Both Specific Endothelial and Proximal Tubular Adam17 Deletion Protect against Diabetic Nephropathy

ADAM17 is a disintegrin and metalloproteinase capable of cleaving the ectodomains of a diverse variety of molecules including TNF-α, TGF-α, L-selectin, and ACE2. We have previously demonstrated that renal ADAM17 is upregulated in diabetic mice. The role of endothelial (eAdam17) and proximal tubular (tAdam17) Adam17 deletion in renal histology, modulation of the renin angiotensin system (RAS), renal inflammation, and fibrosis was studied in a mouse model of type 1 Diabetes Mellitus. Moreover, the effect of Adam17 deletion in an in vitro 3D cell culture from human proximal tubular cells under high glucose conditions was evaluated. eAdam17 deletion attenuates renal fibrosis and inflammation, whereas tAdam17 deletion decreases podocyte loss, attenuates the RAS, and decreases macrophage infiltration, α-SMA and collagen accumulation. The 3D in vitro cell culture reinforced the findings obtained in tAdam17KO mice with decreased fibrosis in the Adam17 knockout spheroids. In conclusion, Adam17 deletion either in the endothelial or the tubular cells mitigates kidney injury in the diabetic mice by targeting different pathways. The manipulation of Adam17 should be considered as a therapeutic strategy for treating DN.     

The first learning track of the international planning competition

The International Planning Competition is a biennial event organized in the context of the International Conference on Automated Planning and Scheduling. The 2008 competition included, for the first time, a learning track for comparing approaches for improving automated planners via learning. In this paper, we describe the structure of the learning track, the planning domains used for evaluation, the participating systems, the results, and our observations. Towards supporting the goal of domain-independent learning, one of the key features of the competition was to disallow any code changes or parameter tweaks after the training domains were revealed to the participants. The competition results show that at this stage no learning for planning system outperforms state-of-the-art planners in a domain independent manner across a wide range of domains. However, they appear to be close to providing such performance. Evaluating learning for planning systems in a blind competition raises important questions concerning criteria that should be taken into account in future competitions.     

Environmental impact of castor oil catalytic transfer hydrogenation

In the vegetable oil chemical industry, hydrogenation is one of the most important processes. An alternative method for vegetable oil hydrogenation is the use of catalytic transfer hydrogenation (CTH), which can utilize organic molecules as hydrogen donors at ambient pressure. These alternative processes should be optimized in relation to the variables required for a good conversion and impacts should also be known to be minimized. An assessment of the environmental impact of laboratory scale chemical processes is an important tool to improve the technological aspects of a process (increased yields, reduced production times, lower costs) and it can also lead to the creation of a cleaner technology. Using the Leopold Matrix, we have succeeded in developing a more efficient and cleaner process for the CTH of castor oil using Raney Ni as a catalyst and cyclohexene or isopropanol as a hydrogen donor solvent. The results of the technical and environmental assessments showed that the extent of conversion for the unsaturation reaction was high (>99 %), and the environmental impact of the process could be significantly reduced to create a cleaner technology. It was found, after process optimization, that the remaining environmental impacts were negative (67.48 %), local (78.95 %), temporary (95.33 %), direct (80.12 %), and reversible (95.32 %).     

Nafion/polyaniline/silica composite membranes for direct methanol fuel cell application

This work investigates the characterization and performance of polyaniline and silica modified Nafion membranes. The aniline monomers are synthesized in situ to form a polyaniline film, whilst silica is embedded into the Nafion matrix by the polycondensation of tetraethylorthosilicate. The physicochemical properties are studied by means of X-ray diffraction and Fourier transform infrared techniques and show that the polyaniline layer is formed on the Nafion surface and improves the structural properties of Nafion in methanol solution. Nafion loses its crystallinity once exposed to water and ethanol, whilst the polyaniline modification allows crystallinity to be maintained under similar conditions. By contrast, the proton conductivities of polyaniline modified membranes are 3–5-fold lower than that of Nafion. On a positive note, methanol crossover is reduced by over two orders of magnitude, as verified by crossover limiting current analysis. The polyaniline modification allows the membrane to become less hydrophilic, which explains the lower proton conductivity. No major advantages are observed by embedding silica into the Nafion matrix. The performance of a membrane electrode assembly (MEA) using commercial catalysts and polyaniline modified membranes in a cell gives a peak power of 8 mW cm⁻² at 20 °C with 2 M methanol and air feeding. This performance correlates to half that of MEAs using Nafion, though the membrane modification leads to a robust material that may allow operation at high methanol concentration.     

Effect of Strong Anisotropy in Grain-Boundary Energy on Boundary Mobility in Abnormally Grown Grains

The growth of a large crystal into a fine-grained polycrystal of the same phase is considered through a two-dimensional model. The macroscopic driving force for growth depends only on the average energy of the grain boundaries of the polycrystalline matrix. The analysis reveals a dependence of the local driving force and effective mobility on the orientation of the interface between the large crystal and the matrix. The orientations with very low energy relative to the neighboring orientations also have low effective mobility. Applications are found in modeling of abnormal grain growth in ceramics and in experiments designed to measure the mobility of grain boundaries.     

