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排序方式: 共有110条查询结果,搜索用时 460 毫秒
1.
大小样品在液氮中的淬冷沸腾换热比较研究 总被引:1,自引:1,他引:0
分析了大小样品在液氮中的淬冷沸腾换热特性。大小样品的淬冷沸腾换热有着明显的差别,小样品的热流密度比大样品的明显高得多。小样品的淬冷沸腾有一起始段,其温降高达60K。从实验结果还可以看到,小样品的Leidenfrost点和临界热流点对应的壁面过热度明显比大样品的高。最后还分析了样品直径对临界热流密度的影响。 相似文献
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Oleksandr Gryshkov Vitalii Mutsenko Dmytro Tarusin Diaa Khayyat Ortwin Naujok Ekaterina Riabchenko Yuliia Nemirovska Arseny Danilov Alexander Y. Petrenko Birgit Glasmacher 《International journal of molecular sciences》2021,22(6)
Alginate as a versatile naturally occurring biomaterial has found widespread use in the biomedical field due to its unique features such as biocompatibility and biodegradability. The ability of its semipermeable hydrogels to provide a favourable microenvironment for clinically relevant cells made alginate encapsulation a leading technology for immunoisolation, 3D culture, cryopreservation as well as cell and drug delivery. The aim of this work is the evaluation of structural properties and swelling behaviour of the core-shell capsules for the encapsulation of multipotent stromal cells (MSCs), their 3D culture and cryopreservation using slow freezing. The cells were encapsulated in core-shell capsules using coaxial electrospraying, cultured for 35 days and cryopreserved. Cell viability, metabolic activity and cell–cell interactions were analysed. Cryopreservation of MSCs-laden core-shell capsules was performed according to parameters pre-selected on cell-free capsules. The results suggest that core-shell capsules produced from the low viscosity high-G alginate are superior to high-M ones in terms of stability during in vitro culture, as well as to solid beads in terms of promoting formation of viable self-assembled cellular structures and maintenance of MSCs functionality on a long-term basis. The application of 0.3 M sucrose demonstrated a beneficial effect on the integrity of capsules and viability of formed 3D cell assemblies, as compared to 10% dimethyl sulfoxide (DMSO) alone. The proposed workflow from the preparation of core-shell capsules with self-assembled cellular structures to the cryopreservation appears to be a promising strategy for their off-the-shelf availability. 相似文献
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Sheng-Hsiang Li Yuh-Ming Hwu Chung-Hao Lu Hsiao-Ho Chang Cheng-En Hsieh Robert Kuo-Kuang Lee 《International journal of molecular sciences》2016,17(8)
This study was conducted to investigate the effect of the vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2) on revascularization, survival, and oocyte quality of cryopreserved, subcutaneously-transplanted mouse ovarian tissue. Autologous subcutaneous transplantation of vitrified-thawed mouse ovarian tissues treated with (experimental group) or without (control group) VEGF and FGF2 was performed. After transplantation to the inguinal region for two or three weeks, graft survival, angiogenesis, follicle development, and oocyte quality were examined after gonadotropin administration. VEGF coupled with FGF2 (VEGF/FGF2) promoted revascularization and significantly increased the survival rate of subcutaneously-transplanted cryopreserved ovarian tissues compared with untreated controls. The two growth factors did not show long-term effects on the ovarian grafts. In contrast to the untreated ovarian grafts, active folliculogenesis was revealed as the number of follicles at various stages and of mature oocytes in antral follicles after gonadotropin administration were remarkably higher in the VEGF/FGF2-treated groups. Although the fertilization rate was similar between the VEGF/FGF2 and control groups, the oocyte quality was much better in the VEGF/FGF2-treated grafts as demonstrated by the higher ratio of blastocyst development. Introducing angiogenic factors, such as VEGF and FGF2, may be a promising strategy to improve revascularization, survival, and oocyte quality of cryopreserved, subcutaneously-transplanted mouse ovarian tissue. 相似文献
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Effects of nanoparticles on the nucleation and devitrification temperatures of polyol cryoprotectant solutions 总被引:2,自引:0,他引:2
To investigate the efficiency of nanoparticles as nucleation agents in polyol cryoprotectant solutions at cryogenic temperatures,
the nucleation and devitrification temperatures of these solutions with different concentrations of diamond, gold, copper–nickel,
and silicon nanoparticles were measured through differential scanning calorimetry. The results show that ice growth in these
cryoprotectant solutions is prominently accelerated by nanoparticles and the devitrification temperatures of cryoprotectant
solutions are significantly lowered by nanoparticles. These findings can be used to improve the efficiency of cell cryopreservation
procedures and open numerous avenues of investigations regarding the application of nanoparticles in cryogenics. 相似文献
7.
Josephine H. Cox Guido Ferrari Robert T. Bailer Richard A. Koup 《Journal of The Association for Laboratory Automation》2004,9(1):16-23
Cell-mediated immune responses have been found to play a crucial role in the protection from, or amelioration of, many human diseases. There are many vaccine trials being conducted where the most important immune assessment consists of measuring T cell responses. The most readily accessible source of T cells is the peripheral blood. Thus, collection, processing, cryopreservation, storage, and manipulation of human peripheral blood mononuclear cells (PBMCs) are all key steps for assessment of vaccine and disease-induced immune responses. Automation of these procedures is not currently practiced widely. This article reviews current practices and explores the need for automation. 相似文献
8.
在微电脑控制的小样品升降温控制装置上,用透射电子显微镜研究了鼠肾组织在快速冷却后冰晶生长的一些特点,并结合传热分析方法和结晶动力学理论,定量地研究了肾细胞核内冰晶随位置的分布。 相似文献
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