Scanning Tunneling Microscopy and Spectroscopy of Novel Silver–Containing DNA Molecules

The quest for a suitable molecule to pave the way to molecular nanoelectronics has been met with obstacles for over a decade. Candidate molecules such as carbon nanotubes lack the appealing trait of self‐assembly, while DNA seems to lack the desirable feature of conductivity. Silver‐containing poly(dG)–poly(dC) DNA (E‐DNA) molecules have recently been reported as promising candidates for molecular electronics, owing to the selectivity of their metallization, their thin and uniform structure, their resistance to deformation, and their maximum possible high conductivity. Ultrahigh vacuum (UHV) scanning tunneling microscopy (STM) of E‐DNA presents an elaborate high‐resolution morphology characterization of these unique molecules, along with a detailed depiction of their electronic level structure. The energy levels found for E‐DNA indicate a novel truly hybrid metal–molecule structure, potentially more conductive than other DNA‐based alternatives.     

数字工厂--数字制造教学与研究中的技术个案

１IntroductionWiththeimpactofinformationtechnologyandcomputernetworking熏thecoreofmanufacturingactivitieshasshiftedfromphysicalproductiontosystematicprocessingofinfor鄄mation眼１演．Tocaterforthischange熏manufacturerstodaymustbewellversedwiththecomputerapplicationsinmanufacturing．Thiscoupleswiththeadventofhigh－resolu鄄tiongraphics熏high－speedcomputing熏anduserintegrationdevices鸦virtualmanufacturinghasemergedasamajornewtechnologyinrecentyears眼２演．Theimpactforthechangeisparticularlypromin…     

Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide‐Modified Quantitative PCR

Biofilms containing Candida albicans are responsible for a wide variety of clinical infections. The protective effects of the biofilm matrix, the low metabolic activity of microorganisms within a biofilm and their high mutation rate, significantly enhance the resistance of biofilms to conventional antimicrobial treatments. Peptoids are peptide‐mimics that share many features of host defence antimicrobial peptides but have increased resistance to proteases and therefore have better stability in vivo. The activity of a library of peptoids was tested against monospecies and polymicrobial bacterial/fungal biofilms. Selected peptoids showed significant bactericidal and fungicidal activity against the polymicrobial biofilms. This coupled with low cytotoxicity suggests that peptoids could offer a new option for the treatment of clinically relevant polymicrobial infections.     

A simplified procedure for determining indoor daylight illuminance using daylight coefficient concept

The first step in evaluating the visual performance and energy efficiency provided by daylight requires an accurate estimation of the amount of daylight entering a building. The actual daylight illuminance of a room is mainly influenced by the luminance levels and patterns of the sky in the direction of view of the window at that time. The daylight coefficient concept, which considers the changes in the luminance of the sky elements, offers a more effective way of computing indoor daylight illuminances. Recently, Kittler et al. have proposed a new range of 15 standard sky luminance distributions including the CIE (International Commission on Illumination) standard clear sky. Lately, these 15 sky luminance models have been adopted as the CIE Standard General Skies. This paper presents a graphical method to calculate interior illuminance for the CIE standard clear sky using the daylight coefficient approach. The simplified techniques in the form of a nomograph and Waldram diagram were established and described. The performance of the proposed approach was evaluated against the results obtained by an independent calculation approach and a computer simulation program. It was shown that the daylight illuminances estimated by our graphical tool were in reasonably good agreement with those produced from the other two methods. The findings provide building professionals and students a reliable and simple alternative that incorporates the daylight coefficient concept to estimate the interior daylight illuminance and assess daylighting performance.     

An analysis of daylighting performance for office buildings in Hong Kong

Daylighting has often been recognized as a useful source of energy savings and visual comforts in buildings. Occupants expect good daylight in their working spaces. The quality and quantity of natural light entering a building depend on both internal and external factors. In Hong Kong, commercial building accounts for the major building energy use and electric lighting is one of the major electricity-consuming items. This paper studies the daylighting performance and energy implications for office buildings. A total of 35 commercial buildings have been selected in the survey. Key building parameters affecting daylighting designs are presented. Two typical office blocks were further analysed based on a lighting simulation program. The daylighting performance was evaluated in terms of daylight factor, room depth and glare index. It has been found that the daylighting performance for office buildings is quite effective. About one-third of the office areas that are near the perimeter regions have an average daylight factor of 5%. For inner region of deep plan offices, some innovative daylighting systems such as light redirecting panels and light pipe could be used to improve the daylighting performance. In general, the office building envelop designs are conducive to effective daylighting and proper daylight linked lighting controls could save over 25% of the total electric lighting use.     

