Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for plasmid DNA purification from Escherichia coli lysate

The aim of this study is to prepare poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine) [PHEMAH] magnetic nanoparticles for plasmid DNA (pDNA) purification from Escherichia coli (E. coli) cell lysate. Magnetic nanoparticles were produced by surfactant free emulsion polymerization. mPHEMAH nanoparticles were characterized by elemental analysis, Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), vibrating sample magnetometer (VSM), electron spin resonance (ESR), thermogravimetric analyses (TGA) and transmission electron microscopy (TEM). Surface area, average particle size and size distribution were also performed. Specific surface area of the mPHEMAH nanoparticles was found to be 1180 m²/g. Elemental analysis of MAH for nitrogen was estimated as 0.18 mmol/g polymer. The amount of pDNA adsorbed onto the mPHEMAH nanoparticles first increased and then reached a saturation value at around 1.0 mg/mL of pDNA concentration. Compared with the mPHEMA nanoparticles (50 μg/g polymer), the pDNA adsorption capacity of the mPHEMAH nanoparticles (154 mg/g polymer) was improved significantly due to the MAH incorporation into the polymeric matrix. The maximum pDNA adsorption was achieved at 25 °C. The overall recovery of pDNA was calculated as 92%. The mPHEMAH nanoparticles could be used six times without decreasing the pDNA adsorption capacity significantly. The results indicate that the PHEMAH nanoparticles promise high selectivity for pDNA.     

基于BP神经网络的MMC电容电压控制研究

模块化多电平换流器(Modular Multilevel Converter,MMC)作为一种新型的电压源换流器,被广泛应用于直流输电领域.电容的电压平衡问题在MMC中非常重要,随着MMC子模块个数的增多,电容电压的平衡变得越来越困难.本文提出了一种基于BP-PID的双闭环控制策略,通过控制调制波来控制子模块的工作状态...     

一种基于单换能器的超声离合器的设计及性能分析

提出了一种基于单换能器的新型超声离合器;分析了近场超声悬浮的机理,设计并制造了超声换能器和预紧装置,构建了超声离合器原型机;对该超声离合器的功率-转速、功率-扭矩和功率-减速比特性进行了实验研究。结果表明,原型机实现完全分离的功率为0.35kW;电机转速为500r/min和800r/min下原型机的极限扭矩分别为0.39N·m和0.42N·m。验证了基于单换能器的超声离合器的可行性和有效性。     

The first catalytic hydrolysis of ethylenediamine bisborane with hydrogel-supported metallic nanoparticles

Ethylene diamine bisborane (EDB) was synthesized in a single step as the hydrogen storage material. The synthesized compound was firstly used in the literature for the production of hydrogen gas by catalytic hydrolysis reaction. Cu, Co and Ni nanoparticles with average sizes of 75–150 nm formed in p(acrylicacid-co-vinylimidazole) hydrogel network structures were used as catalysts for the hydrolysis reaction. The effect of the parameters such as catalyst type, EDB concentration, catalyst concentration, temperature and solvent environment on the catalytic hydrolysis reaction of EDB was investigated. In the activity tests for the catalyst, it was determined that the catalyst had a loss of only 15% in activity even at the end of 5 cycles. The activation energies of hydrolysis reaction were calculated as 39.42 kJmol^–1, 44.77 kJmol^–1 and 47.48 kJmol^–1 for Cu, Co and Ni hydrogel composite catalyst, respectively.     

Assessment of deformation modulus of weak rock masses from pressuremeter tests and seismic surveys

The deformation modulus of intact rock can be determined through standardized laboratory tests for heavily jointed rock masses but this is very difficult, while in situ tests are time-consuming and expensive. In this study, the deformation modulus of selected heavily jointed, sheared and/or blocky, weathered, weak greywacke, andesite and claystone were assessed, based on pressuremeter tests, geo-engineering characterization and seismic surveys. Empirical equations based on GSI and RMR values are proposed to indirectly estimate the deformation modulus of the greywackes. For the andesites, the spacing of the discontinuities is greater than the length of the pressuremeter probe hence the intact rather than rock mass deformation modulus is obtained. The pressuremeter test results from the claystones could not be correlated with the field data; the relationship between the ratio of rock mass modulus to intact rock modulus and RQD appears to give a better estimation of the deformation modulus.      

