Real-Time Duplex Applications of Loop-Mediated AMPlification (LAMP) by Assimilating Probes

Isothermal nucleic-acid amplification methods such as Loop-Mediated isothermal AMPlification (LAMP) are increasingly appealing alternatives to PCR for use in portable diagnostic system due to the low cost, weight, and power requirements of the instrumentation. As such, interest in developing new probes and other functionality based on the LAMP reaction has been intense. Here, we report on the development of duplexed LAMP assays for pathogen detection using spectrally unique Assimilating Probes. As proof of principle, we used a reaction for Salmonella enterica as a model coupled with a reaction for λ-phage DNA as an internal control, as well as a duplexed assay to sub-type specific quarantine strains of the bacterial wilt pathogen Ralstonia solanacearum. Detection limits for bacterial DNA analyzed in individual reactions was less than 100 genomic equivalents in all cases, and increased by one to two orders of magnitude when reactions were coupled in duplexed formats. Even so, due to the more robust activity of newly available strand-displacing polymerases, the duplexed assays reported here were more powerful than analogous individual reactions reported only a few years ago, and represent a significant advance for incorporation of internal controls to validate assay results in the field.     

分布式光纤喇曼放大技术及其在WDM系统中的应用

介绍了分布式光纤喇曼放大技术的原理、特点和应用 ,并从受此影响的 WDM传输系统的信噪比、噪声指数和品质因数三个方面讨论了光纤喇曼放大技术在提升光纤通信系统性能方面的重要作用。     

Current and emerging molecular diagnostic technologies applicable to bacterial food safety

There is an increasing need for rapid test methods to certify the quality and safety of food products. Current tests applied for the microbiological assessment of food products are based on standard approved culture-based isolation methods and can take several days to yield results. Nucleic acid diagnostic (NAD) tests for the identification of bacterial foodborne pathogens employing in vitro amplification technologies are capable of sensitive and specific detection of single or multiple pathogens in foods in a shorter timeframe than traditional methods. New developments in molecular biosensors have the potential to provide at-line bioanalysis, whereas microarray-based technologies may in the future be the NAD platforms of choice for multiple pathogen detection and identification. This article reviews current and emerging NAD platforms for foodborne bacterial pathogens that have the potential to impact food safety.     

光纤喇曼放大器的功率转换效率分析

在定义光纤喇曼放大器(FRA)功率转换效率(PCE)的基础上,考察了不同工作条件下光纤喇曼放大器的功率转换效率,分析PCE与光纤喇曼放大器的输入信号功率、输入泵浦功率和光纤长度的关系,比较了分布式与集总式FRA的功率转换效率,给出提高FRA功率转换效率的途径。     

基于DDS改进BT-4型扫频仪

基于DDS技术,改造BT 4型扫频仪中的扫频信号发生器,使其具有高稳定度、高分辨率、高精度的特点。同时为了使扫频仪能够对被测电路的动态范围进行检测,使扫频仪得到更广泛的应用,运用了AGC控制技术,首先使电压稳定在10 V;然后通过控制D/A使输出电压按测试需要输出。硬件给出了具体电路参数,软件给出了相应软件流程,总结了其他波型产生的方法。     

A positive‐working photosensitive polyimide based on thermal cross‐linking and acidolytic cleavage

A novel positive‐working photosensitive polyimide (PSPI) based on a poly(hydroxyimide) (PHI), a crosslinking agent having vinyl ether groups, and a photoacid generator (PAG) was prepared. The PHI as a base resin of the three‐component PSPI was synthesized from 4,4′‐oxydiphthalic anhydride and 2,2′‐bis(3‐amino‐4‐hydroxyphenyl)hexafluoropropane through ring‐opening polymerization and subsequent thermal cyclization. 2,2′‐bis(4‐(2‐(vinyloxy)ethoxy)phenyl)propane (BPA‐DEVE) was used as a vinylether compound and diphenyliodonium 5‐hydroxynaphthalene‐1‐sulfonate was used as a PAG. The phenolic hydroxyl groups of the PHI and the vinyl ether groups of BPA‐DEVE are thermally crosslinked with acetal structures during prebake step, and the crosslinked PHI becomes completely insoluble in an aqueous basic solution. Upon exposure to UV light (365 nm) and subsequent postexposure bake (PEB), a strong acid generated from the PAG cleaves the crosslinked structures, and the exposed area is effectively solubilized in the alkaline developer. The dissolution behavior of the PSPI containing each 11.5 wt % of BPA‐DEVE and of the PAG was studied after UV exposure (365 nm) and PEB. It was found that the difference in dissolution rates between exposed and unexposed areas was enough to get high resolution. A fine positive pattern with a resolution of 5 μm in a 3.7‐μm‐thick film was obtained from the three‐component PSPI. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

A novel data-driven approach to support decision-making during production scale-up of assembly systems

In today's manufacturing settings, a sudden increase in the customer demand may enforce manufacturers to alter their manufacturing systems either by adding new resources or changing the layout within a restricted time frame. Without an appropriate strategy to handle this transition to higher volume, manufacturers risk losing their market competitiveness. The subjective experience-based ad-hoc procedures existing in the industrial domain are insufficient to support the transition to a higher volume, thereby necessitating a new approach where the scale-up can be realised in a timely, systematic manner. This research study aims to fulfill this gap by proposing a novel Data-Driven Scale-up Model, known as DDSM, that builds upon kinematic and Discrete-Event Simulation (DES) models. These models are further enhanced by historical production data and knowledge representation techniques. The DDSM approach identifies the near-optimal production system configurations that meet the new customer demand using an iterative design process across two distinct levels, namely the workstation and system levels. At the workstation level, a set of potential workstation configurations are identified by utilising the knowledge mapping between product, process, resource and resource attribute domains. Workstation design data of selected configurations are streamlined into a common data model that is accessed at the system level where DES software and a multi-objective Genetic Algorithm (GA) are used to support decision-making activities by identifying potential system configurations that provide optimum scale-up Key Performance Indicators (KPIs). For the optimisation study, two conflicting objectives: scale-up cost and production throughput are considered. The approach is employed in a battery module assembly pilot line that requires structural modifications to meet the surge in the demand of electric vehicle powertrains. The pilot line is located at the Warwick Manufacturing Group, University of Warwick, where the production data is captured to initiate and validate the workstation models. Conclusively, it is ascertained by experts that the approach is found useful to support the selection of suitable system configuration and design with significant savings in time, cost and effort.     

等温核酸放大技术在重金属检测方面的应用

等温核酸放大技术是一种可以实现重金属污染物痕量分析的高灵敏性检测方法,在分析检测中有着广泛且重要的作用。本文综述了几种不同的等温核酸放大技术及其在重金属检测方面的应用,阐述分析了各方法的检测原理,为之后建立更加灵敏、快速、准确的检测方法提供了参考。虽然等温核酸放大技术目前主要停留在实验室阶段,并且检测目标有限,但是由于该技术的高灵敏性、特异性以及与其他学科紧密的联系性,具有潜在的应用前景。     

248nm深紫外光刻胶

本文从化学增幅技术的产生，深紫外248nm胶主体树脂及PAG发展历程、溶解抑制剂、存在的工艺问题及解决途径多个方面综述了深紫外248nm胶的发展与进步．