Integrated lectin affinity microfluidic chip for glycoform separation

Lectin affinity chromatography was miniaturized into a microfluidic format, which results in improvement of performance, as compared to the conventional method. A lectin affinity monolith column was prepared in the microchannel of a microfluidic chip. The porous monolith was fabricated by UV-initiated polymerization of ethylene dimethacrylate (EDMA) and glycidyl methacrylate (GMA) in the presence of porogeneities, followed by immobilization of pisum sativum agglutinin (PSA) on the monolith matrix. Using electroosmosis as the driven force, lectin affinity chromatographies of three kinds of glycoprotein, turkey ovalbumin (TO), chicken ovalbumin (CO), and ovomucoid (OM), were carried out on the microfluidic system. All the glycoproteins were successfully separated into several fractions with different affinities toward the immobilized PSA. The integrated system reduces the time required for the lectin affinity chromatography reaction to approximately 3%, thus, the overall analysis time from 4 h to 400 s. Only 300 pg of glycoprotein is required for the whole separation process. Moreover, troublesome operations for lectin affinity chromatography are simplified.     

Extraction of cesium ions from aqueous solutions using calix[4]arene-bis(tert-octylbenzo-crown-6) in ionic liquids

Solvent extraction of cesium ions from aqueous solution to hydrophobic ionic liquids without the introduction of an organophilic anion in the aqueous phase was demonstrated using calix[4]arene-bis(tert-octylbenzo-crown-6) (BOBCalixC6) as an extractant. The selectivity of this extraction process toward cesium ions and the use of a sacrificial cation exchanger (NaBPh(4)) to control loss of imidazolium cation to the aqueous solutions by ion exchange have been investigated.     

Excitation of surface-plasmon polaritons by use of a zeroth-order Bessel beam

We report an experimental result that shows the excitation of surface plasmon polaritons by use of a zeroth-order Bessel beam. From the viewpoint of energy efficiency, the zeroth order Bessel beam is ideal for the local excitation of surface plasmon polaritons on a metal film. We introduce an optical setup using an axicon element in order to carry out the excitation.     

Spinning of the solutions of poly(vinyl alcohol)/NaCl/H2O systems and their fiber properties

The NaCl/water solutions of commercial poly(vinyl alcohol) (a-PVA) with the degrees of polymerization and saponification of 1730 and 99.39%, respectively were gel-spun into methanol (–30°C) and then the removed NaCl dry-filaments were drawn at 200°C. The draw ratio showed the maximum at NaCl concentration C _N = 3 wt% (polymer concentration C _P = 150 kg/m³). The Young's modulus and strength at break were 95 and 6 GPa ( = 19). In the case of DMSO system (C _P = 170 kg/m³), the Young's modulus was 52 GPa ( = 25) and the strength at break was 3.3 GPa. Under a polarized microscope, the fine lateral structure was observed for the DMSO system, but not for the NaCl system, that is, the smooth structure was recognized. The smooth structure, like a Netlon packing net for oranges, might be considered to result in heightening of mechanical properties.     

基于不完全数据的异常挖掘算法研究

异常挖掘是数据挖掘的重要研究内容之一,对于不完全数据会面对双重的困难．首先将用于缺失数据填充的EM算法和MI算法推广到混合缺失情形,并根据Weisberg的不完全数据填充理论,提出了RE算法,然后通过将聚类分析与向前搜索算法结合起来,获得了比单纯的向前搜索法更优越的算法．最后,在上述填充算法的基础上探讨了不完全数据的异常挖掘．理论和实例分析均表明,基于不完全数据的异常挖掘算法是有效可行的．     

Construction of feature-matching perception in virtual assembly

An important characteristic of virtual assembly is interaction.Traditional direct manipulation in virtual assembly relies on dynamic collision detection,which is very time-consuming and even impossible in desktop virtual assembly environment.Feature-matching is a critical process in harmonious virtual assembly,and is the premise of assembly constraint sensing.This paper puts forward an active object-based feature-matching perception mechanism and a feature-matching interactive computing process,both of which make the direct manipulation in virtual assembly break away from collision detection.They also help to enhance virtual environment understandability of user intention and promote interaction performance.Experimentl results show that this perception mechanism can ensure that users achieve real-time direct manipulation in desktop virtual environment.     

The estimation of absorbed doses received by a victim of a Chinese radiation accident

The aim of this work was to estimate absorbed doses received by a victim of the radiation accident with a 60Co source in Henan province, China. With a Monte Carlo stochastic simulation method, an estimation method for doses to the radiation accident victim was made. It utilised a mathematical model of adult man (MIRD) and a relative applied computer program was developed. By means of the simulated conditions of the accident, the absorbed doses to the victim's main organ and total body doses were estimated. The results estimated by our Monte Carlo method are close to those of experimental simulation measurement of the accident. With its convenience and rapidity, this method will be valuable for radiation dose reconstruction for victims in radiation accidents.     

Effects of atmospheric ozone on microarray data quality

A data anomaly was observed that affected the uniformity and reproducibility of fluorescent signal across DNA microarrays. Results from experimental sets designed to identify potential causes (from microarray production to array scanning) indicated that the anomaly was linked to a batch process; further work allowed us to localize the effect to the posthybridization array stringency washes. Ozone levels were monitored and highly correlated with the batch effect. Controlled exposures of microarrays to ozone confirmed this factor as the root cause, and we present data that show susceptibility of a class of cyanine dyes (e.g., Cy5, Alexa 647) to ozone levels as low as 5-10 ppb for periods as short as 10-30 s. Other cyanine dyes (e.g., Cy3, Alexa 555) were not significantly affected until higher ozone levels (> 100 ppb). To address this environmental effect, laboratory ozone levels should be kept below 2 ppb (e.g., with filters in HVAC) to achieve high quality microarray data.     

High-Average-Power Self-Starting Mode-Locked Ti:sapphire Laser With a Broadband Semiconductor Saturable-Absorber Mirror

We present a novel high-power, self-starting mode-locked Ti:sapphire laser with a semiconductor saturable-absorber mirror as the starter element. This laser, whose slope efficiency is 22%, generates pulses with an average power as high as 1.7 W and a pulse width as short as 16 fs. The self-starting process is also investigated.     

板仓坝王宅

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