Comparison of the structural and magnetic properties of submicron barium hexaferrite powders prepared by molten salt and solid state calcination routes

The effect of crystallinity and particle morphology of the submicron barium hexaferrite (BaFe₁₂O₁₉) powders on the magnetic properties was investigated on powders synthesized by solid-state calcination (BHF-c) and molten salt synthesis (BHF-m) methods. Solid-state calcination route was found to yield agglomerated powders with poor crystallinity, whereas molten salt synthesis resulted in well crystallized powders with an anisometric morphology. The saturation magnetization of the BHF-m and BHF-c samples is 59 emu/g, and 56 emu/g at 300 K, and 90 emu/g, and 86 emu/g at 10 K. The temperature dependence of magnetization of the BHF-m is higher and the increase in magnetocrystal anisotropy with decreasing temperature is also steeper than that of the BHF-c due to the higher crystallinity. The magnetocrystalline anisotropy constant, K, calculated from the Stoner–Wohlfarth theory, of the BHF-m and BHF-c powders is 14.24 and 10.14 HA²/kg, respectively. The higher effective anisotropy, K_eff of the BHF-m is also confirmed through ferromagnetic resonance measurements. In conclusion, the higher crystallinity, slightly higher particle size and anisometric morphology of the BHF-m particles translated into higher magnetic properties and magnetocrystalline anisotropy.     

Property enhancement in unsaturated polyester nanocomposites by using a reactive intercalant for clay modification

Polymeric nanocomposites were synthesized from unsaturated polyester (UPE) matrix and montmorillonite (MMT) clay using an in situ free radical polymerization reaction. Organophilic MMT was obtained using a quaternary salt of coco amine as intercalant having a styryl group making it a reactive intercalant. The resultant nanocomposites were characterized via X‐ray diffraction and transmission electron microscopy. The effect of increased nanofiller loading on the thermal and mechanical properties of the nanocomposites was investigated. All the nanocomposites were found to have improved thermal and mechanical properties as compared with neat UPE matrix, resulting from the contribution of nanolayer connected intercalant‐to‐crosslinker which allows a crosslinking reaction. It was found that the partially exfoliated nanocomposite structure with an exfoliation dominant morphology was achieved when the MMT loading was 1 wt %. This nanocomposite exhibited the highest thermal stability, the best dynamic mechanical performance and the highest crosslinking density, most probably due to more homogeneous dispersion and optimum amount of styrene monomer molecules inside and outside the MMT layers at 1 wt % loading. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013     

Drift estimation using pairwise slope with minimum variance in wireless sensor networks

Time synchronization is mandatory for applications and services in wireless sensor networks which demand common notion of time. If synchronization to stable time sources such as Coordinated Universal Time (UTC) is required, employing the method of flooding in order to provide time synchronization becomes crucial. In flooding based time synchronization protocols, current time information of a reference node is periodically flooded into the network. Sensor nodes collect the time information of the reference node and perform least-squares regression in order to estimate the reference time. However, least-squares regression exhibits a poor performance since sensor nodes far away from the reference node collect the time information with large deviations. Due to this fact, the slopes of their least-squares line exhibit large errors and instabilities. As a consequence, the reference time estimates of these nodes also exhibit large errors.This paper proposes a new slope estimation strategy for linear regression to be used by flooding based time synchronization protocols. The proposed method, namely Pairwise Slope With Minimum Variance (PSMV), calculates the slope of the estimated regression line by considering the pairwise slope between the earliest and the most recently collected data points. The PSMV slope is less affected by the large errors on the received data, i.e. it is more stable, and it is more computationally efficient when compared to the slope of the least-squares line. We incorporated PSMV into two flooding based time synchronization protocols, namely Flooding Time Synchronization Protocol (FTSP) and PulseSync. Experimental results collected from a testbed setup including 20 sensor nodes show that PSMV strategy improves the performance of FTSP by a factor of 4 and preserves the performance of PulseSync in terms of synchronization error with 40% less CPU overhead for linear regression. Our simulations show that these results also hold for networks with larger diameters and densities.     

WC-17Co粉末密度对涂层力学性能及残余应力的影响

选取3种不同密度的WC-17Co粉末,采用超音速火焰喷涂法制备厚度为0.3 mm的涂层。通过扫描电镜观察分析了3种涂层的孔隙率,采用压痕法测量了涂层的努氏硬度与弹性模量,同时采用剥层法对不同密度粉末制备的WC-17Co涂层残余应力进行了测试与计算。结果表明,涂层孔隙率随WC-17Co粉末密度的增大而增大,涂层的努氏硬度、弹性模量均随WC-17Co粉末密度的增大而减小。WC-17Co涂层内部存在的残余应力表现为压应力,且应力值随涂层厚度的增大而增加,在临近涂层-基体界面处迅速减小。涂层残余压应力最大值随WC-17Co粉末密度的增大而增加：粉末密度为11.52、12.86、13.49 g·cm^-3所制备的涂层残余应力最大值分别为-798、 -986和-1120 MPa。