Strain variability of the behavior of foodborne bacterial pathogens: A review

Differences in phenotypic responses among strains of the same microbial species constitute an important source of variability in microbiological studies, and as such they need to be assessed, characterized and taken into account. This review provides a compilation of available research data on the strain variability of four basic behavioral aspects of foodborne bacterial pathogens including: (i) virulence; (ii) growth; (iii) inactivation; and (iv) biofilm formation. A particular emphasis is placed on the foodborne pathogens Listeria monocytogenes and Salmonella enterica. The implications of strain variability for food safety challenge studies and microbial risk assessment are discussed also. The information provided indicates that the variability among strains of foodborne bacterial pathogens with respect to their behavior can be significant and should not be overlooked. However, in order for the mechanisms underlying the observed strain variability to be elucidated and understood, phenotypic variability data, such as those reviewed here, should be evaluated in conjunction with corresponding findings of studies assessing the molecular/physiological basis of this variability.     

Simulations of THM processes in buffer-rock barriers of high-level waste disposal in an argillaceous formation

The main objective of this paper is to investigate and analyse the thermo-hydro-mechanical (THM) coupling phenomena and their influences on the repository safety. In this paper, the high-level waste (HLW) disposal concept in drifts in clay formation with backfilled bentonite buffer is represented numerically using the CODE_BRIGHT developed by the Technical University of Catalonia in Barcelona. The parameters of clay and bentonite used in the simulation are determined by laboratory and in situ experiments. The calculation results are presented to show the hydro-mechanical (HM) processes during the operation phase and the THM processes in the after-closure phase. According to the simulation results, the most probable critical processes for the disposal project have been represented and analyzed. The work also provides an input for additional development regarding the design, assessment and validation of the HLW disposal concept.     

Robust nonlinear control of spacecraft formation flying using constraint forces

A robust nonlinear control method is presented for spacecraft precise formation flying.With the constraint forces and consid-ering nonlinearity and perturbations,the problem of the formation keeping is changed to the Lagrange systems with the holonomic constraints and the differential algebraic equations (DAE).The nonlinear control laws are developed by solving the DAE.Because the traditional numerical solving methods of DAE are very sensitive to the various errors and the resulting con-trol laws are not ro...     

FL凹陷复杂水系下高电阻率储层油气评价技术

介绍利用自然电位幅度差值△SP与视地层水电阻率Rwa交会图确定复杂地层水系下高电阻率地层的地层水电阻率的方法，并计算储层含油饱和度，判断油水地层。以实例证明该方法在FL凹陷的应用。     

载运类工程机械性格形成和发展研究

为了寻求载运类工程机械的共性特点，有效地指导产品外观形象设计，首次提出了工程机械性格的概念，重点研究了载运类工程机械的性格形成过程和发展趋势。通过对典型产品性格变化与社会需求、技术水平之间关系的梳理和相关文献的整理，认为现代载运类工程机械的性格特点是刚强、沉稳、机敏，其性格形成经历了性格孕育期、萌芽期、塑造期和成熟期4个阶段。     

One step gold (bio)functionalisation based on CS2-amine reaction

Dithiocarbamates have been regarded as alternative anchor groups to thiols on gold surfaces, and claimed to be formed in situ through the reaction between secondary amines and carbon disulphide. In this paper, we further exploit this methodology for a convenient one step biomolecule immobilisation onto gold surfaces. First, the reactivity between CS₂ and electroactive compounds containing amines, primary (dopamine), secondary (epinephrine), and an amino acid (tryptophan) has been investigated by electrochemical methods. Cyclic voltammetric characterisation of the modified electrodes confirmed the immobilisation of all the target compounds, allowing the estimation of their surface concentration. The best result was obtained with epinephrine, a secondary amine, for which a typical quasi-reversible behaviour of surface confined electroactive species could be clearly depicted. Electrochemical reductive desorption studies enabled to infer on the extent of the reaction and on the relative stability of the generated monolayers. Bio-functionalisation studies have been accomplished through the reaction of CS₂ with glucose oxidase in aqueous medium, and the catalytic activity of the immobilised enzyme was evaluated towards glucose, by electrochemical methods in the presence of a redox mediator. Scanning tunnelling microscopy (STM) and Atomic force microscopy (AFM) were used respectively, to characterize the gold electrodes and Glucose Oxidase coverage and distribution on the modified surfaces.     

Hydrogen solid storage: First-principles study of ZrNiH3

The ZrNiH₃ compound is a good candidate for hydrogen storage. In this work we used the first-principles calculation to study this compound. The crystal structures, the electronic properties and the optimization of the internal parameters are treated by the FP-LAPW method implanted in the WIEN2K code. The enthalpies of the dehydrogenation of the ZrNiH₃ compound are calculated. We found that the enthalpy is about −42.89 kJ/mol H, greater but similar to the experimental value of −34.3 kJ/mol H. Potential reasons for this discrepancy are discussed.     

Manufacturing cell formation with production data using neural networks

Batch type production strategies need adoption of cellular manufacturing (CM) in order to improve operational effectiveness by reducing manufacturing lead time and costs related to inventory and material handling. CM necessitates that parts are to be grouped into part families based on their similarities in manufacturing and design attributes. Then, machines are allocated into machine cells to produce the identified part families so that productivity and flexibility of the system can be improved. Zero-one part-machine incidence matrix (PMIM) generated from route sheet information is commonly presented as input for clustering of parts and machines. An entry of ‘1’ in PMIM indicates that the part is visiting the machine and zero otherwise. The output is generated in the form of block diagonal structure where each block represents a machine cell having more than one machines and a part family. The major limitations of this approach lies in the fact that important production factors like operation time, sequence of operations, and lot size of the parts are not accounted for. In this paper, an attempt has been made to propose a clustering methodology based on adaptive resonance theory (ART) for addressing these issues. Initially, a methodology considering only the operation sequence of the parts has been proposed. Then, the methodology is suitably modified to deal with combination of operation sequence and operation time of the parts to address generalized cell formation (CF) problem. A new performance measure is proposed to quantify the performance of the proposed methodology. The performance of the proposed algorithm is tested with benchmark problems from open literature and the results are compared with the existing methods. The results clearly indicate that the proposed methodology outperforms the existing methods in most cases.     

煤炭销售计量用机械轨道衡的电子化改造

本文介绍了静态机械轨道的电子化改造技术，给出了改造原理、方法和称重传感器、称重仪表的选择依据，计算方法以及仪表系统设计原理。     

关于金刚石合成棒中白色物质形成机理的研究

对金刚石合成棒中白色物质的研究表明，它是在高温高压条件下搞入合成棒的叶腊石与触煤合金发生一系列复杂反应而使触媒渣化的产物。文中对触媒渣化的热力学、动力学亦作了分析。