小径落叶松材制作空间网架杆件的选择与设计

通过对小径落叶松材的力学性能、与金属套筒连接性能及胶粘剂的选择试验,设计用小径落叶松材制作空间网架,扩大了小径材在建筑领域的使用范围。     

氧糖剥夺条件下PC12细胞RAGE的表达及对其损伤的影响

目的探讨大鼠肾上腺嗜铬细胞瘤(PC12)细胞在氧糖剥夺(OGD)的条件下,晚期糖基化终产物受体(RAGE)的表达及其对PC12细胞损伤的影响。方法PC12细胞随机分为3组:OGD培养组(PC12细胞在无血清无糖的DMEM培养液中厌氧培养);封闭RAGE的OGD培养组(PC12细胞在加5μg/mlRAGE抗体的无血清无糖DMEM培养液中厌氧培养);对照组(PC12细胞在无血清无糖DMEM培养液中培养)。免疫组化法检测RAGE表达。收集细胞上清液,检测乳酸脱氢酶(LDH)活性和一氧化氮(NO)含量,并对细胞死亡率进行检测。结果在OGD条件下PC12细胞培养8、11、20h均有RAGE表达,与对照组相比表达均明显增加,OGD培养组与封闭RAGEOGD培养组相比,LDH活性差异显著。随着厌氧时间的延长,PC12细胞死亡率明显增高,封闭RAGEOGD培养组与OGD培养组相比,PC12细胞死亡率明显降低。NO含量差异无显著意义,但与对照组相比差异显著。结论在OGD条件下,PC12细胞RAGE表达增高。RAGE的表达对细胞的损伤起促进作用。     

职业能力导向下的高职公共英语师资队伍建设探究

在分析研究高职院校公共英语教师队伍现状的基础上,就公共英语师资的职业化建设方面提出如何加强职业能力建设,掌握扎实的理论知识和良好的教育教学技能及丰富的专业实践能力等建议,以期促进复合型高职教师的培养。     

Nontoxic engineered virus nanofibers as an efficient agent for the prevention and detection of fungal infection

Nano Research - Candida albicans (C. albicans) infection has a high mortality rate in immunocompromised patients. Owing to the inefficiency of the current diagnostic system and the absence of...     

球形孔洞膨胀动态问题的弹性-损伤力学分析

研究了材料还没有出现塑性变形、仅含弹性区和损伤区的球形孔洞动态扩展问题．首先通过对弹性区的研究以及初始损伤分析获得弹性区的场量分布,并给出弹性／损伤区交界处的边界连续条件;然后在自相似假设条件下,推导出动态扩展时损伤区需满足的控制方程;最后通过打靶法进行数值求解．数值分析表明,许多材料参数如ν、n、m都对弹性区和损伤区的场量分布有影响．     

Spatial interpolation methods applied in the environmental sciences: A review

Spatially continuous data of environmental variables are often required for environmental sciences and management. However, information for environmental variables is usually collected by point sampling, particularly for the mountainous region and deep ocean area. Thus, methods generating such spatially continuous data by using point samples become essential tools. Spatial interpolation methods (SIMs) are, however, often data-specific or even variable-specific. Many factors affect the predictive performance of the methods and previous studies have shown that their effects are not consistent. Hence it is difficult to select an appropriate method for a given dataset. This review aims to provide guidelines and suggestions regarding application of SIMs to environmental data by comparing the features of the commonly applied methods which fall into three categories, namely: non-geostatistical interpolation methods, geostatistical interpolation methods and combined methods. Factors affecting the performance, including sampling design, sample spatial distribution, data quality, correlation between primary and secondary variables, and interaction among factors, are discussed. A total of 25 commonly applied methods are then classified based on their features to provide an overview of the relationships among them. These features are quantified and then clustered to show similarities among these 25 methods. An easy to use decision tree for selecting an appropriate method from these 25 methods is developed based on data availability, data nature, expected estimation, and features of the method. Finally, a list of software packages for spatial interpolation is provided.     

