的营养保健作用.同时蜂蜜酸奶的开发,也为蜂蜜的开发利用提供了一条新途径.该论文论述了新型凝固型蜂蜜酸奶的研制过程,主要从蜂蜜的用量、发酵时间、稳定剂用量和培养温度等各方面进行探索,确定了较佳的工艺参数,并进行了产品质量检测.结果表明:在蜂蜜添加量为6%,发酵剂为9%,琼脂为0.10%,于40～43℃条件下,产品风味最好,具有蜂蜜特有的清香味,酸甜宜口,受到消费者的欢迎.     

Real-time quality authentication of honey using atmospheric pressure chemical ionisation mass spectrometry (APCI-MS)

The aim of this study was to use gas chromatography-mass spectrometry (GC-MS) and APCI-MS techniques to detect adulteration in honey. The key volatile compounds in the headspace of the adulterated honey were marked by GC-MS and their representative fragment ions were utilised in scanning honey samples using the real-time APCI-MS system. The PLS models validated using independent data sets resulted in coefficient of the determination () of 0.97 and 0.96 and root mean square error in prediction (RMSEP) of 2.62 and 2.45 for the GC-MS and APCI-MS data sets respectively. The most efficient volatiles from GC-MS analysis and their corresponding fragment ions m/z from APCI-MS data analysis were then identified and used to develop new PLS models to predict the level of adulteration. The best PLS model gave of 0.95 and RMEP of 2.60% in the independent validation set indicating that the model was very accurate in predicting the level of adulteration.     

Geographical characterisation of honeys according to their mineral content and antioxidant activity using a chemometric approach

In this article, discrimination models are presented, relating the origin of honey samples to several variables, being the concentrations of different cations and anions in the honey samples measured by ion chromatography, and parameters that measure/reflect the antioxidant activity of the honey samples. The unsupervised method, principal component analysis, and supervised discrimination methods, such as linear and quadratic discriminant analysis, and classification and regression trees (CART), were applied to evaluate the existence of data patterns and the relationship between geographical origin and the measured parameters. The model with the best predictive ability (%CCR_TEST = 66.67%), the best overall % specificity (80%) and the best overall % sensitivity (67%) was found to be CART. It was proven that the mineral content and parameters analysed can provide enough information for the geographical characterisation and discrimination of honey.