Increased production of reactive oxygen species by rat liver mitochondria after chronic ethanol treatment

Rat liver microsomes and, to a lesser extent, nuclei were previously shown to produce reactive oxygen species at elevated rates after chronic ethanol treatment. The ability of intact rat liver mitochondria to interact with iron and either NADH or NADPH, and the effects of ethanol treatment, on production of reactive oxygen intermediates was determined. In the presence of ferric-ATP, NADH or NADPH catalyzed mitochondrial lipid peroxidation. Rates were elevated two- to threefold with mitochondria from ethanol-fed rats with both reductants. Mitochondrial lipid peroxidation was insensitive to superoxide dismutase, catalase, or hydroxyl radical scavengers but was sensitive to GSH and anti-oxidants such as trolox. Mitochondrial generation of hydroxyl radical-like species (assayed by oxidation of chemical scavengers) was increased after chronic ethanol treatment, as was H2O2 production. Modifiers of mitochondrial metabolism such as rotenone, cyanide, or an uncoupling agent, had no effect on mitochondrial production of reactive oxygen intermediates. The membrane-impermeable thiol reagent, p-chloromercuribenzoate, was complete inhibitory with both mitochondrial preparations. The activity of the rotenone-insensitive NADH-cytochrome c reductase, an enzyme of the outer mitochondrial membrane, was increased 40 to 60% by the ethanol treatment. These results suggest that NADH acting via the outer membrane NADH reductase can catalyze an iron-dependent production of oxygen radicals by rat liver mitochondria. The outer mitochondrial membrane fraction, prepared by digitonin fractionation, displayed increased rotenone-insensitive NADH-cytochrome c reductase activity after ethanol treatment and was more reactive in catalyzing scission of pBR322 DNA from the supercoiled form to the open circular forms. Rates of oxygen radical production by mitochondria and the extent of increase produced by chronic ethanol treatment are similar to those previously found with microsomes when NADH is the cofactor. Oxidation of ethanol by alcohol dehydrogenase generates NADH, and NADH-dependent production of reactive oxygen species by various organelles is increased after chronic ethanol treatment. These acute metabolic interactions coupled to induction by chronic ethanol treatment may play an important role in the development of a state of oxidative stress in the liver by ethanol.     

A Study of the Specific Heats of the Compounds in the Sm_2O_3-BaO-CuO System

By means of X-ray diffraction and adiabatic scanning calorimeter,the specific heatsof five compounds in the Sm_2O_3-BaO--CuO system,such as Sm_2BaO_4,Sm_2CuO_4,BaCuO_4,Sm_2BaCuO_5,SmBa_2Cu_3O_(7-x),from -150℃ to 800℃ have been measured.It is found that the specific heats ofthese compounds increase smoothly with the increase of temperature.     

对包装工程教育的认识及其本科教育模式的探索

在对高等工程教育本质以及存在问题进行分析的基础上，针对包装工程教育进行了深入探讨。并结合郑州大学包装工程专业的特点，对复合型人才培养的教育模式进行了探索与实践。     

巨型机转子机制的改进策略

本文分析了通用寄存器型计算机和通用堆栈型计算机处理转子指令的不同方法,研究了类Cray-1转子机制的实现原理,提出了在类Cray-1通用寄存器型模型机上采用堆栈来处理转子指令的实现方法。     

The treatment of peripapillary diverticula of the duodenum

An original operation is proposed for localization of the diverticulum in the descending portion of the duodenum which excludes traumas of the pancreas. Clinical observations are described.     

NiAl—TiC原位复合材料的室温韧化机制研究

通过热压放热反应合成工艺制备的ＮｉＡｌ－２００ｖｏｌ％ＴｉＣ原位复合材料，它的平均室温断裂韧性比单相ＮｉＡｌ提高了５０％，断口形貌及裂纹扩展路径观查表明，裂纹偏转机制是复合材料的主要韧化机制。     

浅谈我国旋转防喷器技术的现状与发展

介绍了我国旋转防喷器技术的发展现状，分析了几种不同类型旋转防喷器的结构特点，指出旋转防喷器的发展方向及其与国外同类技术之间的差距。     

改进灰色神经网络模型在电量预测中的应用

研究过程中分析比较了人工神经网络和灰色模型的优缺点,尝试将人工神经网络模型与改进灰色模型进行有机结合,从而提出了改进灰色神经网络模型。新的耦合方式发挥了灰色预测方法中累加生成的优点,便于神经网络进行训练,又避免了灰色预测方法带来的误差,提高了预测精度,是一种新的有益探索;为实际工程应用提供了重要的参考。     

童氏标准公式的校正及应用

在童标准公式中引入校正系数，提出了童氏校正公式，应用该校正公式可对水驱断块油田以及标定采收集较低的油田进行早期含水率预测和采收率预测，应用实例表明，与实际生产数据对比，校正公式计算值比标准公式计算值的误差小得多。