Proteinases in renal cell death

The role of proteinases in renal proximal tubule (RPT) cellular death was examined using specific inhibitors of proteinases. Rabbit RPT suspensions were incubated with antimycin A for 1 h or tetrafluoroethyl-L-cysteine (TFEC) for 4 h in the absence or presence of the specific cysteine proteinase inhibitor L-trans-epoxysuccinyl-leucylamido (4-guanidino)butane (E-64), the serine proteinase inhibitors N-p-tosyl-L-lysine chloromethyl ketone (TLCK) or 3,4-dichloroisocoumarin (DCS), the serine and cysteine proteinase inhibitors leupeptin or antipain, or the aspartic proteinase inhibitor pepstatin. E-64 and pepstatin decreased lactate dehydrogenase (LDH) release, a marker of cell death, from RPT exposed either to antimycin A or TFEC. TLCK, DCS, leupeptin, or antipain did not decrease antimycin A- or TFEC-induced cell death. Bromohydroquinone- or t-butylhydroperoxide-induced cell death was not decreased by any of the proteinase inhibitors. Loss of lysosomal membrane potential, indicated by neutral red release, occurred prior to the onset of antimycin A-induced cell death. Extensive inhibition of lysosomal cathepsins B and L by E-64 was correlated with cytoprotection. However, E-64 was only protective after some cell death had occurred. These results suggest that lysosomal cysteine and aspartic proteinases, but not serine proteinases, play a role in RPT cell death induced by antimycin A or TFEC. The observation that E-64 was only protective after some cell death had occurred suggests that lysosomal cathepsins are released from dying cells and subsequently attack the remaining viable cells.     

用复合烷基锂引发丁二烯聚合

对以环己烷为溶剂、四氢呋喃为微观结构调节剂、复合烷基锂为引发剂的丁二烯聚合反应过程进行了研究，考察了不同引发温度、不同配比的复合烷基锂对聚合反应的影响，并与以正丁基锂为引发剂的丁二烯聚合反应过程进行了比较。     

生物降解塑料开发现状与发展趋势

本文简要介绍国内外生物降解塑料的现状与发展趋势。     

自适应有源噪声控制的滤波-LMAD算法

本文研究了稳定非高斯环境下的自适应有源噪声控制问题，提出了滤波－ＬＭＡＤ（ｌｅａｓｔｍｅａｎａｂｓｏｌｕｔｅｄｅｖｉａｔｉｏｎ）算法及归一化滤波－ＬＭＡＤ算法，仿真结果表明，与常用的滤波－ＬＭＳ算法和归－化滤波－ＬＭＳ算法相比，本文提出了算法收敛速度更快。     

离散事件动态系统的代数模型及其控制器的分析计算

本文在离散事件动态系统的Ｐｅｔｒｉ网图形描述的基础上建立了相应的代数模型，该模型可以用来计算无竞争无回路Ｐｅｔｒｉ网的“最大状态”；该“最大状态”的计算在本文给出的动态反馈控制器中得到了应用。     

光盘用光学头物镜性能的综合评估

论述了采用波象差均方根、点扩展函数、光学传递函数对高次旋转对称非球面光学头物镜性能进行综合评估的方法。作者在正则坐标理论基础〔１〕上编制了光学头物镜性能综合评估软件。给出了评估实例。     

基于证据理论处理移动机器人视觉信息的一种方法

本文阐述了一种表示多源视觉传感信息的有效方法和一种基于Ｄｅｍｐｓｔｅｒ－Ｓｈａｆｅｒ证据理论的多传感器信息融合技术。着重分析了移动机器人视觉传感器信息中存在的相关性及其解决方法，提出了相关函数、相关证据等概念，并针对证据之间存在的相关性给出了一种改进的综合函数。     

ELNF演算的解释技术

ＥＬＮＦ演算是我们在ＬＮＦ演算的基础上扩充逻辑程序设计能力而得到的一种函数／逻辑演算系统，它构成了作者设计的函数／逻辑语言ＲＦＵＮＬＯＧ的基础．本文介绍ＥＬＮＦ演算的解释实现技术，包括数据结构、系统结构以及各个模块的设计思想．最后给出了在此解释系统下，几个典型程序的运行时间．     

自身相位噪声测量的分析

本文分析一种不需要参考（标准）信号源的相位噪声测量系统，该系统在被测通道里插入窄带晶体滤波器滤除被测信号的大部分噪声，作为参考信号再与被测信号进行比较，实现了信号源自身相位噪声的测量．本文还介绍一种自动相位噪声谱测量装置