抗rHu-IFN-αMcAb亲和常数的测定

采用ELISA间接法检测抗原－抗体反应系统中游离抗体量的方法，对本实验室制备的5系抗rHu－IFN－αMcAb进行了亲和力测定，获得比较准确的McAb亲和常数。     

α-蒎烯阳离子聚合工艺的改进

以A lC l3/SbC l3为催化剂,用SbC l3逐步滴加方式,通过两步聚合工艺制备了α-蒎烯树脂。通过正交实验,对α-蒎烯调控聚合的各影响因素进行了研究。结果表明,与传统的一步聚合工艺相比,该工艺在保持单体高转化率的同时,使聚合反应变缓,避免了由于反应过快而引起的聚合产物中低聚体含量高,Mn下降的现象;有效提高了聚合物的软化点;降低了聚合物的色值。在优化工艺条件下,催化剂用量比传统工艺减少30%以上,制得的α-蒎烯树脂软化点为138℃(环球法),色泽3(铁钴色),得率达81.0%,软化点提高8℃以上,色泽降低2级以上。产品各项指标均超过了我国行业标准LY/T1453—1999特级水平。     

Integrative Extraction of Ergosterol, （1→3）-α-D-Glucan and Chitosan from Penicillium chrysogenum Mycelia

Abstract Ergosterol,（1→3）-α-D-glucan and chitosan are important biomaterials. In this research, a process has been developed to integratively extract ergosterol, （1→3）-α-D-glucan, and chitosan from Penicillium chrysongenum mycelium. First the mycelia are pretreated with 0.1mol·L^-1 of NaOH. After recovery by centrifugation the solid portion is made to undergo saponification and deacetylation reactaons by addition of 2mol·L^-1 NaOH and et anol.After reaction, extraction is carried out by addition of petroleum ether, which separates the reaction mixture into two phases. The upper layer of petroleum ether contains extracted ergosterol, and the .bottom layer of NaOH solution contains （1→3）-α-DEglucan; the chitosan is on the mycelia residuum. After isolation, the recovery yield of ergosterol is 0.52% of dry mycelium. That of （1→3）-α-D-glucan is about 8.2%; and chitosan is 5.7% with 86% deacetylation. The compositions have been characterized by 1R, HPLC analyses.     

A new generalised α scale spaces quadrature filters

The α   scale spaces is a recent theory that open new possibilities of phase-based image processing. It is a parameterised class (α∈]0,1])$(\mathrm{<font\; color="red">\alpha </font>}\in ]0,1\left]\right)$ of linear scale space representations, which allows a continuous connection beyond the well-known Gaussian scale space (α=1$\mathrm{<font\; color="red">\alpha </font>}=1$). In this paper, we make use of this unified representation to derive new families of band pass quadrature filters, built from derivatives and difference of the α scale space generating kernels. This construction leads to generalised α kernel filters including the commonly known families derived from the Gaussian and the Poisson kernels. The properties of each family are first studied and then experiments on one- and two-dimensional signals are shown to exemplify how the suggested filters can be used for edge detection. This work is complemented by an experimental evaluation, which demonstrates that the new proposed filters are a good alternative to the commonly used Log-Gabor filter.     

2-苯基-4-对氯苯基-1,5-苯并硫氮杂-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法分析

采用Hypersil-ODS2(4.6mm×250mm,5μm)色谱柱,乙腈-水(70∶30)流动相,242nm检测波长,建立了测定2-苯基-4-对氯苯基-1,5-苯并硫氮杂-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法。该法线性范为0～0.1mg/mL,回归方程为A=1.63×108c-2.86×105,相关系数r=0.9994,相对标准偏差为0.33%(c=0.06mg/mL,n=5),检出限为0.08μg/mL。平均回收率在97.2%～102.4%之间。该法重现性好,灵敏度高,简单,快速,准确。     

