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排序方式: 共有1430条查询结果,搜索用时 15 毫秒
51.
Tomasz Pdzinski Katarzyna Grzyb Konrad Skotnicki Piotr Filipiak Krzysztof Bobrowski Chryssostomos Chatgilialoglu Bronislaw Marciniak 《International journal of molecular sciences》2021,22(9)
Within the reactive oxygen species (ROS) generated by cellular metabolisms, hydroxyl radicals (HO•) play an important role, being the most aggressive towards biomolecules. The reactions of HO• with methionine residues (Met) in peptides and proteins have been intensively studied, but some fundamental aspects remain unsolved. In the present study we examined the biomimetic model made of Ac-Met-OMe, as the simplest model peptide backbone, and of HO• generated by ionizing radiation in aqueous solutions under anoxic conditions. We performed the identification and quantification of transient species by pulse radiolysis and of final products by LC-MS and high-resolution MS/MS after γ-radiolysis. By parallel photochemical experiments, using 3-carboxybenzophenone (CB) triplet with the model peptide, we compared the outcomes in terms of short-lived intermediates and stable product identification. The result is a detailed mechanistic scheme of Met oxidation by HO•, and by CB triplets allowed for assigning transient species to the pathways of products formation. 相似文献
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53.
Krzysztof Lukaszkowicz Leszek A. DobrzanskiGrzegorz Kokot Pawel Ostachowski 《Vacuum》2012,86(12):2082-2088
The paper presents the research results on the microstructure, mechanical and tribological properties of coatings deposited by cathodic arc evaporation methods on the X40CrMoV5-1 hot work tool steel substrates as well as the possibility to employ the finite element method for evaluation of stresses in the investigated coatings. The tests were carried out on CrAlSiN and CrN coatings. The coatings demonstrated a dense cross-sectional morphology as well as good adhesion to the substrate. The critical load LC2 lies within the range 54-55 N, depending on the coating type. The investigated coatings have the potential to increase the service life of an extrusion die by orders of magnitude as compared with uncoated steel. 相似文献
54.
Matthew A. A. Grant Bart?omiej Wac?aw Rosalind J. Allen Pietro Cicuta 《Journal of the Royal Society Interface》2014,11(97)
Mechanical forces are obviously important in the assembly of three-dimensional multicellular structures, but their detailed role is often unclear. We have used growing microcolonies of the bacterium Escherichia coli to investigate the role of mechanical forces in the transition from two-dimensional growth (on the interface between a hard surface and a soft agarose pad) to three-dimensional growth (invasion of the agarose). We measure the position within the colony where the invasion transition happens, the cell density within the colony and the colony size at the transition as functions of the concentration of the agarose. We use a phenomenological theory, combined with individual-based computer simulations, to show how mechanical forces acting between the bacterial cells, and between the bacteria and the surrounding matrix, lead to the complex phenomena observed in our experiments—in particular the observation that agarose concentration non-trivially affects the colony size at transition. Matching these approaches leads to a prediction for how the friction between the bacteria and the agarose should vary with agarose concentration. Our experimental conditions mimic numerous clinical and environmental scenarios in which bacteria invade soft matrices, as well as shedding more general light on the transition between two- and three-dimensional growth in multicellular assemblies. 相似文献
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56.
Pande R Xie L Zagozdzon-Wosik W Nesteruk K Wosik J 《Applied physics letters》2012,100(6):63701-637014
We report on investigations of nonlinear radiofrequency responses of electrolytes with Na(+) and Cl(-) ions placed within gold electrodes of a capacitor. The sample was part of a frequency-adjustable inductance-capacitance-resistance (LCR) parallel resonant circuit, and measurements were carried out using the two frequencies intermodulation distortion technique. We employed double layer model to analyze the observed nonlinearities and their dependence on ionic concentration. Electrode-electrolyte interface polarization was found to be a predominant cause of this intrinsic nonlinearity and to be dependent on electrolytic ion concentration. We also measured and calculated coefficients of resistive and capacitive components of the observed nonlinearity. 相似文献
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58.
Krzysztof Dmitruk Marcin Denkowski Michał Mazur Paweł Mikołajczak 《Signal, Image and Video Processing》2017,11(4):613-620
Dual-energy X-ray imaging has a vast range of application in security. Luggage inspection is an essential process for an airplane or court house security as well as securing mass events. An image of a content of some package may help to figure out if there is any dangerous object inside and avoid possibly threatening situation. As the raw X-ray images are not always easy to analyze and interpret, some image processing methods like an object detection, a frequency resolution increase or a pseudocoloring are being used. Since color can be a powerful tool to improve the usefulness of an information display, we propose pseudocoloring improvement by modifying material-based approach with edge detection to fill and sharpen color layers over the image making it easier to read and analyze. We demonstrate the effectiveness of the methods using real data, acquired from a professional dual-energy X-ray scanner. 相似文献
59.
This paper examines the relationship between innovation performance and employment growth in firms by taking a closer look at specific innovation activities and industry effects in the context of the services sector. Firm-level CIS data on Polish services firms in 2004–2009 are analysed using robust M-estimation. The results indicate that the effects of product, process and organisational innovations depend strongly on the level of technological opportunities in the industry in question. Given the widely acknowledged role of marketing innovations in services, possible synergies between innovations in the form of new products and new marketing techniques are also analysed. We demonstrate that marketing innovations are conducive to firm growth if they complement product innovations, but they are less likely to foster growth when applied in isolation. 相似文献
60.
Tianwu Wang Krzysztof Iwaszczuk Emil Astrup Wrisberg Emil Vosmar Denning Peter Uhd Jepsen 《Journal of Infrared, Millimeter and Terahertz Waves》2016,37(6):592-604
The performance of air-biased coherent detection (ABCD) in a broadband two-color laser-induced air plasma system for terahertz time-domain spectroscopy (THz-TDS) has been investigated. Fundamental parameters of the ABCD detection, including signal-to-noise ratio (SNR), dynamic range (DR), and linearity of detection have been characterized. Moreover, the performance of a photomultiplier tube (PMT) and an avalanche photodiode (APD) as photodetector in the ABCD have been compared. We have observed nonlinear behavior of PMT detector, which leads to artificial gain factor in TDS spectroscopy. The APD turns out to have superior linearity and three times higher dynamic compared to the PMT. 相似文献