An investigation on the dosimetric impact of hip prosthesis in radiotherapy

The objectives of this study are to evaluate the dosimetric impact of hip prosthesis using ELEKTA linac X-rays and compare the dose perturbations of metallic and nonmetallic hip prostheses. The local dose distributions of typical hip prostheses were calculated for 4, 8, and 15 MV beams by Geant4. Three prosthesis materials were selected in calculation to reveal the relation between material type and local dose perturbations of prostheses. Furthermore,the effect of nominal energy on prosthesis perturbation was also discussed and analyzed. Taking the calculated dose to the hip joint as reference, considerable differences were observed between prostheses and hip joints. In the prosthesis shadow region, the relative dose decreasing was up to 36, 21, and 16 % for the Co–Cr–Mo alloy, titanium alloy, and ceramic prostheses, respectively. In backscattering region, the relative dose increasing was about1–7 %. Overall, the results show that the dose perturbation effect of prostheses was mainly determined by material type, nominal energy, and density. Among these typical hip prostheses, ceramic prosthesis introduces the lowest dose perturbations.     

GPC1 Regulated by miR-96-5p,Rather than miR-182-5p,in Inhibition of Pancreatic Carcinoma Cell Proliferation

To determine the relationships between miR-96-5p/-182-5p and GPC1 in pancreatic cancer (PC), we conducted the population and in vitro studies. We followed 38 pancreatic cancer patients, measured and compared the expression of miR-96-5p/-182-5p, GPC1, characteristics and patients’ survival time of different miR-96-5p/-182-5p expression levels in PC tissues. In an in vitro study, we investigated the proliferation, cycle and apotosis in cells transfected with mimics/inhibitors of the two miRNAs, and determine their effects on GPC1 by dual-luciferase assay. In the follow-up study, we found that the expressions of miR-96-5p/-182-5p were lower/higher in PC tissues; patients with lower/higher levels of miR-96-5p/-182-5p suffered poorer characteristics and decreased survival time. In the in vitro study, the expressions of miR-96-5p/-182-5p were different in cells. Proliferation of cells transfected with miR-96-5p mimics/inhibitors was lower/higher in Panc-1/BxPC-3; when transfected with miR-182-5p mimics/inhibitors, proliferation of cells were higher/lower in AsPC-1/Panc-1. In a cell cycle study, panc-1 cells transfected with miR-96-5p mimics was arrested at G0/G1; BxPC-3 cells transfected with miR-96-5p inhibitors showed a significantly decrease at G0/G1; AsPC-1 cells transfected with miR-182-5p mimics was arrested at S; Panc-1 cells transfected with miR-182-5p inhibitors showed a decrease at S. MiR-96-5p mimics increased the apoptosis rate in Panc-1 cells, and its inhibitors decreased the apoptosis rate in BxPC-3. Dual luciferase assay revealed that GPC1 was regulated by miR-96-5p, not -182-5p. We found that miR-96-5p/-182-5p as good markers for PC; miR-96-5p, rather than -182-5p, inhibits GPC1 to suppress proliferation of PC cells.     

转化生长因子-β在骨髓间充质干细胞治疗实验性自身免疫性重症肌无力机制中的作用

目的探讨转化生长因子-β(TGF-β)在骨髓间充质干细胞(BMSCs)治疗实验性自身免疫性重症肌无力(EAMG)机制中的作用。方法分离培养健康Lewis大鼠BMSCs,并进行大量体外扩增;以大鼠来源的乙酰胆碱受体(R-AChR)2次免疫Lewis大鼠,建立EAMG模型;第2次免疫的同时,经尾静脉移植BMSCs,1×107个/只,依据Lennon评分标准,进行体重测量和临床体征评定。并通过体外实验进一步探讨TGF-β在治疗EAMG过程中的具体机制。结果BMSCs移植明显缓解了EAMG的临床症状,临床评分及体重变化差异均有统计学意义,表明治疗有效;体外实验结果显示,BMSCs能通过TGF-β的分泌影响AChR特异性Th17/Treg细胞亚群的分布及其相关因子的分泌,以anti-TGF-β抗体封闭后,这种调节作用在一定程度上被抑制。结论BMSCs通过细胞因子TGF-β,能在一定程度上调节AChR特异性Th17/Treg细胞亚群的平衡,从而起到治疗EAMG的作用。     

建筑工程技术专业人才培养方案的开发与实践

结合我校建筑工程技术专业人才培养方案的开发与实践经验,对建筑工程技术专业人才培养方案的开发理念、原则、流程及内容进行了重点探讨,取得了预期的效果。