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111.
The essential fatty acid arachidonate is oxidized by cytochrome P-450 epoxygenases to four epoxyeicosatrienoic acids (EETs): 14,15-, 11,12-, 8,9-, and 5,6-EETs. Each of the four EET regioisomers and their hydrolysis products (DHETs) has multiple paracrine and autocrine functions and may also potently dilate blood vessels and activate potassium channels. The present work describes a method to resolve EETs and DHETs by capillary electrophoresis (CE) using trimethyl-beta-cyclodextrin and CH3CN as buffer additives. While stored at 25 degrees C, most of the EET and DHET regioisomers remained intact when suspended in alkaline vehicle. However, under these same conditions, 5,6-EET rapidly broke down to a lactone and was slowly converted to 5,6-DHET. When subjected to CE, the EET and DHET regioisomers were baseline resolved (R > or = 1.3); 10 pg of an EET or a DHET regioisomer was readily detectable at 194 nm. In addition, the UV spectra were regiospecific and identical to those obtained during HPLC except that an additional, weak absorption occurred at 235 nm. Together, the high-sensitivity, high-resolution, and differential UV spectra permitted the identification and quantification of EETs in phospholipids isolated from murine liver. Thus, CE was successfully used for the trace analysis of eicosanoids. 相似文献
112.
We examine the light-activation properties of micrometer-sized gear structures fabricated with polysilicon surface micromachining techniques. The gears are held in place on a substrate through a capped anchor post and are free to rotate about the post. The light-activation technique is modeled on photon radiation pressure, and the equation of motion of the gear is solved for this activation technique. Experimental measurements of torque and damping are found to be consistent with expected results for micrometer-scale devices. Design optimization for optically actuated microstructures is discussed. 相似文献
113.
Miniature cylindrical ion trap mass spectrometer 总被引:4,自引:0,他引:4
Patterson GE Guymon AJ Riter LS Everly M Griep-Raming J Laughlin BC Ouyang Z Cooks RG 《Analytical chemistry》2002,74(24):6145-6153
A miniature cylindrical ion trap mass spectrometer is described, and preliminary data are presented. Functionality and performance of laboratory-scale instruments have been maintained to the extent possible in this battery-operated mass spectrometer. Capabilities include tandem mass spectrometry experiments. Custom-designed electronic components include the RF scanning and amplification system, data acquisition components, and lens power supplies, as well as a custom-software application. Direct leak and membrane introduction inlet systems are used for sample introduction. A mass/charge range of approximately 250 Th with unit mass resolution has been demonstrated. 相似文献
114.
115.
Sharifah Syed-Abdullah Mike Holcombe Marian Gheorge 《Empirical Software Engineering》2006,11(1):143-167
This paper describes an empirical study, which addresses the aspect of well being amongst members of the software development
teams. The question of interest is whether an agile methodology has any distinct effect on the well being of the software
developers. Both quantitative and qualitative methods were utilised, including the participative observation, focus group
interviews, close-ended questionnaires and simple statistical tests such as Spearman Correlation and Mann–Whitney test. Initial
results showed that an agile methodology (XP) has a positive effect on the level of enthusiasm of the software developers
in the most dynamic project. To understand why XP can increase enthusiasm, results are interpreted with references to cognitive,
affective and managerial properties of the practices studied. This result needs further investigation on the individual effects
of each practice on the wellbeing and attitudes of Software Engineering (SE) teams. 相似文献
116.
117.
