基于“CDIO”的“1+2”新教学模式研究

“CDIO”教学是将工程思维训练融合在教学各个环节当中,本文采用“CDIO”与“案例教学、启发教学”进行融合,组合成为一个新的“1+2”教学模式,以医学信息工程学院课程作为应用载体,将传统教学升华到工程思维教学、团队教学上,转变教师授课形式,激励和引导学生自主学习,为学生提供思维的空间和意境,帮助学生在学习时发现问题,发现、解决值得解决的问题,掌握计算在问题解决过程中发挥的作用,获得构建自身学科知识体系,终身自我学习的能力,滋生创新能力,达到学科知识与工程思维能力互相促进,共同提高的目的。     

医学信息工程专业毕业设计过程质量控制的研究

毕业设计是实践教学的重要环节,由于其教学进度涉及的时间跨度较长,且教学过程多数贯穿在学生实习、考研及就业准备过程中,加之异地化的指导和管理较为困难,若缺乏行之有效的监督极易发生质量问题。本文以医学信息工程专业的毕业设计为例,从毕业设计各阶段的过程质量控制具体措施,配合线上教学管理平台的质量监督体系来研究切实提高毕业设计过程质量的有效办法。     

The rapid selecting of precursor ions and product ions of thirty-four kinds of pesticide for content determination by GC–EI/MS/MS

Pesticide residues caused great threat to human body health, and all countries protected the health of human body by specifying its upper limit. However, pesticide residues were generally very low, similar to trace analysis and as low as millionth or less, the content determination of pesticide residues was a troublesome problem. With the application and promotion of tandem mass spectrometry, it was able to do trace analysis. GC–EI/MS/MS was a common method for the determination of pesticide residues. The multiple reaction monitoring (MRM) mode was the most common quantitative method used in GC–EI/MS/MS. It has the characteristics of high sensitivity, good reproducibility, high accuracy, strong anti-interference and high ion flux. Multiple-twin precursor ions, product ions and collision energy of thirty-four pesticides were provided for MRM. It could play an important role in developing MRM method for the quantifying of 34 kinds of pesticide. Meanwhile, the development of MRM method for the quantifying of other compounds could also refer to this paper.     

A reliable and sensitive time-resolved fluorescent immunochromatographic assay (TRFICA) for ochratoxin A in agro-products

Immunochromatographic assays (ICAs) are considered as a suitable diagnostic tool for the detection of mycotoxins. Mycotoxins and especially, ochratoxin A are analytes with more demanding sensitivity requirements. To enhance the sensitivity of current immunochromatographic assays for ochratoxin A (OTA), a novel sensitive ICA was developed in this study. In the assay, microspheres enclosing fluorescent europium (III) [Eu(III)] nanoparticles (EuNPs) were used as a label for OTA monoclonal antibody (OTA-mAb) conjugation. Accordingly, assay was called time-resolved fluorescent immunochromatographic assay (TRFICA). The test strip was composed of three parts: a sample pad, nitrocellulose membrane and an absorbent pad. As for detection, a proper concentration of conjugated microspheres was pipetted into the microtube and sample extract was added to it. Then the strip was inserted into the tube and the fluid flow along the strip. The TRFICA results were obtained in 8 min and read by a portable TRFICA strip reader. The established method allows quantitative determination of OTA with limit of detection as low as 1.0 μg kg⁻¹ in the samples. For validation, spiked samples including wheat, maize, soybean and rice were respectively assayed by TRFICA and a standard high performance liquid chromatography equipped with a fluorescence detector (HPLC-FLD), and good agreement of results was obtained between two methods.     

Identification of Novel Pathways in Plant Lectin-Induced Cancer Cell Apoptosis

Plant lectins have been investigated to elucidate their complicated mechanisms due to their remarkable anticancer activities. Although plant lectins seems promising as a potential anticancer agent for further preclinical and clinical uses, further research is still urgently needed and should include more focus on molecular mechanisms. Herein, a Naïve Bayesian model was developed to predict the protein-protein interaction (PPI), and thus construct the global human PPI network. Moreover, multiple sources of biological data, such as smallest shared biological process (SSBP), domain-domain interaction (DDI), gene co-expression profiles and cross-species interolog mapping were integrated to build the core apoptotic PPI network. In addition, we further modified it into a plant lectin-induced apoptotic cell death context. Then, we identified 22 apoptotic hub proteins in mesothelioma cells according to their different microarray expressions. Subsequently, we used combinational methods to predict microRNAs (miRNAs) which could negatively regulate the abovementioned hub proteins. Together, we demonstrated the ability of our Naïve Bayesian model-based network for identifying novel plant lectin-treated cancer cell apoptotic pathways. These findings may provide new clues concerning plant lectins as potential apoptotic inducers for cancer drug discovery.     

微波预处理提取白芷中总香豆素的研究

目的研究提取白芷总香豆素的方法。方法采用微波预处理技术,考察药材粉碎粒径的大小、汽化剂浓度及用量、微波辐射时间、后续提取时间和后续提取溶剂浓度对提取率的影响,运用正交试验优化预处理工艺参数。结果确定提取工艺参数为:白芷药材过24目筛,汽化剂为60%乙醇,用量为2.5倍物料量(mL/g),辐射时间150 s,后续提取溶剂75%乙醇,常规回流提取2次,每次40 min,提取率可达到0.845%。结论此工艺稳定、合理、可行。     

大学生性心理教育培养模式的探索与构建

近年来,大学生因性心理困扰造成的突发事件逐渐增多,对校园稳定、教学秩序、大学生心理健康等造成不良影响。随着我国社会深刻转型和改革开放的快速发展,建立一套适合大学生自身特点的健康性心理教育培养模式非常必要。从医类大学生特点的角度,对大学生健康性心理教育培养模式进行了理论探索,提出了推进健康性心理教育的紧迫性和必要性,并论证了对大学生发展自我、完善自我、提高心理自我保护意识具有重要的指导意义。     

Heat Transfer and Deformation Behavior of Shell Solidification in Wide and Thick Slab Continuous Casting Mold

With the considerations of the behaviors of shell deformation, mold flux film and air gap dynamic distribution in shell/mold gap, a two dimensional slice-travel transient thermo-mechanical coupled model of simulation shell solidification in wide and thick slab continuous casting mold was developed by using the commercial program ANSYS. The evolutions of strand-mold system thermal behaviors, including the air gap formation and the mold flux film dynamical distribution in shell/mold gap and shell temperature field, and the evolutions of shell deformation and stress distribution of peritectic steel solidified in a 2120 mm wide and 266 mm thick slab continuous casting mold were analyzed. The results show that the air gap formation and the thick mold flux film distribution mainly concentrate in the regions 0–21 mm and 0–7 mm, 0–120 mm and 0–100 mm off the shell wide and narrow faces corners, and thus the hot spots are given rise to form in the regions 15–55 mm and 15–50 mm off the shell wide and narrow face corners. The shell server deformation occurs in the off-corners in the middle and lower parts of the mold. The stress evolution in shell surface is tensile stress, while that in shell solidification front is compressive stress.