Glibenclamide for the Treatment of Ischemic and Hemorrhagic Stroke

Ischemic and hemorrhagic strokes are associated with severe functional disability and high mortality. Except for recombinant tissue plasminogen activator, therapies targeting the underlying pathophysiology of central nervous system (CNS) ischemia and hemorrhage are strikingly lacking. Sur1-regulated channels play essential roles in necrotic cell death and cerebral edema following ischemic insults, and in neuroinflammation after hemorrhagic injuries. Inhibiting endothelial, neuronal, astrocytic and oligodendroglial sulfonylurea receptor 1–transient receptor potential melastatin 4 (Sur1–Trpm4) channels and, in some cases, microglial K_ATP (Sur1–Kir6.2) channels, with glibenclamide is protective in a variety of contexts. Robust preclinical studies have shown that glibenclamide and other sulfonylurea agents reduce infarct volumes, edema and hemorrhagic conversion, and improve outcomes in rodent models of ischemic stroke. Retrospective studies suggest that diabetic patients on sulfonylurea drugs at stroke presentation fare better if they continue on drug. Additional laboratory investigations have implicated Sur1 in the pathophysiology of hemorrhagic CNS insults. In clinically relevant models of subarachnoid hemorrhage, glibenclamide reduces adverse neuroinflammatory and behavioral outcomes. Here, we provide an overview of the preclinical studies of glibenclamide therapy for CNS ischemia and hemorrhage, discuss the available data from clinical investigations, and conclude with promising preclinical results that suggest glibenclamide may be an effective therapeutic option for ischemic and hemorrhagic stroke.     

基于统计软件R的安全壳泄漏率试验数据有效性分析

安全壳泄漏率计算过程中，最重要的环节是以不同时刻测量数据对时间进行线性回归分析。对回归的显著性检验以及方差分析是评价试验结果有效性的重要手段。本文基于统计软件R对某电厂调试阶段安全壳泄漏率试验的数据进行分析，通过对线性回归模型的独立性、正态性和异方差性检验以及极端样本点的剔除等方式，探讨泄漏率计算前的回归诊断对计算结果可靠性的影响。通过回归诊断的实例分析发现，在安全壳泄漏率计算的数据样本中，可能存在自相关、非正态和异方差性等问题影响回归结果，进而影响泄漏率的最终结果。因此，在计算泄漏率结果时，须通过回归诊断方法评价数据的有效性，对不能通过检验的样本应通过适当方法对最终结果进行修正。      

Steels for Bridge Structures

Metallurgist - We consider domestic and foreign standards for rolled metals used in bridge building. Domestic standards contain elevated requirements to the reliability of rolled metals in terms of...     

Investigation into the Influence of Sodium Lignosulfonate,Anionic Surfactants,and Their Mixtures on the Copper Cementation Rate by Zinc

Russian Journal of Non-Ferrous Metals - This article is devoted to the influence of sodium lignosulfonate (SL), anionic surfactants (sodium dodecylsulfate (SDS), sodium dodecylbenzene sulfonate...     

Assembly of a DNA Origami Chinese Knot by Only 15% of the Staple Strands

As a giant leap in DNA self-assembly, DNA origami has exhibited an unprecedented ability to construct nanostructures with arbitrary shapes and sizes. In typical DNA origami, hundreds of short DNA staple strands fold a long, single-stranded (ss) DNA scaffold cooperatively into designed nanostructures. However, large numbers of DNA strands are expensive and would hinder applications such as pharmaceutical investigations because of the complicated components. Therefore, one challenge is how to reduce the number of staple strands needed to construct DNA origami. For a DNA origami structure, the scale-free folding pattern of the scaffold strand is determined by staple strands at the branching vertexes. Simple duplex regions help to define the size-related features of the origami geometry. In this study, we hypothesized that a scaffold strand can be correctly folded into a designed topology by using only staple strands involved in branching vertexes. After assembly, any remaining, flexible, single-stranded regions of the scaffold could be converted into rigid duplexes by DNA polymerase to achieve the designed geometric structures. To demonstrate the concept, we used only 18 staple strands (covering 15 % of the scaffold strand) to assemble a porous DNA nanostructure, which was visualized by atomic force microscopy (AFM). This study helps understanding of the role of cooperativity in origami folding, and provides a cost-effective approach for small-scale prototyping DNA origami.     

Oligomerization of 1-Pentene on Zeolite Catalysts

Catalysis in Industry - The oligomerization of 1-pentene is an efficient process for producing environmentally friendly high-quality fuel components. The catalytic properties of FAU, OFF, MOR, BEA,...     

Effect of Ball Milling Time on the Physical,Thermal and Fracture Behaviour of 2MgO.2Al2O3.5SiO2 Precursors

Silicon - In this study, an evaluation has been made into the influence of milling time on the thermo-physical and fracture properties of a MgO-Al2O3-SiO2 system. The powdery and compacted...     

多肽N端修饰方法研究进展

多肽是源于生物体内的一类重要的生物活性物质,但由于稳定性差等原因,大大限制了其应用。通过对多肽进行结构修饰,可以改变其理化性质,进而提高其稳定性及生物活性。综述了近年来多肽N端修饰方法的研究进展,包括乙酰化、PEG(聚乙二醇)修饰、荧光试剂修饰、多肽N端亲水性基团修饰、NCL及EPL连接反应等,介绍了2-氨基辛酸、丁烯酶1、N-脒基-焦谷氨酰苯丙氨酸、N-terminal caps等多肽N端修饰实例。