Tight lower bounds for certain parameterized NP-hard problems 总被引:1,自引:0,他引:1
Jianer Chen Benny Chor Mike Fellows Xiuzhen Huang David Juedes Iyad A. Kanj Ge Xia 《Information and Computation》2005,201(2):216-231
Based on the framework of parameterized complexity theory, we derive tight lower bounds on the computational complexity for a number of well-known NP-hard problems. We start by proving a general result, namely that the parameterized weighted satisfiability problem on depth-t circuits cannot be solved in time no(k)mO(1), where n is the circuit input length, m is the circuit size, and k is the parameter, unless the (t − 1)-st level W[t − 1] of the W-hierarchy collapses to FPT. By refining this technique, we prove that a group of parameterized NP-hard problems, including weighted sat, hitting set, set cover, and feature set, cannot be solved in time no(k)mO(1), where n is the size of the universal set from which the k elements are to be selected and m is the instance size, unless the first level W[1] of the W-hierarchy collapses to FPT. We also prove that another group of parameterized problems which includes weighted q-sat (for any fixed q 2), clique, independent set, and dominating set, cannot be solved in time no(k) unless all search problems in the syntactic class SNP, introduced by Papadimitriou and Yannakakis, are solvable in subexponential time. Note that all these parameterized problems have trivial algorithms of running time either nkmO(1) or O(nk). 相似文献
118.
The linear minimum mean-squared error (LMMSE) channel estimation for orthogonal frequency-division multiplexing (OFDM) systems requires a large number of complex multiplications. We evaluate a simplified LMMSE channel estimation algorithm in a transmit diversity environment by applying a significant weight catching (SWC) technique to the LMMSE fixed weighting matrix. The SWC technique itself is based on modifying the smoothing matrix by leaving the Γ largest values in each row and turning the rest to zeros. This allows the computational complexity of the full LMMSE processor to be reduced by more than 50%. In the well known LMMSE by singular value decomposition (SVD) technique the sparse approximation is accomplished by zeroing out all but the r largest singular values. LMMSE by SVD is the preferred approximation technique for low delay spread channels. However, in channels with large delay spreads, LMMSE by SWC is a better choice in terms of computational complexity and estimation accuracy
Igor Tolochkoreceived his Dipl.-Eng. Degree in Electrical Engineering from Polytechnic Institute, Riga, Latvia in 1987 and PhD from Victoria University, Melbourne, Australia in 2005. He was a senior and later principal design engineer in mobile communications at the Riga Semiconductor Institute Alpha (1988 – 1993). During 1993 – 1998, he was involved in research and development activities in communications with different companies in Riga and Melbourne, Australia. From 1998 to 2002, he was with Ericsson Australia as a senior design engineer. Currently, he works for NEC Australia Pty. Ltd. as a senior design engineer in 3G Mobile Department. His current research interests include digital signal processing, indoor and outdoor wireless communications and error control coding.
Michael Faulkner(M'84) received the B.Sc. (Eng) from Queen Mary College, London University, UK, in 1970, the M.E. degree from the University of New South Wales, Australia in 1978, and the PhD from University of Technology Sydney in 1993. From 1972 to 1975 he was with STC (now Alcatel) Australia. From 1975 to 1977 he as with the University of New South Wales, and since then as a lecturer and now professor at Victoria University of Technology, Melbourne, Australia where he is director of the Telecommunications and Micro-electronics research centre. Between 1988 and 2000 he spent four periods at Lund University, Sweden. He was co-recipient of the IEE's 1997 IERE prize for a paper on amplifier linearisation. His current interests are, signal processing, radio technology, radio systems and MIMO/OFDM. 相似文献
119.
Shahram Izadi Mike Fraser Steve Benford Martin Flintham Chris Greenhalgh Tom Rodden Holger Schnädelbach 《Personal and Ubiquitous Computing》2002,6(4):290-298
The Citywide project is exploring ways in which technology can provide people with rich and engaging digital experiences
as they move through physical space, including historical experiences, performances and games. This paper describes some initial
results and experiences with this project based upon two prototype demonstrators. In the first, we describe an application
in which a search party explores an archaeological site, uncovering enacted scenes within the virtual world that are of a
historical relevance to their particular physical location. In the second, we describe a museum experience where participants
explore an outdoors location, hunting for buried virtual artifacts that they then bring back to a museum for a more detailed
study. Our demonstrators employ a varied set of devices, including mobile wireless interfaces for locating hotspots of virtual
activity when outdoors, to give different experiences of the virtual world depending upon location, task, available equipment
and accuracy of tracking. We conclude by discussing some of the potential advantages of using an underlying shared virtual
world to support interactive experiences across extended physical settings. 相似文献
120.