A carbon nanotube needle biosensor

A carbon nanotube needle biosensor was developed to provide fast, cost effective and highly sensitive electrochemical detection of biomolecules. The sensor was fabricated based on an array of aligned multi-wall carbon nanotubes synthesized by chemical vapor deposition. A bundle of nanotubes in the array was welded onto the tip of a tungsten needle under a microscope. The needle was then encased in glass and a polymer coating leaving only the tip of the needle exposed. Cyclic voltammetry was performed to examine the redox behavior of the nanotube needle. The cyclic voltammetry results showed a steady-state response attributable to radial diffusion with a high steady-state current density. An amperometric sensor was then developed for glucose detection by physically attaching glucose oxidase on the nanotube needle. The amperometric response of these nanotube needles showed a high sensitivity with a low detection limit. It is expected that the nanotube needle can be sharpened to increase the sensitivity to the point where the current is almost too small to measure. The simple manufacturing method should allow commodity level production of highly sensitive electronic biosensors.     

In silico design of small RNA switches

The discovery of natural RNA sensors that respond to a change in the environment by a conformational switch can be utilized for various biotechnological and nanobiotechnological advances. One class of RNA sensors is the riboswitch: an RNA genetic control element that is capable of sensing small molecules, responding to a deviation in ligand concentration with a structural change. Riboswitches are modularly built from smaller components. Computational methods can potentially be utilized in assembling these building block components and offering improvements in the biochemical design process. We describe a computational procedure to design RNA switches from building blocks with favorable properties. To achieve maximal throughput for genetic control purposes, future designer RNA switches can be assembled based on a computerized preprocessing buildup of the constituent domains, namely the aptamer and the expression platform in the case of a synthetic riboswitch. Conformational switching is enabled by the RNA versatility to possess two highly stable states that are energetically close to each other but topologically distinct, separated by an energy barrier between them. Initially, computer simulations can produce a list of short sequences that switch between two conformers when trigerred by point mutations or temperature. The short sequences should possess an additional desirable property; when these selected small RNA switch segments are attached to various aptamers, the ligand binding mechanism should replace the aforementioned event triggers, which will no longer be effective for crossing the energy barrier. In the assembled RNA sequence, energy minimization folding predictions should then show no difference between the folded structure of the entire sequence relative to the folded structure of each of its constituents. Moreover, energy minimization methods applied on the entire sequence could aid at this preprocessing stage by exhibiting high mutational robustness to capture the stability of the formed hairpin in the expression platform. The above computer-assisted assembly procedure together with application specific considerations may further be tailored for therapeutic gene regulation. Index Terms-Design of RNA switches, energy minimization methods, RNA folding predictions.     

Fatigue assessment of offshore wind turbines on monopile foundations using multi‐band modal expansion

Offshore wind turbines (OWTs) are subjected to both quasi‐static loads originating from variations in the thrust force and dynamic loads linked to turbulence, waves and turbine dynamics. Both types of loads contribute to fatigue life progression and thus define the turbine's age. As a structural health monitoring solution, one could thus directly measure the stress history at fatigue critical locations. However, for OWTs on monopile foundations some fatigue critical locations are located below the seabed. Installing strain sensors at these hotspots is therefore impossible for existing wind turbines. This measurement restriction is overcome by reconstructing the full‐field response of the structure based on the limited number of accelerometers and strain sensors (installed at a few easily accessible locations) and a calibrated finite element model of the system. The system model uses a multi‐band modal expansion approach constituted of the quasi‐static and dynamic contributions. These contributions are superimposed to reconstruct the stress history at all degrees of freedom of the finite element model, and the subsequent assess fatigue life consumption at all fatigue hot spots of the OWT. In this paper, the proposed virtual sensing technique is validated by predicting the stresses in the transition piece with 12 days of consecutive measurements from an operational OWT. The data set contains both variations in environmental and operating conditions as well as extreme events. Finally, a full‐field strain assessment in the tower and foundation system of the OWT is demonstrated. Copyright © 2017 John Wiley & Sons, Ltd.     

A TRNSYS-based simulation framework for the analysis of solar thermal and heat pump systems

The modeling and simulation of combined solar and heat pump (SHP) systems is a challenging task as it requires expert knowledge in modeling as well as in the behavior of the real systems. As an example of a SHP system, this work considers parallel solar thermal and heat pump systems with ground or air source heat pumps for the efficient energy supply of buildings. An introduction to SHP systems and the considered system concepts is given and the challenges in designing new models within the simulation environment TRNSYS are described. Finally, a TRNSYS-based stand-alone tool (SHP-SimFrame) ispresented which enables users to analyze predefined SHP concepts with hardly any knowledge in modeling and simulation itself.