电子封装用环氧树脂的增韧和提高耐热性研究

用α,ω 二氯聚二甲基硅氧烷(DPS)或α 氯聚二甲基硅氧烷(CPS)来改性普通双酚A环氧树脂(BPAER)和四溴双酚A环氧树脂(TBBPAER),目标是制备出一系列可用于电子封装的高韧性高耐热性的环氧基料。通过对固化物的冲击强度、拉伸强度、断裂伸长率和玻璃化转变温度(Tg)以及断裂面形态的测定,探讨了改性方法、有机硅组成与含量等对材料性能的影响。结果表明,当m(BPAER)∶m(DPS)=100∶10时,树脂固化物的冲击强度达到30 5kJ/m2,拉伸强度达46 95MPa,断裂伸长率达到60 23%,Tg达到141 3℃;分别比未改性BPAER提高了19 7kJ/m2,1 69MPa,54 29%以及5 9℃。而当m(TBBPAER)∶m(CPS)=100∶10时,固化物的冲击强度达到17 2kJ/m2,拉伸强度达39 89MPa,断裂伸长率达到5 60%,Tg达到147 0℃;分别比未改性TBBPAER提高了12 8kJ/m2,28 26MPa,4 29%以及7 9℃。     

基于LS-SVM逆系统的无轴承异步电机解耦控制

无轴承异步电机具有非线性、多变量和强耦合的特点,要实现电机稳定悬浮和旋转运行,必须对其进行非线性动态解耦控制。为了克服逆系统方法精确建模难的局限性,采用基于最小二乘支持向量机(LS-SVM)α阶逆系统方法对无轴承异步电机进行动态解耦控制的研究。首先利用最小二乘支持向量机辨识出无轴承异步电机的逆模型,然后将它串联在原系统前,将无轴承异步电机解耦成四个独立的伪线性子系统-2个径向位移子系统、一个速度子系统和一个磁链子系统。为保证鲁棒性能,最后对解耦后的系统采用非线性内模控制策略。研究表明,LS-SVMα阶逆系统方法能够实现无轴承异步电机径向悬浮力和旋转力之间的动态解耦控制,控制系统具有良好的静态和动态性能。     

α-取代十四酸衍生物的合成

报道了三种α－取代十四酸衍生物的合成方法，通过元素分析及波谱分析确证了新化合物α－（对硝基苯氧基）十四酸的结构。     

Influence of free volume fraction,inter/intramolecular energy ratio,and chain segment density on the Gibbs-DiMarzio theory of the glass transition

Based upon computer analysis, the Gibbs-DiMarzio (G-DM) theory is evaluated for the case of (a) constant V₀ and (b) constant r where V₀ is the fractional free volume at T_g and r is the ratio of the hole (E₀) to the flex (ε) energy. For each respective case, results are presented in a reduced variables format that indicate the extent to which r and V₀ vary at the transition temperature as a function of the reciprocal degree of polymerization (P^?1). To further account for chemical differences that exist among polymers, an index (n) is introduced that ultimately incorporates the effective number average of chain atom segments (x?) per P?. Using these reduced variables plots, the effects of V₀, r, and n are compared with the T_g data for four well-documented polymers. Although the theory adequately describes the PMMA, PS, and PVC data, for PαMS the fit is doubtful. The analysis demonstrates that, in order to maintain V₀ within the range of 0.015–0.045, r must remain within approximately 1.0–1.1. Moreover, under conditions of either constant V₀ or r, other more flexible polymers require that $\text{n?10}$.     

无标样RP-HPLC测定α-吡咯烷酮乙酸甲酯

α-吡咯烷酮乙酸甲酯是一种医药中间体,建立了一种RP-HPLC方法,可以在没有α-吡咯烷酮乙酸甲酯标准对照品的情况下,测定合成样品中α-吡咯烷酮乙酸甲酯的含量,当α-吡咯烷酮乙酸甲酯含量为91%~96%时,SD为0.63%~0.82%,RSD为0.66%~0.89%,与化学分析法对比,结果令人满意。