Coralline Skeleton Biomaterial Reduces Phagocytosis in Mouse Blood in vitro

Inflammatory and immunogenic response to foreign bodies presents a challenge in the use of biomaterials as implants for tissue restoration. Therefore, there is a need to understand the interactions between such implants and the blood. One such material, currently in clinical use for bone replacement in humans, is the skeleton of corals, in the form of crystalline aragonite. This biomaterial has been shown to impart a protective and supportive influence on several types of cells ex vivo. The carbonate skeleton activates secretion in phagocytes in vitro, however its effects on these cells in the blood, and on the process of phagocytosis itself, remain unknown. Using 1–500 μm particles of coral skeleton, we show that these particles bind blood proteins and alter the leukocyte population, reducing the proportion of granulocytes by more than 3-fold with no effect on the proportion of monocytes. In addition, the presence of coral skeleton in the blood causes a reduction in phagocytosis. Specifically, we observed a decrease in the percentage of phagocytic cells by 27 % in the granulocytes and by 73 % in monocyte family, as well as a 41.6 % reduction in the MFI of granulocytes, but with no such effect on monocytes. Taken together, the results suggest that the coral skeleton biomaterial may act as a strong, promotive scaffold for tissue regeneration due to its ability to reduce its rejection by inflammatory reactions such as phagocytosis.     

Effect of Heat Processing on IgE Reactivity and Cross‐Reactivity of Tropomyosin and Other Allergens of Asia‐Pacific Mollusc Species: Identification of Novel Sydney Rock Oyster Tropomyosin Sac g 1

1 Scope

Shellfish allergy is an increasing global health priority, frequently affecting adults. Molluscs are an important shellfish group causing food allergy but knowledge of their allergens and cross‐reactivity is limited. Optimal diagnosis of mollusc allergy enabling accurate advice on food avoidance is difficult. Allergens of four frequently ingested Asia‐Pacific molluscs are characterized: Sydney rock oyster (Saccostrea glomerata), blue mussel (Mytilus edulis), saucer scallop (Amusium balloti), and southern calamari (Sepioteuthis australis), examining cross‐reactivity between species and with blue swimmer crab tropomyosin, Por p 1.

2 Methods and results

IgE ELISA showed that cooking increased IgE reactivity of mollusc extracts and basophil activation confirmed biologically relevant IgE reactivity. Immunoblotting demonstrated strong IgE reactivity of several proteins including one corresponding to heat‐stable tropomyosin in all species (37–40 kDa). IgE‐reactive Sydney rock oyster proteins were identified by mass spectrometry, and the novel major oyster tropomyosin allergen was cloned, sequenced, and designated Sac g 1 by the IUIS. Oyster extracts showed highest IgE cross‐reactivity with other molluscs, while mussel cross‐reactivity was weakest. Inhibition immunoblotting demonstrated high cross‐reactivity between tropomyosins of mollusc and crustacean species.

3 Conclusion

These findings inform novel approaches for reliable diagnosis and improved management of mollusc allergy.     

Item selection counts: A comparison of empirical key and rational scale validities in theory-based and non-theory-based item pools.

Little explicit attention has been given to the impact of item pools on the validities and cross-validities of different background data scoring approaches. This study tests the idea that pools of items theoretically related to the performance of interest will outperform pools of items with no hypothesized relationship with the criterion. Validities and cross-validities of rational scales and empirical keys created from theory- and non-theory-based item pools were compared for 3 criteria. When size of the item pools was held constant, theory-based empirical keys (correlational and vertical percent) and rational scales showed larger validities and cross-validities than non-theory-based empirical keys (correlational and vertical percent) and showed minimal shrinkage in cross-validities. Even when item pool for the non-theory-based keys was expanded to include all items in the instrument, the theory-based keys showed comparable or slightly better validities and cross-validities for 2 of the 3 criteria, including college GPA, which was separated from the predictors by 4 years. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Reasoning with examples: propositional formulae and database dependencies

For humans, looking at how concrete examples behave is an intuitive way of deriving conclusions. The drawback with this method is that it does not necessarily give the correct results. However, under certain conditions example-based deduction can be used to obtain a correct and complete inference procedure. This is the case for Boolean formulae (reasoning with models) and for certain types of database integrity constraints (the use of Armstrong relations). We show that these approaches are closely related, and use the relationship to prove new results about the existence and size of Armstrong relations for Boolean dependencies. Furthermore, we exhibit close relations between the questions of finding keys in relational databases and that of finding abductive explanations. Further applications of the correspondence between these two approaches are also discussed. Received: 19 June 1995 / 31 August 1998